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COLLAGEN II (COL2A1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS220756

MBS220756 is an antibody targeting COL2A1. Also known as RABBIT ANTI HUMAN COLLAGEN II. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
COLLAGEN II (COL2A1), Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN COLLAGEN II
Product Gene Name
Clonality
Polyclonal
Isotype
Polyclonal IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified (Purified IgG - lyophilised)
Concentration
IgG concentration 0.1 mg/ml after reconstitution (lot specific)
Specificity
COLLAGEN II
This item recognizes human Collagen type II, an extracellular matrix protein found mainly in cartilaginous tissue. The antibody reacts with both native and heat denatured forms and shows minimal cross reactivity with Collagen type I, III, IV and V, as demonstrated by competitive ELISA.
Preparation and Storage
Prior to reconstitution store at +4 degree C.
After reconstitution store at -20 degree C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
141,785 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (48366748..48398285, complement). Location: 12q13.11
OMIM
108300
3D Structure
ModBase 3D Structure for P02458
Immunogen
Human Collagen type II from cartilage
Preparation
Purified IgG prepared by affinity chromatography on immobilized human Collagen type II
Preservative Stabilizers
1% Dextran
1% Mannitol
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% BSA is recommended.
Buffer Solution
Borate buffered saline
Applications Tested/Suitable for anti-COL2A1 antibody
Immunohistology - Frozen (IHC), ELISA (EIA)
Application Notes for anti-COL2A1 antibody
Immunohistology - Frozen: Min Dilution: 1/10
Max Dilution: 1/50

ELISA: Min Dilution: 1/500
Max Dilution: 1/2000

Other Notes

Small volumes of anti-COL2A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COL2A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
collagen alpha-1(II) chain isoform 1
NCBI Official Synonym Full Names
collagen, type II, alpha 1
NCBI Official Symbol
COL2A1
NCBI Official Synonym Symbols
AOM; ANFH; SEDC; COL11A3
UniProt Protein Name
Collagen alpha-1(II) chain
UniProt Synonym Protein Names
Alpha-1 type II collagen
UniProt Gene Name
COL2A1
UniProt Entry Name
CO2A1_HUMAN
NCBI Protein Information
collagen alpha-1(II) chain; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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