Goat Anti-NCF1 Polyclonal Antibody – Cat# MBS221668

Western blot analysis of U937 cell lysate probed with Goat anti Human NCF1 (MBS221668)

NCF1 Antibody – Goat Polyclonal

Name: NCF1 Antibody; Polyclonal; Goat; MyBioSource
Brand: MyBioSource
SKU: MBS221668
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221668

MBS221668 recognises neutrophil cytosolic factor 1 (NCF1), a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

NCF1, Polyclonal Antibody

Full Product Name

GOAT ANTI HUMAN NCF1

Product Gene Name

anti-NCF1 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Bovine (Expected from Sequence), Dolphin (Expected from Sequence), Mouse (Expected from Sequence), Rabbit (Expected from Sequence), Rat (Expected from Sequence)

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 0.5 mg/ml (lot specific)

Specificity

This item recognises neutrophil cytosolic factor 1 (NCF1), a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

30,781 Da

OMIM

233700

3D Structure

ModBase 3D Structure for P14598

Perservative Stabilisers

0.02% Sodium Azide
0.5% Bovine Serum Albumin

Immunogen

Peptide sequence CSESTKRKLASAV corresponding to the C-terminus of NCF1 (NP_000256).
Buffer Solution||TRIS buffered saline

Antiserum Preparation

Antisera to NCF1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-NCF1 antibody

Western Blot (WB)

Application Notes for anti-NCF1 antibody

Western Blot: This item detects a band of approximately 47kDa in U937 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
Western Blotting: Minimum Dilution: 0.2ug/ml; Maximum Dilution: 2ug/ml

Other Notes

Small volumes of anti-NCF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NCF1 purchase

MBS221668 is an antibody targeting NCF1. Also known as GOAT ANTI HUMAN NCF1. Reactive in Bovine (Expected from Sequence), Dolphin (Expected from Sequence), Mouse (Expected from Sequence), Rabbit (Expected from Sequence), Rat (Expected from Sequence). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for NCF1. It may not necessarily be applicable to this product.

NCBI GI #

528078313

NCBI GeneID

653361

NCBI Accession #

NP_000256.4

UniProt Primary Accession #

P14598

UniProt Secondary Accession #

O43842; Q2PP07; Q53FR5; Q9BU90; Q9BXI7; Q9BXI8; Q9UDV9; Q9UMU2; A6NEH2; A8K7S9

UniProt Related Accession #

P14598

GenBank Nucleotide #

NM_000265.5

NCBI Official Full Name

neutrophil cytosol factor 1

NCBI Official Synonym Full Names

neutrophil cytosolic factor 1

NCBI Official Symbol

NCF1

NCBI Official Synonym Symbols

NCF1A; NOXO2; p47phox; SH3PXD1A

UniProt Protein Name

Neutrophil cytosol factor 1

UniProt Synonym Protein Names

47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox

Protein Family

Neutrophil cytosol factor

UniProt Gene Name

NCF1

UniProt Synonym Gene Names

NOXO2; SH3PXD1A; NCF-1

UniProt Entry Name

NCF1_HUMAN

NCBI Protein Information

neutrophil cytosol factor 1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF-1; NCF-47K; SH3 and PX domain-containing protein 1A; neutrophil NADPH oxidase factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); nox organizer 2; nox-organizing protein 2; p47-phox

NCBI Summary for NCF1

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

UniProt Comments for NCF1

p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Protein type: Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: Golgi apparatus; extrinsic to membrane; rough endoplasmic reticulum; cell soma; dendrite; cytosol; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; superoxide-generating NADPH oxidase activity; phosphatidylinositol-3,4-bisphosphate binding; SH3 domain binding; phosphoinositide binding

Biological Process: respiratory burst during defense response; respiratory burst; interaction with host; apoptosis; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; leukotriene metabolic process; cell proliferation; antigen processing and presentation of peptide antigen via MHC class I; negative regulation of smooth muscle contraction; antigen processing and presentation of exogenous peptide antigen via MHC class I; cellular defense response; innate immune response; response to yeast; vascular endothelial growth factor receptor signaling pathway; protein targeting to membrane; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I

Research Articles on NCF1

1. Four novel mutations in the NCF1, NCF2, and CYBB genees have been identified in chronic granulomatous disease patients in Morocco.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NCF1 antibody

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Processing-Cross Presentation Pathway antibodies	Antigen Processing-Cross Presentation Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway antibodies	Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram

Diseases associated with anti-NCF1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>47 publications with NCF1 and Inflammation
Disease Models, Animal Antibodies	>40 publications with NCF1 and Disease Models, Animal
Arthritis, Rheumatoid Antibodies	>24 publications with NCF1 and Arthritis, Rheumatoid
Cardiovascular Diseases Antibodies	>11 publications with NCF1 and Cardiovascular Diseases
Heart Diseases Antibodies	>8 publications with NCF1 and Heart Diseases
Necrosis Antibodies	>7 publications with NCF1 and Necrosis
Hypertension Antibodies	>5 publications with NCF1 and Hypertension
Liver Diseases Antibodies	>3 publications with NCF1 and Liver Diseases
Diabetes Mellitus, Experimental Antibodies	>3 publications with NCF1 and Diabetes Mellitus, Experimental
Atherosclerosis Antibodies	>2 publications with NCF1 and Atherosclerosis

Organs/Tissues associated with anti-NCF1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>51 publications with NCF1 and Blood
Lung Antibodies	>10 publications with NCF1 and Lung
Lymph Antibodies	>7 publications with NCF1 and Lymph
Lymph Node Antibodies	>7 publications with NCF1 and Lymph Node
Spleen Antibodies	>6 publications with NCF1 and Spleen
Brain Antibodies	>4 publications with NCF1 and Brain
Skin Antibodies	>4 publications with NCF1 and Skin
Kidney Antibodies	>3 publications with NCF1 and Kidney
Connective Tissue Antibodies	>2 publications with NCF1 and Connective Tissue
Placenta Antibodies	>1 publications with NCF1 and Placenta