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CD11b Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS219843

Mouse anti Rat CD11b antibody, clone ED8 recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. It also recognizes small ramified microglia in the central nervous system. No other cell types are positive for ED8. The… More details

$605 / 0.25 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD11b, Monoclonal Antibody
Full Product Name
MOUSE ANTI RAT CD11b
Product Gene Name
anti-CD11b antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ED8
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
102,441 Da
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Rat spleen cell homogenate with FCA
Fusion Partners||Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Rat
Applications Tested/Suitable for anti-CD11b antibody
Immunohistology Frozen*
Application Notes for anti-CD11b antibody
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.

Testing Data of anti-CD11b antibody

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 (MBS210091), red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
Mouse Anti-CD11b Monoclonal Antibody – Cat# MBS219843

Testing Data of anti-CD11b antibody

Published customer image: Lithium inhibits lipopolysaccharide (LPS)-induced microglial activation. Primary microglial cells were pretreated with LiCl at 0.3, 1 and 3 mM for 30 minutes and then stimulated with LPS at 10 ng/ml for 24 hours. (a) and (b) For flow cytometric analysis, the cells were incubated with PE-conjugated ED8 antibody at 37 degree C for 1 hour. **P
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS219843

Testing Data of anti-CD11b antibody

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MBS210091) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS219843

Testing Data of anti-CD11b antibody

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 (MBS210091), red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS219843

Testing Data of anti-CD11b antibody

Published customer image: TLR4 regulates microglial activation. Primary microglial cells were transfected with TLR4 siRNA (100 nM) or control (siControl) for 48 hours and then stimulated with lipopolysaccharide (LPS) (10 ng/ml) for 24 hours. (a) Suppression of TLR4 expression by TLR4 siRNA. (b) Quantification of IL-6 and TNF-a production in media, **P
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS219843

Testing Data of anti-CD11b antibody

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MBS210091) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Low power
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS219843

Testing Data of anti-CD11b antibody

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MBS210091) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS219843

Other Notes

Small volumes of anti-CD11b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD11b purchase

MBS219843 is an antibody targeting CD11b. Also known as MOUSE ANTI RAT CD11b. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD11b. It may not necessarily be applicable to this product.

NCBI GI #
1616950
NCBI GeneID
397459
NCBI Accession #
AAB16869.1
NCBI Official Full Name
CD11b, partial
NCBI Official Symbol
ITGAM
NCBI Official Synonym Symbols
CD11B
UniProt Protein Name
CD11b
UniProt Synonym Protein Names
CD11b
Protein Family
Integrin
UniProt Gene Name
CD11b
UniProt Entry Name
Q28984_PIG
NCBI Protein Information
integrin alpha-M

Research Articles on CD11b

1. molecular cloning, characterization, gene expression and chromosomal localization on region SSC3p15-17

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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