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COLLAGEN IV (COL4A1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS220758

MBS220758 is an antibody targeting COL4A1. Also known as RABBIT ANTI HUMAN COLLAGEN IV. Reactive in Human. Host: Rabbit. More details

$725 / 0.5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COLLAGEN IV (COL4A1), Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN COLLAGEN IV
Product Gene Name
Clonality
Polyclonal
Isotype
Polyclonal IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified (Purified IgG - lyophilised)
Specificity
COLLAGEN IV
This item exhibits the following reactivities with Collagen IV and related proteins:

Human collagen IV100%
Human types I, III and V< 10%
Human collagen II<1%

Human plasma proteins do not interfere with collagen binding.

Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type IV collagen forms a two-dimensional reticulum and is a major component of the basal lamina.
Preparation and Storage
Store at -20 degree C only.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
160,615 Da
Chromosome Location
Chromosome: 13; NC_000013.10 (110801310..110959496, complement). Location: 13q34
OMIM
120130
3D Structure
ModBase 3D Structure for P02462
Immunogen
Collagen type IV from human placenta
Preparation
Purified IgG prepared by affinity chromatography on immobilized human collagen type IV
Preservative Stabilizers
Contains mannitol, dextran and salts
Reconstitution
Use 500 ul sterile distilled water.
Applications Tested/Suitable for anti-COL4A1 antibody
Immunohistology - Frozen (IHC), Immunofluorescence (IF), ELISA (EIA), Immunohistology - Paraffin (IHC)
Application Notes for anti-COL4A1 antibody
ELISA: Min Dilution: 1/1.5K
Max Dilution: 1/6K

Immunohistology - Frozen: Min Dilution: 1/10
Max Dilution: 1/50

Immunofluorescence: Min Dilution: 1/10
Max Dilution: 1/50

Immunohistology - Paraffin: Min Dilution: 1/10
Max Dilution: 1/50
Application Note: Pretreatment is required with 1 mg/ml pepsin in 0.5 M acetic acid for two hours at 37 degree C and multiple buffer washes.

Other Notes

Small volumes of anti-COL4A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COL4A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
collagen alpha-1(IV) chain preproprotein
NCBI Official Synonym Full Names
collagen, type IV, alpha 1
NCBI Official Symbol
COL4A1
NCBI Official Synonym Symbols
ICH; HANAC; POREN1; arresten
UniProt Protein Name
Collagen alpha-1(IV) chain
Protein Family
UniProt Gene Name
COL4A1
UniProt Entry Name
CO4A1_HUMAN
NCBI Protein Information
collagen alpha-1(IV) chain; collagen alpha-1(IV) chain; COL4A1 NC1 domain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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