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Phosphohistidine Phosphatase 1 (PHPT1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2170657

MBS2170657 is an antibody targeting PHPT1. Also known as Anti-Phosphohistidine Phosphatase 1 (PHPT1) Polyclonal Antibody. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phosphohistidine Phosphatase 1 (PHPT1), Polyclonal Antibody
Full Product Name
Anti-Phosphohistidine Phosphatase 1 (PHPT1) Polyclonal Antibody
Product Gene Name
Product Synonym Names
Anti-Phosphohistidine Phosphatase 1; Anti-Phosphohistidine Phosphatase 1 (PHPT1) Polyclonal Antibody; PHP14; Phosphohistidine Phosphatase 14kDa; Sex-Regulated Protein Janus-a; Protein janus-A homolog
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.5mg/mL (lot specific)
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Molecular Weight
125
OMIM
610167
3D Structure
ModBase 3D Structure for Q9NRX4
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Research Area
Enzyme & Kinase

Product Categories/Family for anti-PHPT1 antibody

ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PHPT1 antibody
Flow cytometry
Application Notes for anti-PHPT1 antibody
FC/FACS/FCM: 20ug/ml

Flow Cytometry (FC/FACS) of anti-PHPT1 antibody

Human THP1 cell line was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 20ug/ml rabbit Anti-human PHPT1 Polyclonal Antibody ( #MBS2170657, red histogram) or Isotype control antibody (#MBS2140235, green histogram), followed by 1ug/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (#MBS2086046).
Rabbit Anti-PHPT1 Polyclonal Antibody – Cat# MBS2170657

Other Notes

Small volumes of anti-PHPT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PHPT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
14 kDa phosphohistidine phosphatase
NCBI Official Synonym Full Names
phosphohistidine phosphatase 1
NCBI Official Symbol
PHPT1
NCBI Official Synonym Symbols
PHP14; CGI-202; HSPC141; HEL-S-132P
UniProt Protein Name
14 kDa phosphohistidine phosphatase
UniProt Synonym Protein Names
Phosphohistidine phosphatase 1; Protein janus-A homolog
UniProt Gene Name
PHPT1
UniProt Synonym Gene Names
PHP14
UniProt Entry Name
PHP14_HUMAN
NCBI Protein Information
14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P

NCBI Summary for PHPT1

This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
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UniProt Comments for PHPT1

PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.

Protein type: Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function: phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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