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Aquaporin 1 (AQP-1) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS029349

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of AQP-1 (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates,… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Aquaporin 1 (AQP-1), ELISA Kit
Full Product Name
Rat Aquaporin 1 ELISA Kit
Product Gene Name
AQP-1 elisa kit
Species Reactivity
Rat
Assay Type
Sandwich
Detection Range
0.25 ng/ml - 8 ng/ml.
Sensitivity
0.1 ng/ml.
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
28,793 Da
Chromosome Location
Chromosome: 6; NC_000072.6 (55336299..55348555). Location: 6 B3; 6 27.38 cM
3D Structure
ModBase 3D Structure for Q02013

Other Notes

Small volumes of AQP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AQP-1 purchase

MBS029349 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aquaporin 1 (AQP-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AQP-1. The ELISA analytical biochemical technique of the MBS029349 kit is based on AQP-1 antibody-AQP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AQP-1. It may not necessarily be applicable to this product.

NCBI GI #
6680710
NCBI GeneID
11826
NCBI Accession #
NP_031498.1
UniProt Primary Accession #
Q02013
UniProt Secondary Accession #
Q542P1; Q91VY8
UniProt Related Accession #
Q02013
GenBank Nucleotide #
NM_007472.2
NCBI Official Full Name
aquaporin-1
NCBI Official Synonym Full Names
aquaporin 1
NCBI Official Symbol
Aqp1
NCBI Official Synonym Symbols
CHIP28
UniProt Protein Name
Aquaporin-1
UniProt Synonym Protein Names
Aquaporin-CHIP; Delayed early response protein 2; DER2; Water channel protein for red blood cells and kidney proximal tubule
Protein Family
Aquaporin
UniProt Gene Name
Aqp1
UniProt Synonym Gene Names
AQP-1; DER2
UniProt Entry Name
AQP1_MOUSE
NCBI Protein Information
aquaporin-1; DER2; AQP-1; aquaporin-CHIP; delayed early response protein 2; growth factor-induced delayed early response protein; water channel protein for red blood cells and kidney proximal tubule

UniProt Comments for AQP-1

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, aquaporin family; Membrane protein, integral

Cellular Component: neuron projection; nuclear membrane; brush border membrane; basolateral plasma membrane; integral to plasma membrane; cell; integral to membrane; dense core granule membrane; membrane; apical part of cell; axon; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: potassium channel activity; ephrin receptor binding; water transporter activity; transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; glycerol transmembrane transporter activity; potassium ion transmembrane transporter activity; water channel activity; nitric oxide transporter activity; transmembrane transporter activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; wound healing; carbon dioxide transport; cellular response to stress; water transport; negative regulation of caspase activity; sensory perception of pain; positive regulation of saliva secretion; water homeostasis; positive regulation of fibroblast proliferation; organic cation transport; nitric oxide transport; transport; cellular homeostasis; transmembrane transport; potassium ion transport; lipid digestion; secretion by cell; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; establishment and/or maintenance of actin cytoskeleton polarity; cell volume homeostasis; negative regulation of apoptosis; positive regulation of cell migration
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Research Articles on AQP-1

1. Cardioplegia, ischemia and hypoxia decrease AQP1 mRNA as well as total protein expression and glycosylation, possibly regulated by miR-214.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with AQP-1 elisa kit

 Products by Pathway  Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 O2/CO2 Exchange In Erythrocytes Pathway antibodies  O2/CO2 Exchange In Erythrocytes Pathway Diagram
 Passive Transport By Aquaporins Pathway antibodies  Passive Transport By Aquaporins Pathway Diagram
 Proximal Tubule Bicarbonate Reclamation Pathway antibodies  Proximal Tubule Bicarbonate Reclamation Pathway Diagram
 Proximal Tubule Bicarbonate Reclamation Pathway antibodies  Proximal Tubule Bicarbonate Reclamation Pathway Diagram
 Regulation Of Water Balance By Renal Aquaporins Pathway antibodies  Regulation Of Water Balance By Renal Aquaporins Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with AQP-1 elisa kit

 Disease Name  Pubmed Publications
 Edema Antibodies  >138 publications with AQP-1 and Edema
 Kidney Diseases Antibodies  >114 publications with AQP-1 and Kidney Diseases
 Neoplasms Antibodies  >94 publications with AQP-1 and Neoplasms
 Nervous System Diseases Antibodies  >85 publications with AQP-1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >81 publications with AQP-1 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >57 publications with AQP-1 and Cardiovascular Diseases
 Lung Diseases Antibodies  >45 publications with AQP-1 and Lung Diseases
 Carcinoma Antibodies  >43 publications with AQP-1 and Carcinoma
 Fibrosis Antibodies  >30 publications with AQP-1 and Fibrosis
 Inflammation Antibodies  >27 publications with AQP-1 and Inflammation

Organs/Tissues associated with AQP-1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >373 publications with AQP-1 and Kidney
 Brain Antibodies  >184 publications with AQP-1 and Brain
 Lung Antibodies  >130 publications with AQP-1 and Lung
 Eye Antibodies  >129 publications with AQP-1 and Eye
 Muscle Antibodies  >56 publications with AQP-1 and Muscle
 Salivary Gland Antibodies  >55 publications with AQP-1 and Salivary Gland
 Liver Antibodies  >52 publications with AQP-1 and Liver
 Heart Antibodies  >50 publications with AQP-1 and Heart
 Intestine Antibodies  >38 publications with AQP-1 and Intestine
 Artery Antibodies  >35 publications with AQP-1 and Artery
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