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Tryptophan Hydroxylase (TPH1) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221196

MBS221196 recognises tryptophan hydroxylase, an ensyme involved in the synthesis of the neurotransmitter serotonin. In a Western blot Rat pineal, hypothalamus and dorsal raphe tissue have been used. A band at approximately 55 kD is observed. More details

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Product Overview

Product Name
TRYPTOPHAN HYDROXYLASE (TPH1), Polyclonal Antibody
Full Product Name
SHEEP ANTI RABBIT TRYPTOPHAN HYDROXYLASE
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Human, Mammals (Expected from Sequence), Rat
Form/Format
Purified
Purified Ig - liquid
Specificity
This item recognises tryptophan hydroxylase, an ensyme involved in the synthesis of the neurotransmitter serotonin. In a Western blot Rat pineal, hypothalamus and dorsal raphe tissue have been used. A band at approximately 55 kD is observed.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
51,118 Da
3D Structure
ModBase 3D Structure for P17290
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
50% Glycerol
0.01% Bovine Serum Albumin
Preparation||Purified Ig prepared by affinity chromatography
Immunogen
Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E Coli and purified by preparative SDS-PAGE.
Buffer Solution||Phosphate buffered saline
Target Species
Rabbit
Applications Tested/Suitable for anti-TPH1 antibody
Immunohistology Frozen, Western Blot (WB)
Application Notes for anti-TPH1 antibody
Immunohistology: This item has been used for immunohistochemistry on formaldehyde fixed tissue, it is useful for cell body but not fiber staining

Other Notes

Small volumes of anti-TPH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPH1 purchase

MBS221196 is an antibody targeting TPH1. Also known as SHEEP ANTI RABBIT TRYPTOPHAN HYDROXYLASE. Reactive in Human, Mammals (Expected from Sequence), Rat. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for TPH1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
tryptophan 5-hydroxylase 1
NCBI Official Symbol
TPH1
NCBI Official Synonym Symbols
TPH2
UniProt Protein Name
Tryptophan 5-hydroxylase 1
UniProt Synonym Protein Names
Tryptophan 5-monooxygenase 1
UniProt Gene Name
TPH1
UniProt Synonym Gene Names
TPH
UniProt Entry Name
TPH1_RABIT
NCBI Protein Information
tryptophan 5-hydroxylase 1; tryptophan hydroxylase 2

UniProt Comments for TPH1

TPH1: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described.

Protein type: EC 1.14.16.4; Amino Acid Metabolism - tryptophan; Oxidoreductase
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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