Rabbit Anti-MUNC-18 Polyclonal Antibody – Cat# MBS221521

MUNC-18 Antibody – Rabbit Polyclonal

Name: MUNC-18 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS221521
Price: 740 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221521

MBS221521 recognises Munc-18 phosphorylated at serine 515, a 67 kDa member of the STXBP/UNC-18/SEC1 family. It is neural specific, expressed in the brain, spinal cord and at very high levels in axons. Munc-18 is crucial to calcium-dependent neurotransmitter… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MUNC-18, Polyclonal Antibody

Full Product Name

RABBIT ANTI MUNC-18 (pSer515)

Product Gene Name

anti-MUNC-18 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence), Xenopus (Expected from Sequence)

Form/Format

Purified
Purified IgG - liquid

Specificity

This item recognises Munc-18 phosphorylated at serine 515, a 67 kDa member of the STXBP/UNC-18/SEC1 family. It is neural specific, expressed in the brain, spinal cord and at very high levels in axons. Munc-18 is crucial to calcium-dependent neurotransmitter release, facilitating synaptic vesicle docking and fusion. It inhibits the formation of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex by binding syntaxin 1 preventing the other components (synaptobrevin and synaptosomal associated protein of 25 kDa (SNAP-25)) from binding. The function of Munc-18 is regulated by the phosphorylation of serine 515 by protein kinase C.

Munc-18 is also involved in the onset of female puberty through its regulation of glutamate release.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.

Molecular Weight

68,736 Da

3D Structure

ModBase 3D Structure for P61765

Perservative Stabilisers

0.09% Sodium Azide (NaN3)
50% Glycerol
0.01% Bovine Serum Albumin

Immunogen

Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated Ser515 of Munc-18.
Buffer Solution||10mM Hepes pH7.5

Antiserum Preparation

Antisera to rat Munc-18 (pSer515) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.

Target Species

Rat

Applications Tested/Suitable for anti-MUNC-18 antibody

Western Blot (WB)*

Application Notes for anti-MUNC-18 antibody

Western Blot: This item detects a band/s of approximately 65kDa in rat cortex cell lysates
Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Other Notes

Small volumes of anti-MUNC-18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MUNC-18 purchase

MBS221521 is an antibody targeting MUNC-18. Also known as RABBIT ANTI MUNC-18 (pSer515). Reactive in Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence), Xenopus (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MUNC-18. It may not necessarily be applicable to this product.

NCBI GI #

6981602

NCBI GeneID

25558

NCBI Accession #

NP_037170.1

UniProt Primary Accession #

P61765

UniProt Secondary Accession #

Q28208; Q62759; Q64320; Q96TG8

UniProt Related Accession #

P61765

GenBank Nucleotide #

NM_013038.3

NCBI Official Full Name

syntaxin-binding protein 1

NCBI Official Synonym Full Names

syntaxin binding protein 1

NCBI Official Symbol

Stxbp1

NCBI Official Synonym Symbols

Sec1; nSec1; NSEC1A; n-sec1; rbSec1; ANC18HA; Unc18-1; rbSec1A; rbSec1B; Munc18-1

UniProt Protein Name

Syntaxin-binding protein 1

UniProt Synonym Protein Names

N-Sec1; Protein unc-18 homolog 1; Unc18-1; Protein unc-18 homolog A; Unc-18A; p67; rbSec1

Protein Family

Syntaxin-binding protein

UniProt Gene Name

Stxbp1

UniProt Synonym Gene Names

Unc18a; Unc18-1; Unc-18A

UniProt Entry Name

STXB1_RAT

NCBI Protein Information

syntaxin-binding protein 1; p67; protein unc-18 homolog 1; protein unc-18 homolog A; unc-18-1; unc-18A

NCBI Summary for MUNC-18

inhibits binding of synaptobrevin and SNAP-25 to syntaxin 1A to modulate SNARE complex formation; acts to regulate neurotransmission [RGD, Feb 2006]

UniProt Comments for MUNC-18

STXBP1: may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Two splice-variant isoforms have been described.

Protein type: Vesicle

Cellular Component: nucleoplasm; protein complex; mitochondrion; cell; cytoplasm; plasma membrane; terminal button; cytosol; myelin sheath

Molecular Function: protein domain specific binding; identical protein binding; protein binding; SNARE binding; syntaxin binding; syntaxin-1 binding; protein N-terminus binding; protein kinase binding

Biological Process: exocytosis; protein stabilization; synaptic vesicle maturation; neurotransmitter secretion; positive regulation of calcium ion-dependent exocytosis; protein transport; platelet degranulation; axon target recognition; neuromuscular synaptic transmission; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; vesicle docking during exocytosis; positive regulation of exocytosis

Research Articles on MUNC-18

1. SEC1/Munc18-SNARE interactions at other transport steps may provide a general mechanism to drive lipid bilayer merger.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MUNC-18 antibody

Products by Pathway	Pathway Diagram
Acetylcholine Neurotransmitter Release Cycle Pathway antibodies	Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies	GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
Glutamate Neurotransmitter Release Cycle Pathway antibodies	Glutamate Neurotransmitter Release Cycle Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Integration Of Energy Metabolism Pathway antibodies	Integration Of Energy Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Neurotransmitter Release Cycle Pathway antibodies	Neurotransmitter Release Cycle Pathway Diagram
Norepinephrine Neurotransmitter Release Cycle Pathway antibodies	Norepinephrine Neurotransmitter Release Cycle Pathway Diagram

Diseases associated with anti-MUNC-18 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>49 publications with MUNC-18 and Nervous System Diseases
Brain Diseases Antibodies	>44 publications with MUNC-18 and Brain Diseases
Neurobehavioral Manifestations Antibodies	>11 publications with MUNC-18 and Neurobehavioral Manifestations
Epilepsies, Myoclonic Antibodies	>7 publications with MUNC-18 and Epilepsies, Myoclonic
Inflammation Antibodies	>2 publications with MUNC-18 and Inflammation
Cognition Disorders Antibodies	>2 publications with MUNC-18 and Cognition Disorders
Memory Disorders Antibodies	>2 publications with MUNC-18 and Memory Disorders
Disease Models, Animal Antibodies	>2 publications with MUNC-18 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>1 publications with MUNC-18 and Cardiovascular Diseases
Heart Diseases Antibodies	>1 publications with MUNC-18 and Heart Diseases

Organs/Tissues associated with anti-MUNC-18 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>70 publications with MUNC-18 and Brain
Embryonic Tissue Antibodies	>10 publications with MUNC-18 and Embryonic Tissue
Pancreas Antibodies	>8 publications with MUNC-18 and Pancreas
Heart Antibodies	>4 publications with MUNC-18 and Heart
Eye Antibodies	>4 publications with MUNC-18 and Eye
Testis Antibodies	>3 publications with MUNC-18 and Testis
Ovary Antibodies	>2 publications with MUNC-18 and Ovary
Pineal Gland Antibodies	>2 publications with MUNC-18 and Pineal Gland
Adrenal Gland Antibodies	>2 publications with MUNC-18 and Adrenal Gland
Dorsal Root Ganglion Antibodies	>2 publications with MUNC-18 and Dorsal Root Ganglion