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ESTROGEN RECEPTOR BETA 5 (ESR2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS219170

Mouse anti human estrogen receptor beta 5, clone 5/25 specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5)(Moore et al. 1998).… More details

$1095 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
ESTROGEN RECEPTOR BETA 5 (ESR2), Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5
Product Gene Name
anti-ESR2 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5/25
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
53,209 Da
OMIM
601663
3D Structure
ModBase 3D Structure for Q92731
Perservative Stabilisers
0.1% Sodium Azide (NaN3)
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Histology Positive Control Tissue
Normal colon or colon carcinoma
Buffer Solution||Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-ESR2 antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-ESR2 antibody
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Testing Data of anti-ESR2 antibody

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta5 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power)
Mouse Anti-ESR2 Monoclonal Antibody – Cat# MBS219170

Testing Data of anti-ESR2 antibody

Published customer image: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERa was low/absent in poor grade cancers (J) but immunoexpression of ERbeta1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERbeta1, ERbeta2 and ERbeta5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers.From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330.
Mouse Anti-ESR2 Monoclonal Antibody, Testing Data – Cat# MBS219170

Testing Data of anti-ESR2 antibody

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta5 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power)
Mouse Anti-ESR2 Monoclonal Antibody, Testing Data – Cat# MBS219170

Other Notes

Small volumes of anti-ESR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ESR2 purchase

MBS219170 is an antibody targeting ESR2. Also known as MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ESR2. It may not necessarily be applicable to this product.

NCBI GI #
94538325
NCBI GeneID
2100
NCBI Accession #
NP_001035365.1
UniProt Primary Accession #
Q92731
UniProt Secondary Accession #
O60608; O60685; O60702; O60703; O75583; O75584; Q0MWT5; Q0MWT6; Q86Z31; A8K8K5; G3V5M5
UniProt Related Accession #
Q92731
GenBank Nucleotide #
NM_001040275.1
NCBI Official Full Name
estrogen receptor beta isoform 2
NCBI Official Synonym Full Names
estrogen receptor 2 (ER beta)
NCBI Official Symbol
ESR2
NCBI Official Synonym Symbols
Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
UniProt Protein Name
Estrogen receptor beta
UniProt Synonym Protein Names
Nuclear receptor subfamily 3 group A member 2
Protein Family
Estrogen receptor
UniProt Gene Name
ESR2
UniProt Synonym Gene Names
ESTRB; NR3A2; ER-beta
UniProt Entry Name
ESR2_HUMAN
NCBI Protein Information
estrogen receptor beta; estrogen receptor beta 4; nuclear receptor subfamily 3 group A member 2

NCBI Summary for ESR2

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for ESR2

ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: nucleoplasm; mitochondrion; extracellular region; nucleus

Molecular Function: receptor antagonist activity; ligand-dependent nuclear receptor activity; protein binding; estrogen receptor activity; enzyme binding; DNA binding; zinc ion binding; transcription coactivator activity; steroid hormone receptor activity; estrogen response element binding; transcription factor activity; steroid binding

Biological Process: transcription initiation from RNA polymerase II promoter; estrogen receptor signaling pathway; induction of apoptosis by hormones; neuron migration; vagina development; negative regulation of transcription from RNA polymerase II promoter; signal transduction; uterus development; ovarian follicle development; regulation of transcription, DNA-dependent; cell-cell signaling; positive regulation of transcription factor activity; steroid hormone mediated signaling; gene expression; negative regulation of cell growth; brain development; negative regulation of epithelial cell proliferation
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Research Articles on ESR2

1. Data indicate that carnosol could significantly increase estrogen receptors ERalpha and ERbeta expressions of T47D cells, and remarkably increase ERalpha/ERbeta ratio.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ESR2 antibody

 Products by Pathway  Pathway Diagram
 Estrogen Signaling Pathway antibodies  Estrogen Signaling Pathway Diagram
 Estrogen Signaling Pathway antibodies  Estrogen Signaling Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
 Nuclear Receptors Pathway antibodies  Nuclear Receptors Pathway Diagram
 Ovarian Infertility Genes Pathway antibodies  Ovarian Infertility Genes Pathway Diagram
 Plasma Membrane Estrogen Receptor Signaling Pathway antibodies  Plasma Membrane Estrogen Receptor Signaling Pathway Diagram
 Prolactin Signaling Pathway antibodies  Prolactin Signaling Pathway Diagram

Diseases associated with anti-ESR2 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >32 publications with ESR2 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >30 publications with ESR2 and Cardiovascular Diseases
 Genital Diseases, Male Antibodies  >27 publications with ESR2 and Genital Diseases, Male
 Colorectal Neoplasms Antibodies  >15 publications with ESR2 and Colorectal Neoplasms
 Endometriosis Antibodies  >14 publications with ESR2 and Endometriosis
 Infertility, Male Antibodies  >14 publications with ESR2 and Infertility, Male
 Infertility, Female Antibodies  >12 publications with ESR2 and Infertility, Female
 Prostatic Neoplasms Antibodies  >11 publications with ESR2 and Prostatic Neoplasms
 Hypertension Antibodies  >10 publications with ESR2 and Hypertension
 Carcinoma Antibodies  >10 publications with ESR2 and Carcinoma

Organs/Tissues associated with anti-ESR2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >43 publications with ESR2 and Brain
 Testis Antibodies  >31 publications with ESR2 and Testis
 Lung Antibodies  >5 publications with ESR2 and Lung
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