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NOXA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221324

MBS221324 recognises murine Noxa, an 11kDa member of the BH3-only proapoptotic protein family. Noxa is localised to the mitochondria and is a transcriptional target of p73, p53 and BH3-only proteins. It is thought to induce apoptosis when activated, lowering… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
NOXA, Polyclonal Antibody
Full Product Name
RABBIT ANTI NOXA
Product Gene Name
anti-NOXA antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5mg/ml (lot specific)
Specificity
This item recognises murine Noxa, an 11kDa member of the BH3-only proapoptotic protein family. Noxa is localised to the mitochondria and is a transcriptional target of p73, p53 and BH3-only proteins. It is thought to induce apoptosis when activated, lowering the activity of anti-apoptotic Bcl-2-like proteins. It can be upregulated by mitogenic activation, independently of p53. In humans, along with Mcl-1 it is thought to form a selective pathway that restrains lymphocyte proliferation.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
11,566 Da
3D Structure
ModBase 3D Structure for Q9JM54
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to N-terminus of mouse NOXA.
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to NOXA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Mouse
Applications Tested/Suitable for anti-NOXA antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-NOXA antibody
Western Blot: This item detects a band of approximately 11kDa in human stomach cell lysates.
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml

Testing Data #1 of anti-NOXA antibody

Western blot analysis of human stomach tissue lysate probed with Rabbit anti NOXA (MBS221324) at 0.5(A), 1(B) and 2(C) ug/ml
Rabbit Anti-NOXA Polyclonal Antibody – Cat# MBS221324

Testing Data #2 of anti-NOXA antibody

Formalin fixed, paraffin embedded human stomach stained with Rabbit anti Human NOXA (MBS221324) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Rabbit Anti-NOXA Polyclonal Antibody, Testing Data #2 – Cat# MBS221324

Other Notes

Small volumes of anti-NOXA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOXA purchase

MBS221324 is an antibody targeting NOXA. Also known as RABBIT ANTI NOXA. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NOXA. It may not necessarily be applicable to this product.

NCBI GI #
10946832
NCBI GeneID
58801
NCBI Accession #
NP_067426.1
UniProt Primary Accession #
Q9JM54
UniProt Related Accession #
Q9JM54
GenBank Nucleotide #
NM_021451.2
NCBI Official Full Name
phorbol-12-myristate-13-acetate-induced protein 1
NCBI Official Synonym Full Names
phorbol-12-myristate-13-acetate-induced protein 1
NCBI Official Symbol
Pmaip1
NCBI Official Synonym Symbols
Noxa
UniProt Protein Name
Phorbol-12-myristate-13-acetate-induced protein 1
UniProt Synonym Protein Names
Protein Noxa
Protein Family
Superoxide-generating NADPH oxidase
UniProt Gene Name
Pmaip1
UniProt Synonym Gene Names
Noxa
UniProt Entry Name
APR_MOUSE
NCBI Protein Information
phorbol-12-myristate-13-acetate-induced protein 1; protein Noxa

UniProt Comments for NOXA

NOXA: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. Interacts with MCL1, BCL2A1 and BAX. Up-regulated by p53/TP53, phorbol esters, double- stranded RNA, IFNB1/IFN-beta and viruses. Highly expressed in adult T-cell leukemia cell line. Belongs to the PMAIP1 family.

Protein type: Apoptosis; Mitochondrial

Cellular Component: mitochondrion; intracellular

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; regulation of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of fibroblast proliferation; positive regulation of neuron apoptosis; T cell homeostasis; positive regulation of DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to DNA damage stimulus; response to X-ray; response to UV
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Research Articles on NOXA

1. the current findings indicate that Noxa is a novel regulator of early mitosis before 75% epiboly stage when it translates into a key mediator of apoptosis in subsequent embryogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NOXA antibody

 Reference Product  PubMed Publications
 Bax antibody  >103 publications with NOXA and Bax
 Trp53 antibody  >76 publications with NOXA and Trp53
 Bcl2l1 antibody  >49 publications with NOXA and Bcl2l1
 Mcl1 antibody  >46 publications with NOXA and Mcl1
 Bak1 antibody  >29 publications with NOXA and Bak1
 Bcl2l2 antibody  >4 publications with NOXA and Bcl2l2
 Max antibody  >2 publications with NOXA and Max

Pathways associated with anti-NOXA antibody

 Products by Pathway  Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of NOXA And Translocation To Mitochondria Pathway antibodies  Activation Of NOXA And Translocation To Mitochondria Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway antibodies  BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway Diagram
 Intrinsic Pathway For Apoptosis antibodies  Intrinsic Pathway For Apoptosis Diagram
 Programmed Cell Death Pathway antibodies  Programmed Cell Death Pathway Diagram
 Viral Carcinogenesis Pathway antibodies  Viral Carcinogenesis Pathway Diagram
 Viral Carcinogenesis Pathway antibodies  Viral Carcinogenesis Pathway Diagram
 MiRNA Regulation Of DNA Damage Response Pathway antibodies  MiRNA Regulation Of DNA Damage Response Pathway Diagram

Diseases associated with anti-NOXA antibody

 Disease Name  Pubmed Publications
 Melanoma Antibodies  >31 publications with NOXA and Melanoma
 Leukemia, Lymphocytic, Chronic, B-Cell Antibodies  >28 publications with NOXA and Leukemia, Lymphocytic, Chronic, B-Cell
 Necrosis Antibodies  >23 publications with NOXA and Necrosis
 Breast Neoplasms Antibodies  >21 publications with NOXA and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >21 publications with NOXA and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >20 publications with NOXA and Lung Neoplasms
 Multiple Myeloma Antibodies  >19 publications with NOXA and Multiple Myeloma
 Nervous System Diseases Antibodies  >19 publications with NOXA and Nervous System Diseases
 Lung Diseases Antibodies  >15 publications with NOXA and Lung Diseases
 Inflammation Antibodies  >13 publications with NOXA and Inflammation

Organs/Tissues associated with anti-NOXA antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >63 publications with NOXA and Blood
 Lung Antibodies  >28 publications with NOXA and Lung
 Brain Antibodies  >18 publications with NOXA and Brain
 Bone Antibodies  >16 publications with NOXA and Bone
 Skin Antibodies  >14 publications with NOXA and Skin
 Bone Marrow Antibodies  >11 publications with NOXA and Bone Marrow
 Embryonic Tissue Antibodies  >9 publications with NOXA and Embryonic Tissue
 Prostate Antibodies  >8 publications with NOXA and Prostate
 Stomach Antibodies  >6 publications with NOXA and Stomach
 Kidney Antibodies  >6 publications with NOXA and Kidney
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