Mouse Anti-CD14 Monoclonal Antibody – Cat# MBS217377

Staining of mouse peripheral blood monocytes with MOUSE ANTI MOUSE CD14:RPE

CD14 Antibody – Mouse Monoclonal

Name: CD14 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS217377
Price: 560 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS217377

Mouse anti Mouse CD14 antibody, clone biG57 recognizes mouse CD14, a 55kD a glycophosphoinositol-linked cell surface protein that also exists in a soluble form. Expression of CD14 is restricted to the myeloid lineage and is predominantly expressed on… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CD14, Monoclonal Antibody

Full Product Name

MOUSE ANTI MOUSE CD14:RPE

Product Gene Name

anti-CD14 antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

biG57

Host

Mouse

Form/Format

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Preparation and Storage

Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

Molecular Weight

39,204 Da

3D Structure

ModBase 3D Structure for P10810

Reconstitution

Reconstitute with 1.0ml distilled water
Perservative Stabilisers||0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein G

Immunogen

Recombinant mouse CD14
Buffer Solution||Phosphate buffered saline

Target Species

Mouse

Applications Tested/Suitable for anti-CD14 antibody

Flow cytometry (FC/FACS)

Application Notes for anti-CD14 antibody

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Maximum Dilution: neat

Other Notes

Small volumes of anti-CD14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD14 purchase

MBS217377 is an antibody targeting CD14. Also known as MOUSE ANTI MOUSE CD14:RPE. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.

NCBI GI #

6753332

NCBI GeneID

12475

NCBI Accession #

NP_033971.1

UniProt Primary Accession #

P10810

UniProt Related Accession #

P10810

GenBank Nucleotide #

NM_009841.4

NCBI Official Full Name

monocyte differentiation antigen CD14

NCBI Official Synonym Full Names

CD14 antigen

NCBI Official Symbol

Cd14

UniProt Protein Name

Monocyte differentiation antigen CD14

UniProt Synonym Protein Names

Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14

Protein Family

Monocyte differentiation antigen

UniProt Gene Name

Cd14

UniProt Entry Name

CD14_MOUSE

NCBI Protein Information

monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein

NCBI Summary for CD14

This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]

UniProt Comments for CD14

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Cellular Component: extracellular space; cell surface; membrane; plasma membrane; lipid raft

Molecular Function: lipopolysaccharide binding

Biological Process: immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; inflammatory response; positive regulation of tumor necrosis factor production; positive regulation of cytokine secretion

Research Articles on CD14

1. Data show that ingestion of sCD14 resulted in blood sCD14 levels up.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD14 antibody

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies	Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
IKK Complex Recruitment Mediated By RIP1 Pathway antibodies	IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Legionellosis Pathway antibodies	Legionellosis Pathway Diagram

Diseases associated with anti-CD14 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>2319 publications with CD14 and Inflammation
Necrosis Antibodies	>2310 publications with CD14 and Necrosis
Cardiovascular Diseases Antibodies	>647 publications with CD14 and Cardiovascular Diseases
Lung Diseases Antibodies	>531 publications with CD14 and Lung Diseases
Liver Diseases Antibodies	>360 publications with CD14 and Liver Diseases
Nervous System Diseases Antibodies	>359 publications with CD14 and Nervous System Diseases
Disease Models, Animal Antibodies	>330 publications with CD14 and Disease Models, Animal
Atherosclerosis Antibodies	>311 publications with CD14 and Atherosclerosis
Heart Diseases Antibodies	>280 publications with CD14 and Heart Diseases
Asthma Antibodies	>259 publications with CD14 and Asthma

Organs/Tissues associated with anti-CD14 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7524 publications with CD14 and Blood
Bone Antibodies	>1329 publications with CD14 and Bone
Bone Marrow Antibodies	>1069 publications with CD14 and Bone Marrow
Liver Antibodies	>622 publications with CD14 and Liver
Lung Antibodies	>597 publications with CD14 and Lung
Skin Antibodies	>407 publications with CD14 and Skin
Kidney Antibodies	>334 publications with CD14 and Kidney
Brain Antibodies	>307 publications with CD14 and Brain
Muscle Antibodies	>281 publications with CD14 and Muscle
Joint Antibodies	>278 publications with CD14 and Joint