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GDNFR ALPHA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221584

Rabbit anti GDNFalpha antibody recognizes GDNFR alpha, also known as GFR&alpha-1 (GFRa Nomenclature Committee 1997), GDNF receptor alpha-1, RET ligand 1 or TGF-beta-related neurotrophic factor receptor 1. GFR&alpha-1 is a 465 amino acid ~52 kDa GPI anchored… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
GDNFR ALPHA, Polyclonal Antibody
Full Product Name
RABBIT ANTI GDNFR ALPHA
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (lot specific)
Specificity
This item recognises GDNFR alpha, a cell surface receptor for glial cell derived neutrophic factor (GDNF). GDNFR alpha has more recently been designated GFR alpha -1.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
50,838 Da
OMIM
601496
3D Structure
ModBase 3D Structure for P56159
Perservative Stabilisers
0.02% Sodium Azide
Immunogen
Peptide from C terminus of human GDNFR alpha
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to human GDNFR alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Applications Tested/Suitable for anti-GDNFR ALPHA antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-GDNFR ALPHA antibody
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1000

Testing Data #1 of anti-GDNFR ALPHA antibody

Western blot analysis of crude membrane fraction lysates from human brain (B), liver (L), kidney (K) and spleen(S) probed with Rabbit anti Human GDNFR alpha (MBS221584)
Rabbit Anti-GDNFR ALPHA Polyclonal Antibody – Cat# MBS221584

Testing Data #2 of anti-GDNFR ALPHA antibody

Immunohistochemical staining of of human brain with Rabbit anti Human GDNFR alpha (MBS221584)
Rabbit Anti-GDNFR ALPHA Polyclonal Antibody, Testing Data #2 – Cat# MBS221584

Other Notes

Small volumes of anti-GDNFR ALPHA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GDNFR ALPHA purchase

MBS221584 is an antibody targeting GDNFR ALPHA. Also known as RABBIT ANTI GDNFR ALPHA. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GDNFR ALPHA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
GDNF family receptor alpha-1 isoform b preproprotein
NCBI Official Synonym Full Names
GDNF family receptor alpha 1
NCBI Official Symbol
GFRA1
NCBI Official Synonym Symbols
GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1
UniProt Protein Name
GDNF family receptor alpha-1
UniProt Synonym Protein Names
RET ligand 1; TGF-beta-related neurotrophic factor receptor 1
Protein Family
UniProt Gene Name
GFRA1
UniProt Synonym Gene Names
GDNFRA; RETL1; TRNR1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1
UniProt Entry Name
GFRA1_HUMAN
NCBI Protein Information
GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GPI-linked anchor protein; Glial cell line-derived neurotrophic factor receptor alpha; PI-linked cell-surface accessory protein; RET ligand 1; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1

NCBI Summary for GDNFR ALPHA

Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Feb 2009]
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UniProt Comments for GDNFR ALPHA

GFRA1: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Receptor, misc.

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: extrinsic to membrane; plasma membrane

Molecular Function: glial cell line-derived neurotrophic factor receptor activity; receptor binding

Biological Process: axon guidance; cell surface receptor linked signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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