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CD257 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439850

MBS439850 is an antibody targeting CD257. Also known as CD257/BAFF/TNFSF13B. Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD257/BAFF/TNFSF13B, Monoclonal Antibody
Full Product Name
CD257/BAFF/TNFSF13B
Product Gene Name
Product Synonym Gene Name
BAFF; TNFSF13B
Product Synonym Names
ApoL related ligand TALL 1; B lymphocyte stimulator; B-cell-activating factor; BAFF; BLyS; CD257; Dendritic cell-derived TNF-like molecule; DTL; PRO738; TALL-1; THANK; TNFSF13B; TNFSF20; Tumor necrosis factor ligand superfamily member 13b; Tumor necrosis factor ligand superfamily member 20; ZTNF4
Clonality
Monoclonal
Isotype
IgG1
Clone Number
C257/1638
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Specificity
BAFF/CD257 is a type II transmembrane protein, and a member of the tumor necrosis factor ligand superfamily. It is proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homo-trimers. The predicted molecular weight of a BAFF monomer is approximately 31kDa. It is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells and is upregulated by IFN-gamma and down-regulated by PMA/ionomycin treatment. BAFF stimulates B and T cell immunity and regulates humoral immunity by binding TACI and BCMA receptors; BAFF prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR/BR3).
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
31kDa (Monomer)
3D Structure
ModBase 3D Structure for Q9Y275
Immunogen
Purified recombinant human full-length CD257 protein
Cellular Localization
Cell Surface and Cytoplasmic
Hu-chromosome Location
13q32-q34
Positive Control
Jurkat cells. Tonsil and lymph node
Applications Tested/Suitable for anti-CD257 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-CD257 antibody
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml

SDS-Page of anti-CD257 antibody

SDS-PAGE Analysis Purified BCL-6 Mouse Monoclonal Antibody (BCL6/1718). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-CD257 Monoclonal Antibody – Cat# MBS439850

Product References and Citations for anti-CD257 antibody

Schneider P, et al. 1999. J. Exp. Med. 189:1747. Moore PA, et al. 1999. Science 285:260.

Other Notes

Small volumes of anti-CD257 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD257. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 13B isoform 2
NCBI Official Synonym Full Names
TNF superfamily member 13b
NCBI Official Symbol
TNFSF13B
NCBI Official Synonym Symbols
DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNLG7A; TNFSF20
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13B
UniProt Synonym Protein Names
B lymphocyte stimulator; BLyS
UniProt Gene Name
TNFSF13B
UniProt Synonym Gene Names
BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13B

NCBI Summary for CD257

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
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UniProt Comments for CD257

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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