Rabbit Anti-BAG1 Polyclonal Antibody – Cat# MBS221459

Western blot analysis in whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 (MBS221459) at 1(A) and 2(B) ug/ml

BAG1 Antibody – Rabbit Polyclonal

Name: BAG1 Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS221459
Price: 740 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221459

MBS221459 detects an epitope within the C-terminal (CT) region of Bcl-2 associated athanogene-1 (BAG1), a co-chaperone protein which binds to and regulates the 70kD heat shock proteins (Hsp70/Hsc70). BAG1 has also been shown to interact with the kinase Raf1,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

BAG1, Polyclonal Antibody

Full Product Name

RABBIT ANTI BAG1 (C-TERMINAL)

Product Gene Name

anti-BAG1 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse, Rat

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 1.0mg/ml (lot specific)

Specificity

This item detects an epitope within the C-terminal (CT) region of Bcl-2 associated athanogene-1 (BAG1), a co-chaperone protein which binds to and regulates the 70kD heat shock proteins (Hsp70/Hsc70). BAG1 has also been shown to interact with the kinase Raf1, a number of steroid and tyrosine kinase receptors, and the Bcl-2 family of proteins. It is thought to function as a molecular switch between cell growth and differentiation, and cell survival.

The BAG1 gene encodes three different isoforms; a predominantly cytosolic form of 36kD (BAG1S), a 46kD cytosolic and nuclear form (BAG1M), and a predominantly nuclear form 50kD (BAG1L).

BAG1 displays potent neuroprotective activity in vivo against stroke, and could be used for reducing brain injury during cerebral ischemia and neurodegenerative diseases. BAG1 may also promote chemoresistance, growth factor independence, and anchorage independent cell growth in tumour cells.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

25,989 Da

OMIM

601497

3D Structure

ModBase 3D Structure for Q99933

Perservative Stabilisers

0.02% Sodium Azide

Immunogen

14 amino acid peptide from near the carboxy terminus of human BAG1.
Buffer Solution||Phosphate buffered saline

Antiserum Preparation

Antisera to BAG1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-BAG1 antibody

Western Blot (WB)

Application Notes for anti-BAG1 antibody

Western Blot: This item detects a band of approximately 39kDa in PC-3 cell lysate.
Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml

Other Notes

Small volumes of anti-BAG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BAG1 purchase

MBS221459 is an antibody targeting BAG1. Also known as RABBIT ANTI BAG1 (C-TERMINAL). Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BAG1. It may not necessarily be applicable to this product.

NCBI GI #

288915527

NCBI GeneID

573

NCBI Accession #

NP_001165886.1

UniProt Primary Accession #

Q99933

UniProt Secondary Accession #

O75315; Q14414; Q53H32; Q5VZE8; Q5VZE9; Q5VZF0; Q96TG2; Q9Y2V4

UniProt Related Accession #

Q99933

GenBank Nucleotide #

NM_001172415.1

NCBI Official Full Name

BAG family molecular chaperone regulator 1 isoform BAG-1S

NCBI Official Synonym Full Names

BCL2-associated athanogene

NCBI Official Symbol

BAG1

NCBI Official Synonym Symbols

HAP; BAG-1; RAP46

UniProt Protein Name

BAG family molecular chaperone regulator 1

UniProt Synonym Protein Names

Bcl-2-associated athanogene 1

Protein Family

BAG family molecular chaperone regulator

UniProt Gene Name

BAG1

UniProt Synonym Gene Names

HAP; BAG-1

UniProt Entry Name

BAG1_HUMAN

NCBI Protein Information

BAG family molecular chaperone regulator 1; Bcl-2 associating athanogene-1 protein; Bcl-2-binding protein; glucocortoid receptor-associated protein RAP46; receptor-associated protein, 46-KD

NCBI Summary for BAG1

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]

UniProt Comments for BAG1

BAG1: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2. Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium. Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9p12

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; chaperone binding; receptor signaling protein activity

Biological Process: cell surface receptor linked signal transduction; apoptosis; negative regulation of apoptosis

Research Articles on BAG1

1. distinct isoforms of BAG-1 had different anti-apoptotic ability in breast cancer cells treated with the 4-OH TAM.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-BAG1 antibody

Products by Pathway	Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram

Diseases associated with anti-BAG1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>16 publications with BAG1 and Nervous System Diseases
Disease Models, Animal Antibodies	>10 publications with BAG1 and Disease Models, Animal
Breast Neoplasms Antibodies	>9 publications with BAG1 and Breast Neoplasms
Cell Transformation, Neoplastic Antibodies	>4 publications with BAG1 and Cell Transformation, Neoplastic
Adenocarcinoma Antibodies	>4 publications with BAG1 and Adenocarcinoma
Nerve Degeneration Antibodies	>3 publications with BAG1 and Nerve Degeneration
Prostatic Neoplasms Antibodies	>3 publications with BAG1 and Prostatic Neoplasms
Neoplasms, Experimental Antibodies	>2 publications with BAG1 and Neoplasms, Experimental
Heart Diseases Antibodies	>2 publications with BAG1 and Heart Diseases
Inflammation Antibodies	>2 publications with BAG1 and Inflammation

Organs/Tissues associated with anti-BAG1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>126 publications with BAG1 and Muscle
Nerve Antibodies	>49 publications with BAG1 and Nerve
Brain Antibodies	>43 publications with BAG1 and Brain
Blood Antibodies	>25 publications with BAG1 and Blood
Embryonic Tissue Antibodies	>14 publications with BAG1 and Embryonic Tissue
Heart Antibodies	>9 publications with BAG1 and Heart
Eye Antibodies	>8 publications with BAG1 and Eye
Lung Antibodies	>6 publications with BAG1 and Lung
Liver Antibodies	>5 publications with BAG1 and Liver
Prostate Antibodies	>4 publications with BAG1 and Prostate