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Chromogranin A (CHGA) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS220745

Sheep anti Human Chromogranin antibodyrecognizes human chromogranin A, a secreted peptide that inhibits glucose-induced insulin release. Chromogranin A is an activator of microglia (Taupenaut et al. 1996) and is notably upregulated and prominantly expressed… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CHROMOGRANIN A (CHGA), Polyclonal Antibody
Full Product Name
SHEEP ANTI HUMAN CHROMOGRANIN A
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Bovine (Expected from Sequence), Chimpanzee (Expected from Sequence), Dog (Expected from Sequence), Horse (Expected from Sequence), Mouse (Expected from Sequence), Pig (Expected from Sequence), Rat (Expected from Sequence)
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 5.0 mg/ml (lot specific)
Specificity
CHROMOGRANIN A
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquot at -20 degree C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
OMIM
118910
3D Structure
ModBase 3D Structure for P10645
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Histology Positive Control Tissue
Human pancreas
Buffer Solution
Phosphate buffered saline
Target Species
Human
Immunogen
Synthetic peptide corresponding to the amino acids 1 - 15 (lpvnspmnkgdtevm) of the native molecule conjugated to Keyhole limpet Haemocyanin.
Species Cross Reactivity
Based on sequence similarity, is expected to react with: Bovine, Chimpanzee, Dog, Horse, Pig, Mouse, Rat.
N.B. Antibody reactivity and working conditions may vary between species. Cross reativity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
Specificity Note
Sheep anti Human Chromogranin antibodyrecognizes human chromogranin A, a secreted peptide that inhibits glucose-induced insulin release. Chromogranin A is an activator of microglia (Taupenaut et al. 1996) and is notably upregulated and prominantly expressed in the senile plaques associated with Alzheimer's disease (Yasuhara et al. 1994).
Applications Tested/Suitable for anti-CHGA antibody
ELISA (EIA), Immunohistology-Paraffin* (IHC-P)
Application Notes for anti-CHGA antibody
Immunohistology - Paraffin: Minimum Dilution: 1/25-1/100

Testing Data #1 of anti-CHGA antibody

Staining of paraffin embedded human pancreas with Sheep anti Human Chromogranin A (MBS220745) following pronase antigen retrieval
Sheep Anti-CHGA Polyclonal Antibody – Cat# MBS220745

Other Notes

Small volumes of anti-CHGA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHGA purchase

MBS220745 is an antibody targeting CHGA. Also known as SHEEP ANTI HUMAN CHROMOGRANIN A. Reactive in Bovine (Expected from Sequence), Chimpanzee (Expected from Sequence), Dog (Expected from Sequence), Horse (Expected from Sequence), Mouse (Expected from Sequence), Pig (Expected from Sequence), Rat (Expected from Sequence). Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for CHGA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chromogranin-A isoform 1 preproprotein
NCBI Official Synonym Full Names
chromogranin A (parathyroid secretory protein 1)
NCBI Official Symbol
CHGA
NCBI Official Synonym Symbols
CGA
UniProt Protein Name
Chromogranin-A
UniProt Synonym Protein Names
Pituitary secretory protein I; SP-I
Protein Family
UniProt Gene Name
CHGA
UniProt Synonym Gene Names
CgA; SP-I
UniProt Entry Name
CMGA_HUMAN
NCBI Protein Information
chromogranin-A; SP-I; betagranin (N-terminal fragment of chromogranin A); catestatin; chromofungin; parathyroid secretory protein 1; pituitary secretory protein I

NCBI Summary for CHGA

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
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UniProt Comments for CHGA

CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells.

Protein type: Vesicle; Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: perinuclear region of cytoplasm; extracellular region; secretory granule

Biological Process: regulation of blood pressure
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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