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CD4 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS219398

MBS219398 is an antibody targeting CD4. Also known as RAT ANTI MOUSE CD4:Low Endotoxin. Host: Rat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD4, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD4:Low Endotoxin
Product Gene Name
anti-CD4 antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
GK1.5
Host
Rat
Form/Format
Low Endotoxin
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
24,473 Da
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Murine CD4
Fusion Partners||Spleen cells from immunised Lewis rats were fused with cells of the SP2/0 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD4 antibody
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-CD4 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200

Testing Data of anti-CD4 antibody

Staining of mouse spleen with Rat anti Mouse CD4 (MBS219566)
Rat Anti-CD4 Monoclonal Antibody – Cat# MBS219398

Testing Data of anti-CD4 antibody

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Medium power
Rat Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS219398

Testing Data of anti-CD4 antibody

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). High power
Rat Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS219398

Testing Data of anti-CD4 antibody

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Low power
Rat Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS219398

Testing Data of anti-CD4 antibody

Published customer image: Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From: Zhou et al. BMC Immunology 2011 12:7.
Rat Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS219398

Testing Data of anti-CD4 antibody

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4 Alexa Fluor ?647 (MBS219566A647)
Rat Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS219398

Testing Data of anti-CD4 antibody

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC (MBS219462)
Rat Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS219398

Testing Data of anti-CD4 antibody

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:Low Endotoxin
Rat Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS219398

Testing Data of anti-CD4 antibody

Staining of mouse spleen with Rat anti Mouse CD4:Alexa Fluor ?488 (MBS219566A488)
Rat Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS219398

Testing Data of anti-CD4 antibody

Staining of mouse spleen with Rat anti Mouse CD4:RPE (MBS219761)
Rat Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS219398

Other Notes

Small volumes of anti-CD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD4. It may not necessarily be applicable to this product.

NCBI GI #
7304953
NCBI GeneID
12504
NCBI Accession #
NP_038516.1
UniProt Related Accession #
P06332
GenBank Nucleotide #
NM_013488.2
NCBI Official Full Name
T-cell surface glycoprotein CD4
NCBI Official Synonym Full Names
CD4 antigen
NCBI Official Symbol
Cd4
NCBI Official Synonym Symbols
L3T4; Ly-4
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Synonym Protein Names
T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; CD_antigen: CD4
Protein Family
T-cell surface glycoprotein
UniProt Gene Name
Cd4
UniProt Entry Name
CD4_MOUSE
NCBI Protein Information
T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

UniProt Comments for CD4

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: endoplasmic reticulum membrane; cell surface; membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; enzyme binding; protein homodimerization activity; zinc ion binding; coreceptor activity; glycoprotein binding; protein kinase binding

Biological Process: maintenance of cellular protein localization; T cell activation; immune system process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; induction by virus of cell-cell fusion in host; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; immune response; positive regulation of T cell activation; cell adhesion; T cell differentiation; regulation of T cell activation
Show more

Research Articles on CD4

1. This study establishes an important role of IgE in abdominal aortic aneurysms pathogenesis by activating CD4+ T cells, mast cells, and macrophages.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD4 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alpha-defensins Pathway antibodies  Alpha-defensins Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
 Defensins Pathway antibodies  Defensins Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram

Diseases associated with anti-CD4 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >12218 publications with CD4 and Inflammation
 Disease Models, Animal Antibodies  >9067 publications with CD4 and Disease Models, Animal
 Necrosis Antibodies  >7648 publications with CD4 and Necrosis
 Lung Diseases Antibodies  >6768 publications with CD4 and Lung Diseases
 Liver Diseases Antibodies  >4684 publications with CD4 and Liver Diseases
 AIDS-Related Opportunistic Infections Antibodies  >4364 publications with CD4 and AIDS-Related Opportunistic Infections
 Carcinoma Antibodies  >3428 publications with CD4 and Carcinoma
 Cardiovascular Diseases Antibodies  >3187 publications with CD4 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >2980 publications with CD4 and Neoplasms, Experimental
 Vascular Diseases Antibodies  >2496 publications with CD4 and Vascular Diseases

Organs/Tissues associated with anti-CD4 antibody

 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >9465 publications with CD4 and Spleen
 Thymus Antibodies  >7207 publications with CD4 and Thymus
 Mixed Antibodies  >3838 publications with CD4 and Mixed
 Brain Antibodies  >3322 publications with CD4 and Brain
 Salivary Gland Antibodies  >424 publications with CD4 and Salivary Gland
 Mammary Gland Antibodies  >137 publications with CD4 and Mammary Gland
 Pineal Gland Antibodies  >33 publications with CD4 and Pineal Gland
 Uncharacterized Tissue Antibodies  >20 publications with CD4 and Uncharacterized Tissue
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