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RANTES (CCL5) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS220147

Rabbit anti Rat RANTES antibody recognizes rat RANTES, also known as CCL5, a chemoattractant for blood monocytes, memory T-helper cells and eosinophils. RANTES causes the release of histamine from basophils and activates eosinophils. Rabbit anti Rat RANTES… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
RANTES (Ccl5), Polyclonal Antibody
Full Product Name
RABBIT ANTI RAT RANTES:Biotin
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Biotin
Purified IgG conjugated to Biotin - lyophilised
Concentration
IgG concentration 50 ug/ml (lot specific)
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0. 09% sodium azide is recommended for long term storage.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
10,170 Da
3D Structure
ModBase 3D Structure for P50231
Reconstitution
Reconstitute with 1.0 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Immunogen
Recombinant rat RANTES
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to rat RANTES were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Rat
Applications Tested/Suitable for anti-Ccl5 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-Ccl5 antibody
ELISA: Minimum Dilution: 0.15ug/ml; Maximum Dilution: 0.30ug/ml;
Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml

Other Notes

Small volumes of anti-Ccl5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ccl5 purchase

MBS220147 is an antibody targeting Ccl5. Also known as RABBIT ANTI RAT RANTES:Biotin. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Ccl5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
C-C motif chemokine 5
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 5
NCBI Official Symbol
Ccl5
NCBI Official Synonym Symbols
Scya5; Rantes
UniProt Protein Name
C-C motif chemokine 5
UniProt Synonym Protein Names
SIS-delta; Small-inducible cytokine A5; T-cell-specific protein RANTES
Protein Family
UniProt Gene Name
Ccl5
UniProt Synonym Gene Names
Scya5
UniProt Entry Name
CCL5_RAT
NCBI Protein Information
C-C motif chemokine 5; SIS-delta; T-cell-specific protein RANTES; regulated upon activation normal T-cell expressed and secreted; small inducible cytokine A5; small-inducible cytokine A5

NCBI Summary for Ccl5

may play a role in cellular response to viral infection in testicular somatic cells [RGD, Feb 2006]

UniProt Comments for Ccl5

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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