Rabbit PAPPA2 ELISA Kit – Cat# MBS022069

Pappalysin 2 (PAPPA2) ELISA Kit for Rabbit

Name: Rabbit PAPPA2 ELISA Kit; Pappalysin 2; MyBioSource
Brand: MyBioSource
SKU: MBS022069
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS022069

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of PAPPA2 (hereafter termed "analyte") in undiluted… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Pappalysin 2 (PAPPA2), ELISA Kit

Full Product Name

Rabbit Pappalysin 2 ELISA Kit

Product Gene Name

PAPPA2 elisa kit

Species Reactivity

Rabbit

Assay Type

Quantitative Sandwich

Detection Range

0.25ng/ml-8ng/ml

Sensitivity

0.1ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Molecular Weight

198,539 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (176432307..176811972). Location: 1q23-q25

3D Structure

ModBase 3D Structure for Q9BXP8

Other Notes

Small volumes of PAPPA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAPPA2 purchase

MBS022069 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pappalysin 2 (PAPPA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PAPPA2. The ELISA analytical biochemical technique of the MBS022069 kit is based on PAPPA2 antibody-PAPPA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAPPA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAPPA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PAPPA2. It may not necessarily be applicable to this product.

NCBI GI #

116174738

NCBI GeneID

60676

NCBI Accession #

NP_064714.2

UniProt Primary Accession #

Q9BXP8

UniProt Secondary Accession #

Q96PH7; Q96PH8; Q9H4C9; A9Z1Y8

UniProt Related Accession #

Q9BXP8

GenBank Nucleotide #

NM_020318.2

NCBI Official Full Name

pappalysin-2 isoform 1

NCBI Official Synonym Full Names

pappalysin 2

NCBI Official Symbol

PAPPA2

NCBI Official Synonym Symbols

PAPPE; PLAC3; PAPP-E; PAPP-A2

UniProt Protein Name

Pappalysin-2

UniProt Synonym Protein Names

Pregnancy-associated plasma protein A2; PAPP-A2; Pregnancy-associated plasma protein E1

Protein Family

Pappalysin

UniProt Gene Name

PAPPA2

UniProt Synonym Gene Names

PLAC3; PAPP-A2; PAPP-E

UniProt Entry Name

PAPP2_HUMAN

NCBI Protein Information

pappalysin-2; placenta-specific 3; pregnancy-associated plasma protein E1; pregnancy-associated plasma preproprotein-A2

NCBI Summary for PAPPA2

This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

UniProt Comments for PAPPA2

PAPPA2: Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. Belongs to the peptidase M43B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted, signal peptide; EC 3.4.24.-; Secreted

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: membrane; extracellular region; intracellular

Molecular Function: zinc ion binding; metallopeptidase activity

Biological Process: cellular protein metabolic process; regulation of cell growth; cell differentiation; proteolysis

Research Articles on PAPPA2

1. Association of a single nucleotide polymorphism in pregnancy-associated plasma protein-A2 with developmental dysplasia of the hip

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PAPPA2 elisa kit

Reference Product	PubMed Publications
IGFBP5 elisa kit	>7 publications with PAPPA2 and IGFBP5
IGFBP4 elisa kit	>2 publications with PAPPA2 and IGFBP4

Pathways associated with PAPPA2 elisa kit

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies	Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram

Diseases associated with PAPPA2 elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>2 publications with PAPPA2 and Necrosis
Myocardial Infarction Antibodies	>1 publications with PAPPA2 and Myocardial Infarction
Anemia Antibodies	>1 publications with PAPPA2 and Anemia
Pain Antibodies	>1 publications with PAPPA2 and Pain
Cardiovascular Diseases Antibodies	>1 publications with PAPPA2 and Cardiovascular Diseases

Organs/Tissues associated with PAPPA2 elisa kit

Organ/Tissue Name	Pubmed Publications
Placenta Antibodies	>11 publications with PAPPA2 and Placenta
Bone Antibodies	>3 publications with PAPPA2 and Bone
Liver Antibodies	>2 publications with PAPPA2 and Liver
Heart Antibodies	>2 publications with PAPPA2 and Heart
Brain Antibodies	>2 publications with PAPPA2 and Brain
Uterus Antibodies	>2 publications with PAPPA2 and Uterus
Muscle Antibodies	>1 publications with PAPPA2 and Muscle
Pancreas Antibodies	>1 publications with PAPPA2 and Pancreas
Kidney Antibodies	>1 publications with PAPPA2 and Kidney