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G-CSF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS220117

Rabbit anti Mouse G-CSF antibody recognizes mouse granulocyte colony stimulating factor, also known as G-CSF. G-CSF stimulates the proliferation and differentiation of granulocyte progenitors in the bone marrow. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
G-CSF, Polyclonal Antibody
Full Product Name
RABBIT ANTI MOUSE G-CSF:Biotin
Product Gene Name
anti-G-CSF antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Biotin
Purified IgG conjugated to Biotin - lyophilised
Concentration
IgG concentration 50 ug/ml (lot specific)
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0. 09% sodium azide is recommended for long term storage.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
22,421 Da
3D Structure
ModBase 3D Structure for P09920
Reconstitution
Reconstitute with 1.0 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Immunogen
Recombinant mouse G-CSF
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to mouse G-CSF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Mouse
Applications Tested/Suitable for anti-G-CSF antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-G-CSF antibody
ELISA: Minimum Dilution: 0.15ug/ml; Maximum Dilution: 0.30ug/ml;
Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml

Other Notes

Small volumes of anti-G-CSF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G-CSF purchase

MBS220117 is an antibody targeting G-CSF. Also known as RABBIT ANTI MOUSE G-CSF:Biotin. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for G-CSF. It may not necessarily be applicable to this product.

NCBI GI #
6753536
NCBI GeneID
12985
NCBI Accession #
NP_034101.1
UniProt Primary Accession #
P09920
UniProt Related Accession #
P09920
GenBank Nucleotide #
NM_009971.1
NCBI Official Full Name
granulocyte colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 3 (granulocyte)
NCBI Official Symbol
Csf3
NCBI Official Synonym Symbols
Csfg; G-CSF; MGI-IG
UniProt Protein Name
Granulocyte colony-stimulating factor
Protein Family
Granulocyte colony-stimulating factor receptor
UniProt Gene Name
Csf3
UniProt Synonym Gene Names
Csfg; G-CSF
UniProt Entry Name
CSF3_MOUSE
NCBI Protein Information
granulocyte colony-stimulating factor

UniProt Comments for G-CSF

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: enzyme binding; growth factor activity; cytokine activity

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein binding; positive regulation of actin filament polymerization; positive regulation of myeloid cell differentiation; granulocyte differentiation; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation
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Research Articles on G-CSF

1. Cultured mouse enteric nervous system -neurospheres show increased expansion with increased G-CSF concentrations, in contrast to central nervous system - derived spheres.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-G-CSF antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram

Diseases associated with anti-G-CSF antibody

 Disease Name  Pubmed Publications
 Disease Susceptibility Antibodies  >6 publications with G-CSF and Disease Susceptibility
 Nervous System Diseases Antibodies  >5 publications with G-CSF and Nervous System Diseases
 Breast Neoplasms Antibodies  >3 publications with G-CSF and Breast Neoplasms
 Neutropenia Antibodies  >3 publications with G-CSF and Neutropenia
 Leukopenia Antibodies  >2 publications with G-CSF and Leukopenia
 Anemia Antibodies  >2 publications with G-CSF and Anemia
 Neoplasm Metastasis Antibodies  >2 publications with G-CSF and Neoplasm Metastasis
 Leukemia, Myeloid, Acute Antibodies  >2 publications with G-CSF and Leukemia, Myeloid, Acute
 Peripheral Nervous System Diseases Antibodies  >1 publications with G-CSF and Peripheral Nervous System Diseases
 Pulmonary Fibrosis Antibodies  >1 publications with G-CSF and Pulmonary Fibrosis

Organs/Tissues associated with anti-G-CSF antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >5 publications with G-CSF and Lung
 Mammary Gland Antibodies  >3 publications with G-CSF and Mammary Gland
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