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xCT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221510

Rabbit anti human xCT (N-terminal) antibody detects an epitope within the N-terminal (NT) of the cysteine/glutamate transporter xCT, which forms a complex with the 4F2 heavy chain (CD98), via a disulfide bond. This heterodimer forms a Na+ independent amino… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
xCT, Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN xCT (N-TERMINAL)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
55,423 Da
OMIM
607933
3D Structure
ModBase 3D Structure for Q9UPY5
Perservative Stabilisers
0.1% Sodium Azide (NaN3)
Histology Positive Control Tissue
Neurons or astrocytes
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to human xCT were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-xCT antibody
Immunohistology Paraffin*
Application Notes for anti-xCT antibody
Immunohistology - Paraffin: Minimum Dilution: 20; Maximum Dilution: 30ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Testing Data #1 of anti-xCT antibody

Staining of formalin fixed, paraffin embedded human chief wells with Rabbit anti Human xCT (MBS221510)
Rabbit Anti-xCT Polyclonal Antibody – Cat# MBS221510

Other Notes

Small volumes of anti-xCT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for xCT purchase

MBS221510 is an antibody targeting xCT. Also known as RABBIT ANTI HUMAN xCT (N-TERMINAL). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for xCT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cystine/glutamate transporter
NCBI Official Synonym Full Names
solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
NCBI Official Symbol
SLC7A11
NCBI Official Synonym Symbols
xCT; CCBR1
UniProt Protein Name
Cystine/glutamate transporter
UniProt Synonym Protein Names
Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1; Solute carrier family 7 member 11; xCT
UniProt Gene Name
SLC7A11
UniProt Entry Name
XCT_HUMAN
NCBI Protein Information
cystine/glutamate transporter; amino acid transport system xc-; calcium channel blocker resistance protein CCBR1; solute carrier family 7 member 11; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11

NCBI Summary for xCT

This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death. [provided by RefSeq, Sep 2011]
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UniProt Comments for xCT

SLC7A11: Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q28.3

Cellular Component: cell surface; cytoskeleton; rough endoplasmic reticulum; plasma membrane; integral to membrane

Molecular Function: protein binding; cystine:glutamate antiporter activity

Biological Process: response to nicotine; amino acid transport; response to toxin; ion transport; brain development; response to oxidative stress; blood coagulation; transmembrane transport; leukocyte migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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