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F4/80 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS219371

Description: Rat anti Mouse F4/80 antibody, clone A3-1 recognizes the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like… More details

$700 / 2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
F4/80, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE F4/80
Product Gene Name
anti-F4/80 antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
Cl:A3-1
Host
Rat
Form/Format
Tissue Culture Supernatant - liquid
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Immunogen
Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Fusion Partners
Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-F4/80 antibody
Flow Cytometry (FC), Immunohistology Frozen, Immunohistology Paraffin*, Immunohistology Resin (RE), Immunoprecipitation (IP), Immunofluorescence (IF), Radioimmunoassays (RIA), Immunoelectron Microscopy (EM)
Application Notes for anti-F4/80 antibody
FC: Neat- 1/10
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls
1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.

Testing Data of anti-F4/80 antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC (MBS216414)
Rat Anti-F4/80 Monoclonal Antibody – Cat# MBS219371

Testing Data of anti-F4/80 antibody

Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 (MBS216424), red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS219371

Testing Data of anti-F4/80 antibody

Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC (MBS216408)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS219371

Testing Data of anti-F4/80 antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750 (MBS219371P750)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS219371

Testing Data of anti-F4/80 antibody

Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin (MBS216412)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS219371

Testing Data of anti-F4/80 antibody

Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP (MBS235195) or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic MBS219371 (HCA154) followed by Goat anti Rat IgG:HRP (MBS235195)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS219371

Testing Data of anti-F4/80 antibody

Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin (MBS216409)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS219371

Testing Data of anti-F4/80 antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue(MBS219371PB)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS219371

Testing Data of anti-F4/80 antibody

Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS219371

Testing Data of anti-F4/80 antibody

Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin (MBS216410)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS219371

Other Notes

Small volumes of anti-F4/80 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F4/80 purchase

MBS219371 is an antibody targeting F4/80. Also known as RAT ANTI MOUSE F4/80. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for F4/80. It may not necessarily be applicable to this product.

NCBI GI #
33859546
NCBI GeneID
13733
NCBI Accession #
NP_034260.1
UniProt Related Accession #
Q61549
GenBank Nucleotide #
NM_010130.4
NCBI Official Full Name
EGF-like module-containing mucin-like hormone receptor-like 1
NCBI Official Synonym Full Names
EGF-like module containing, mucin-like, hormone receptor-like sequence 1
NCBI Official Symbol
Emr1
NCBI Official Synonym Symbols
Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
UniProt Protein Name
EGF-like module-containing mucin-like hormone receptor-like 1
UniProt Synonym Protein Names
Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 hormone receptor
UniProt Gene Name
Emr1
UniProt Synonym Gene Names
Gpf480
UniProt Entry Name
EMR1_MOUSE
NCBI Protein Information
EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71

UniProt Comments for F4/80

EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 2; Membrane protein, integral; Receptor, GPCR

Cellular Component: membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction
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Research Articles on F4/80

1. EMR1 expression is restricted to mature blood and tissue eosinophils. Targeting of eosinophils with afucosylated anti-EMR1 antibody shows promise as a treatment for eosinophilic disorders.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-F4/80 antibody

 Products by Pathway  Pathway Diagram
 GPCRs, Class B Secretin-like Pathway antibodies  GPCRs, Class B Secretin-like Pathway Diagram
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Diseases associated with anti-F4/80 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >31 publications with F4/80 and Inflammation
 Insulin Resistance Antibodies  >15 publications with F4/80 and Insulin Resistance
 Necrosis Antibodies  >8 publications with F4/80 and Necrosis
 Liver Diseases Antibodies  >7 publications with F4/80 and Liver Diseases
 Fibrosis Antibodies  >6 publications with F4/80 and Fibrosis
 Fatty Liver Antibodies  >6 publications with F4/80 and Fatty Liver
 Cardiovascular Diseases Antibodies  >4 publications with F4/80 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >3 publications with F4/80 and Atherosclerosis
 Weight Gain Antibodies  >3 publications with F4/80 and Weight Gain
 Weight Loss Antibodies  >2 publications with F4/80 and Weight Loss

Organs/Tissues associated with anti-F4/80 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >31 publications with F4/80 and Blood
 Connective Tissue Antibodies  >15 publications with F4/80 and Connective Tissue
 Liver Antibodies  >12 publications with F4/80 and Liver
 Muscle Antibodies  >6 publications with F4/80 and Muscle
 Spleen Antibodies  >4 publications with F4/80 and Spleen
 Bone Antibodies  >3 publications with F4/80 and Bone
 Bone Marrow Antibodies  >3 publications with F4/80 and Bone Marrow
 Embryonic Tissue Antibodies  >2 publications with F4/80 and Embryonic Tissue
 Kidney Antibodies  >2 publications with F4/80 and Kidney
 Lung Antibodies  >2 publications with F4/80 and Lung
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