Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1/CD31) ELISA Kit for Mouse

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate ZAG concentrations in Mouse serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research!

Small volumes of PECAM-1/CD31 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS022000 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1/CD31) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PECAM-1/CD31. The ELISA analytical biochemical technique of the MBS022000 kit is based on PECAM-1/CD31 antibody-PECAM-1/CD31 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PECAM-1/CD31 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PECAM-1/CD31. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).