Sheep Anti-14-3-3 PROTEIN Polyclonal Antibody – Cat# MBS221102

14-3-3 PROTEIN Antibody – Sheep Polyclonal

Name: 14-3-3 PROTEIN Antibody; Polyclonal; Sheep; MyBioSource
Brand: MyBioSource
SKU: MBS221102
Price: 385 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221102

MBS221102 recognises the lower molecular weight subunit (26 kD) of protein 14-3-3. The 14-3-3 family consists of 30kD proteins that are involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression,… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

14-3-3 (14-3-3 PROTEIN), Polyclonal Antibody

Full Product Name

SHEEP ANTI HUMAN 14-3-3 PROTEIN

Product Gene Name

anti-14-3-3 PROTEIN antibody

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Form/Format

Purified
Purified Ig - liquid

Concentration

Ig concentration 2.5 mg/ml (lot specific)

Specificity

This item recognises the lower molecular weight subunit (26 kD) of protein 14-3-3.The 14-3-3 family consists of 30kD proteins that are involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

27,764 Da

OMIM

609009

Perservative Stabilisers

0.09% Sodium Azide (NaN3)
Preparation||Purified Ig prepared by affinity chromatography on Protein G

Immunogen

Native, purified from pathogen-free normal human brain.
Buffer Solution||Phosphate buffered saline

Antiserum Preparation

Antisera to 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen.

Target Species

Human

Applications Tested/Suitable for anti-14-3-3 PROTEIN antibody

Western Blot (WB)

Application Notes for anti-14-3-3 PROTEIN antibody

Western Blotting: Minimum Dilution: 1/10; Maximum Dilution: 1/50

Other Notes

Small volumes of anti-14-3-3 PROTEIN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 14-3-3 PROTEIN purchase

MBS221102 is an antibody targeting 14-3-3 PROTEIN. Also known as SHEEP ANTI HUMAN 14-3-3 PROTEIN. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for 14-3-3 PROTEIN. It may not necessarily be applicable to this product.

NCBI GI #

5803227

NCBI GeneID

10971

NCBI Accession #

NP_006817.1

UniProt Secondary Accession #

Q567U5; Q5TZU8; Q9UP48; D6W4Z5

UniProt Related Accession #

P27348

GenBank Nucleotide #

NM_006826.3

NCBI Official Full Name

14-3-3 protein theta

NCBI Official Synonym Full Names

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta

NCBI Official Symbol

YWHAQ

NCBI Official Synonym Symbols

1C5; HS1; 14-3-3

UniProt Protein Name

14-3-3 protein theta

UniProt Synonym Protein Names

14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1

Protein Family

14-3-3-like protein

UniProt Gene Name

YWHAQ

UniProt Entry Name

1433T_HUMAN

NCBI Protein Information

14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

NCBI Summary for 14-3-3 PROTEIN

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

UniProt Comments for 14-3-3 PROTEIN

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; cytoplasm; cytosol

Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding

Biological Process: substantia nigra development; apoptosis; small GTPase mediated signal transduction; negative regulation of transcription, DNA-dependent; protein targeting

Research Articles on 14-3-3 PROTEIN

1. CCL20 and 14-3-3 zeta are molecules that play a putative role during tumorgenesis in pancreas, and may therefore be new parameters for histological diagnosis and discrimination between pancreatic neoplasms and chronic pancreatitis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-14-3-3 PROTEIN antibody

Reference Product	PubMed Publications
YWHAB antibody	>1 publications with 14-3-3 PROTEIN and YWHAB
YWHAH antibody	>1 publications with 14-3-3 PROTEIN and YWHAH
BAD antibody	>1 publications with 14-3-3 PROTEIN and BAD

Pathways associated with anti-14-3-3 PROTEIN antibody

Products by Pathway	Pathway Diagram
Activation Of BAD And Translocation To Mitochondria Pathway antibodies	Activation Of BAD And Translocation To Mitochondria Pathway Diagram
Activation Of BH3-only Proteins Pathway antibodies	Activation Of BH3-only Proteins Pathway Diagram
Alpha6-Beta4 Integrin Signaling Pathway antibodies	Alpha6-Beta4 Integrin Signaling Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Calcium Regulation In The Cardiac Cell Pathway antibodies	Calcium Regulation In The Cardiac Cell Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Class I PI3K Signaling Events Mediated By Akt Pathway antibodies	Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram

Diseases associated with anti-14-3-3 PROTEIN antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>1 publications with 14-3-3 PROTEIN and Adenocarcinoma
Breast Neoplasms Antibodies	>1 publications with 14-3-3 PROTEIN and Breast Neoplasms

Organs/Tissues associated with anti-14-3-3 PROTEIN antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>4 publications with 14-3-3 PROTEIN and Muscle
Brain Antibodies	>4 publications with 14-3-3 PROTEIN and Brain
Nerve Antibodies	>3 publications with 14-3-3 PROTEIN and Nerve
Heart Antibodies	>2 publications with 14-3-3 PROTEIN and Heart
Prostate Antibodies	>2 publications with 14-3-3 PROTEIN and Prostate
Blood Antibodies	>2 publications with 14-3-3 PROTEIN and Blood
Embryonic Tissue Antibodies	>2 publications with 14-3-3 PROTEIN and Embryonic Tissue
Liver Antibodies	>1 publications with 14-3-3 PROTEIN and Liver
Kidney Antibodies	>1 publications with 14-3-3 PROTEIN and Kidney
Placenta Antibodies	>1 publications with 14-3-3 PROTEIN and Placenta