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CD83 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS219348

Rat anti Mouse CD83 antibody, clone 3D11 recognizes murine CD83, a 45kD type 1 glyoprotein belonging to the immunoglobulin superfamily. Originally described as a maturation marker for dendritic cells, CD83 plays a role in the regulation of antigen… More details

$510 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD83, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD83:FITC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3D11
Host
Rat
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from the date of despatch.
Molecular Weight
21,313 Da
3D Structure
ModBase 3D Structure for O88324
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners
Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 mouse myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD83 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD83 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-CD83 antibody

Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83 (MBS217378)
Rat Anti-CD83 Monoclonal Antibody – Cat# MBS219348

Testing Data of anti-CD83 antibody

Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:Alexa Fluor 488 (MBS217378A488)
Rat Anti-CD83 Monoclonal Antibody, Testing Data – Cat# MBS219348

Testing Data of anti-CD83 antibody

Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83 (MBS217379)
Rat Anti-CD83 Monoclonal Antibody, Testing Data – Cat# MBS219348

Testing Data of anti-CD83 antibody

Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:FITC
Rat Anti-CD83 Monoclonal Antibody, Testing Data – Cat# MBS219348

Testing Data of anti-CD83 antibody

Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:Alexa Fluor 647 (MBS217378A647)
Rat Anti-CD83 Monoclonal Antibody, Testing Data – Cat# MBS219348

Testing Data of anti-CD83 antibody

Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:RPE (MBS219486)
Rat Anti-CD83 Monoclonal Antibody, Testing Data – Cat# MBS219348

Other Notes

Small volumes of anti-CD83 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD83 purchase

MBS219348 is an antibody targeting CD83. Also known as RAT ANTI MOUSE CD83:FITC. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD83. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD83 antigen isoform 1
NCBI Official Synonym Full Names
CD83 antigen
NCBI Official Symbol
Cd83
UniProt Protein Name
CD83 antigen
UniProt Synonym Protein Names
CD_antigen: CD83
Protein Family
UniProt Gene Name
Cd83
UniProt Synonym Gene Names
mCD83
UniProt Entry Name
CD83_MOUSE
NCBI Protein Information
CD83 antigen; mCD83

UniProt Comments for CD83

CD83: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Biological Process: negative regulation of interleukin-4 production; positive regulation of interleukin-2 production; positive regulation of CD4-positive, alpha beta T cell differentiation; response to organic cyclic substance; positive regulation of interleukin-10 production
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD83 antibody

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