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Il-6 (IL6) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS220146

Rabbit anti Rat Interleukin-6 antibody recognizes rat interleukin-6. IL-6 is a pleiotropic cytokine involved in the regulation of immune and inflammatory responses. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IL-6 (Il6), Polyclonal Antibody
Full Product Name
RABBIT ANTI RAT INTERLEUKIN-6:Biotin
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Biotin
Purified IgG conjugated to Biotin - lyophilised
Concentration
IgG concentration 0.1 mg/ml after reconstitution (lot specific)
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
24,357 Da
3D Structure
ModBase 3D Structure for P20607
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Immunogen
Recombinant Rat IL-6 (PRP21)
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to rat IL-6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Rat
Applications Tested/Suitable for anti-Il6 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-Il6 antibody
ELISA: Anti Rat IL-6: biotin conjugate may be used in a direct ELISA with recombinant rat IL-6 as the standard.
ELISA: Minimum Dilution: 1.0 ug/ml
Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml

Other Notes

Small volumes of anti-Il6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Il6 purchase

MBS220146 is an antibody targeting Il6. Also known as RABBIT ANTI RAT INTERLEUKIN-6:Biotin. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Il6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
UniProt Protein Name
Interleukin-6
Protein Family
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_RAT
NCBI Protein Information
interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)

NCBI Summary for Il6

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

UniProt Comments for Il6

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-Il6 antibody

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