Rabbit Anti-TIM-4 Polyclonal Antibody – Cat# MBS221476

Western blot analysis of whole cell lysate from Jurkat T-cell leukemia cells probed with Rabbit anti Human TIM-4 (MBS221476) at 1(A) and 2(B) ug/ml

TIM-4 Antibody – Rabbit Polyclonal

Name: TIM-4 Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS221476
Price: 550 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221476

MBS221476 detects an epitope within the C-terminal (CT) region of T-cell immunoglobulin mucin-4 (TIM-4), also known as SMUCKLER. TIM-4 is a 42kD type I membrane protein which is preferentially expressed on macrophages and dendritic cells. TIM-4 is the ligand… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TIM-4, Polyclonal Antibody

Full Product Name

RABBIT ANTI TIM-4 (C-TERMINAL)

Product Gene Name

anti-TIM-4 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse, Rat

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 1.0mg/ml (lot specific)

Specificity

This item detects an epitope within the C-terminal (CT) region of T-cell immunoglobulin mucin-4 (TIM-4), also known as SMUCKLER. TIM-4 is a 42kD type I membrane protein which is preferentially expressed on macrophages and dendritic cells. TIM-4 is the ligand of TIM-1, and binding leads to T-cell proliferation and cytokine production. Polymorphisms in the TIM-4 gene may be associated with a predisposition to asthma.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

38,536 Da

OMIM

610096

3D Structure

ModBase 3D Structure for Q96H15

Perservative Stabilisers

0.02% Sodium Azide

Immunogen

15 amino acid peptide sequence near the carboxy terminus of human TIM-4.
Buffer Solution||Phosphate buffered saline

Antiserum Preparation

Antisera to human TIM-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-TIM-4 antibody

Western Blot (WB)

Application Notes for anti-TIM-4 antibody

Western Blot: This item detects a band of approximately 54kDa in Jurkat cell lysate.
Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 2.0ug/ml

Other Notes

Small volumes of anti-TIM-4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIM-4 purchase

MBS221476 is an antibody targeting TIM-4. Also known as RABBIT ANTI TIM-4 (C-TERMINAL). Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TIM-4. It may not necessarily be applicable to this product.

NCBI GI #

226498466

NCBI GeneID

91937

NCBI Accession #

NP_001140198.1

UniProt Primary Accession #

Q96H15

UniProt Secondary Accession #

B5MCL9

UniProt Related Accession #

Q96H15

GenBank Nucleotide #

NM_001146726.1

NCBI Official Full Name

T-cell immunoglobulin and mucin domain-containing protein 4 isoform 2

NCBI Official Synonym Full Names

T-cell immunoglobulin and mucin domain containing 4

NCBI Official Symbol

TIMD4

NCBI Official Synonym Symbols

TIM4; SMUCKLER

UniProt Protein Name

T-cell immunoglobulin and mucin domain-containing protein 4

UniProt Synonym Protein Names

T-cell immunoglobulin mucin receptor 4; TIM-4; T-cell membrane protein 4

Protein Family

T-cell immunoglobulin and mucin domain-containing protein

UniProt Gene Name

TIMD4

UniProt Synonym Gene Names

TIM4; TIMD-4; TIM-4

UniProt Entry Name

TIMD4_HUMAN

NCBI Protein Information

T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIM-4; TIMD-4

UniProt Comments for TIM-4

TIM-4: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane

Research Articles on TIM-4

1. Our study uncovers a novel ability of TIM-family proteins to block the release of HIV-1 and other viruses by interaction with virion- and cell-associated phosphatidylserine.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TIM-4 antibody

Disease Name	Pubmed Publications
Respiratory Hypersensitivity Antibodies	>7 publications with TIM-4 and Respiratory Hypersensitivity
Lung Diseases Antibodies	>6 publications with TIM-4 and Lung Diseases
Inflammation Antibodies	>4 publications with TIM-4 and Inflammation
Necrosis Antibodies	>3 publications with TIM-4 and Necrosis
Fetal Death Antibodies	>1 publications with TIM-4 and Fetal Death

Organs/Tissues associated with anti-TIM-4 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>4 publications with TIM-4 and Liver
Spleen Antibodies	>2 publications with TIM-4 and Spleen
Kidney Antibodies	>2 publications with TIM-4 and Kidney
Lymph Node Antibodies	>1 publications with TIM-4 and Lymph Node