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Tartrate Resistant Acid Phosphatase 5b (TRACP-5B) ELISA Kit for Sheep

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS021967

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of TRACP-5B (hereafter termed "analyte") in undiluted… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Tartrate Resistant Acid Phosphatase 5B (TRACP-5B), ELISA Kit
Full Product Name
Sheep Tartrate Resistant Acid Phosphatase 5B ELISA Kit
Product Gene Name
TRACP-5B elisa kit
Species Reactivity
Sheep
Assay Type
Quantitative Sandwich
Detection Range
0.5U/L-16U/L
Sensitivity
0.1U/L
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
36,599 Da
OMIM
171640
3D Structure
ModBase 3D Structure for P13686

Other Notes

Small volumes of TRACP-5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRACP-5B purchase

MBS021967 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tartrate Resistant Acid Phosphatase 5B (TRACP-5B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRACP-5B. The ELISA analytical biochemical technique of the MBS021967 kit is based on TRACP-5B antibody-TRACP-5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRACP-5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRACP-5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TRACP-5B. It may not necessarily be applicable to this product.

NCBI GI #
161377455
NCBI GeneID
54
NCBI Accession #
NP_001104506.1
UniProt Primary Accession #
P13686
UniProt Secondary Accession #
Q2TAB1; Q6IAS6; Q9UCJ5; Q9UCJ6; Q9UCJ7; A8K3V2
UniProt Related Accession #
P13686
GenBank Nucleotide #
NM_001111036.1
NCBI Official Full Name
tartrate-resistant acid phosphatase type 5
NCBI Official Synonym Full Names
acid phosphatase 5, tartrate resistant
NCBI Official Symbol
ACP5
NCBI Official Synonym Symbols
TRAP; SPENCDI
UniProt Protein Name
Tartrate-resistant acid phosphatase type 5
UniProt Synonym Protein Names
Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase
UniProt Gene Name
ACP5
UniProt Synonym Gene Names
TR-AP; TrATPase
UniProt Entry Name
PPA5_HUMAN
NCBI Protein Information
tartrate-resistant acid phosphatase type 5; TrATPase; tartrate-resistant acid ATPase

NCBI Summary for TRACP-5B

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

UniProt Comments for TRACP-5B

ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Protein type: Cofactor and Vitamin Metabolism - riboflavin; Phosphatase; EC 3.1.3.2; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; integral to membrane; cytosol

Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding

Biological Process: negative regulation of interleukin-12 production; vitamin metabolic process; negative regulation of nitric oxide biosynthetic process; response to lipopolysaccharide; negative regulation of interleukin-1 beta production; negative regulation of superoxide release; negative regulation of tumor necrosis factor production; riboflavin metabolic process; defense response to Gram-positive bacterium; dephosphorylation; negative regulation of inflammatory response; response to cytokine stimulus; water-soluble vitamin metabolic process; bone resorption

Disease: Spondyloenchondrodysplasia With Immune Dysregulation
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Research Articles on TRACP-5B

1. TRACP5b has limited utility as a single marker of metabolic bone disease treatment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TRACP-5B elisa kit

 Reference Product  PubMed Publications
 CSF1R elisa kit  >7 publications with TRACP-5B and CSF1R
 SPP1 elisa kit  >5 publications with TRACP-5B and SPP1

Pathways associated with TRACP-5B elisa kit

 Products by Pathway  Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
 Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies  Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
 Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies  Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
 Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies  Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
 Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies  Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram
 Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway antibodies  Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway Diagram

Diseases associated with TRACP-5B elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with TRACP-5B and Neoplasms
 Liver Diseases Antibodies  >4 publications with TRACP-5B and Liver Diseases
 Inflammation Antibodies  >3 publications with TRACP-5B and Inflammation
 Necrosis Antibodies  >2 publications with TRACP-5B and Necrosis
 Carcinoma, Hepatocellular Antibodies  >2 publications with TRACP-5B and Carcinoma, Hepatocellular
 Lung Diseases Antibodies  >2 publications with TRACP-5B and Lung Diseases
 Atherosclerosis Antibodies  >2 publications with TRACP-5B and Atherosclerosis
 Spondyloenchondrodysplasia Antibodies  >2 publications with TRACP-5B and Spondyloenchondrodysplasia
 Obesity Antibodies  >2 publications with TRACP-5B and Obesity
 Cardiovascular Diseases Antibodies  >1 publications with TRACP-5B and Cardiovascular Diseases

Organs/Tissues associated with TRACP-5B elisa kit

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >38 publications with TRACP-5B and Bone
 Liver Antibodies  >8 publications with TRACP-5B and Liver
 Blood Antibodies  >8 publications with TRACP-5B and Blood
 Connective Tissue Antibodies  >7 publications with TRACP-5B and Connective Tissue
 Lung Antibodies  >3 publications with TRACP-5B and Lung
 Uterus Antibodies  >2 publications with TRACP-5B and Uterus
 Kidney Antibodies  >2 publications with TRACP-5B and Kidney
 Brain Antibodies  >2 publications with TRACP-5B and Brain
 Eye Antibodies  >1 publications with TRACP-5B and Eye
 Parathyroid Antibodies  >1 publications with TRACP-5B and Parathyroid
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