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HISTIDINE Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS219884

Mouse anti penta histidine tag antibody, clone ABD2.2.20, recognizes proteins and peptides containing the H-H-H-H-H or H-H-H-H-H-H motif and is therefore of value in detecting proteins containing 5x (penta) his and 6x (hexa) his tags. This antibody will… More details

$655 / 0.125 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HISTIDINE TAG, Monoclonal Antibody
Full Product Name
MOUSE ANTI PENTA HISTIDINE TAG:HRP
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ABD2.2.20
Form/Format
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Specificity
Mouse anti penta histidine tag antibody, clone ABD2.2.20, recognizes proteins and peptides containing the H-H-H-H-H or H-H-H-H-H-H motif and is therefore of value in detecting proteins containing 5x (penta) his and 6x (hexa) his tags. This antibody will detect any accessible N-terminal, C-terminal or internal histidine tags and is highly recommended for use in assay development for the detection of his-tagged HuCAL primary antibodies.
Preparation and Storage
Store at 4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Target Species
Synthetic Peptide
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilizers
Stabilized solution
Applications Tested/Suitable for anti-HISTIDINE TAG antibody
ELISA, Western Blot (WB)
Application Notes for anti-HISTIDINE TAG antibody
ELISA: 1/1000 - 1/2000
WB: 1/1000 - 1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Other Notes

Small volumes of anti-HISTIDINE TAG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HISTIDINE TAG purchase

MBS219884 is an antibody targeting HISTIDINE TAG. Also known as MOUSE ANTI PENTA HISTIDINE TAG:HRP.
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