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TRIAD3A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221309

MBS221309 recognises an epitope within the N-terminal region (NT) of mouse TRIAD3A, a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response. Regulation of the level and duration of Toll-like receptor… More details

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Product Overview

Product Name
TRIAD3A, Polyclonal Antibody
Full Product Name
RABBIT ANTI MOUSE TRIAD3A (N-TERMINAL)
Product Gene Name
anti-TRIAD3A antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
This item recognises an epitope within the N-terminal region (NT) of mouse TRIAD3A, a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response.

Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
83,612 Da
3D Structure
ModBase 3D Structure for P58283
Perservative Stabilisers
0.02% Sodium Azide
Immunogen
A 15 amino acid peptide located near mouse TRIAD3A amino-terminus.
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to mouse TRIAD3A (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Target Species
Mouse
Applications Tested/Suitable for anti-TRIAD3A antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-TRIAD3A antibody
Western Blot: This item detects a band of approximately 95kDa in mouse heart cell lysates.
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml

Testing Data #1 of anti-TRIAD3A antibody

Western blot analysis of mouse heart lysate probed with Rabbit anti Mouse TRIAD3A (MBS221309) at 0.5(A), 1(B) and 2(C) ug/ml
Rabbit Anti-TRIAD3A Polyclonal Antibody – Cat# MBS221309

Testing Data #2 of anti-TRIAD3A antibody

Formalin fixed, paraffin embedded mouse heart stained with Rabbit anti Mouse TRIAD3A (MBS221309) following antigen retrieval using sodium citrate buffer (pH6.0)
Rabbit Anti-TRIAD3A Polyclonal Antibody, Testing Data #2 – Cat# MBS221309

Other Notes

Small volumes of anti-TRIAD3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIAD3A purchase

MBS221309 is an antibody targeting TRIAD3A. Also known as RABBIT ANTI MOUSE TRIAD3A (N-TERMINAL). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIAD3A. It may not necessarily be applicable to this product.

NCBI GI #
46358407
NCBI GeneID
108086
NCBI Accession #
NP_542128.2
UniProt Primary Accession #
P58283
UniProt Secondary Accession #
Q3U493; Q3UE56; Q3UGM3; Q68FN0; Q6P1H8; Q6PWY5; Q8BN27; Q8C1U3
UniProt Related Accession #
P58283
GenBank Nucleotide #
NM_080561.4
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF216 isoform A
NCBI Official Synonym Full Names
ring finger protein 216
NCBI Official Symbol
Rnf216
NCBI Official Synonym Symbols
UIP83; C86502; TRIAD3; AI647468; AU019462; Ubce7ip1; 2810055G22Rik; F830018F18Rik
UniProt Protein Name
E3 ubiquitin-protein ligase RNF216
UniProt Synonym Protein Names
RING finger protein 216; Triad domain-containing protein 3; UbcM4-interacting protein 83; Ubiquitin-conjugating enzyme 7-interacting protein 1
UniProt Gene Name
Rnf216
UniProt Synonym Gene Names
Triad3; Ubce7ip1; Uip83; Zin
UniProt Entry Name
RN216_MOUSE
NCBI Protein Information
E3 ubiquitin-protein ligase RNF216; E3 ubiquitin ligase; triad domain-containing protein 3; ubcM4-interacting protein 83; ubiquitin-conjugating enzyme 7-interacting protein 1

UniProt Comments for TRIAD3A

TRIAD3: Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down- regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3/ZIN inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; EC 6.3.2.-

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; apoptosis; regulation of interferon-beta production; regulation of defense response to virus by host
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TRIAD3A antibody

 Reference Product  PubMed Publications
 Tirap antibody  >2 publications with TRIAD3A and Tirap
 Ripk1 antibody  >2 publications with TRIAD3A and Ripk1
 Traf3 antibody  >1 publications with TRIAD3A and Traf3

Pathways associated with anti-TRIAD3A antibody

 Products by Pathway  Pathway Diagram
 TNF-alpha NF-kB Signaling Pathway antibodies  TNF-alpha NF-kB Signaling Pathway Diagram

Diseases associated with anti-TRIAD3A antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >5 publications with TRIAD3A and Necrosis
 Nervous System Diseases Antibodies  >3 publications with TRIAD3A and Nervous System Diseases
 Weight Gain Antibodies  >1 publications with TRIAD3A and Weight Gain
 Neoplasm Invasiveness Antibodies  >1 publications with TRIAD3A and Neoplasm Invasiveness
 Ovarian Diseases Antibodies  >1 publications with TRIAD3A and Ovarian Diseases
 Liver Diseases Antibodies  >1 publications with TRIAD3A and Liver Diseases
 Prostatic Diseases Antibodies  >1 publications with TRIAD3A and Prostatic Diseases
 Weight Loss Antibodies  >1 publications with TRIAD3A and Weight Loss
 Immune System Diseases Antibodies  >1 publications with TRIAD3A and Immune System Diseases
 Prenatal Injuries Antibodies  >1 publications with TRIAD3A and Prenatal Injuries

Organs/Tissues associated with anti-TRIAD3A antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with TRIAD3A and Blood
 Brain Antibodies  >2 publications with TRIAD3A and Brain
 Kidney Antibodies  >1 publications with TRIAD3A and Kidney
 Eye Antibodies  >1 publications with TRIAD3A and Eye
 Bone Marrow Antibodies  >1 publications with TRIAD3A and Bone Marrow
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