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BINDING Component 3 (BCL-2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221697

MBS221697 recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3. Bcl-2 binding component 3 is a pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localised to the mitochondria, binds BCL-2… More details

$670 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BCL-2 BINDING COMPONENT 3, Polyclonal Antibody
Full Product Name
RABBIT ANTI BCL-2 BINDING COMPONENT 3
Product Gene Name
anti-BCL-2 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse (Expected from Sequence)
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Specificity
This item recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3. Bcl-2 binding component 3 is a pro-apoptotic member of the BCL-2 family.

Bcl-2 binding component 3 is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
10,326 Da
OMIM
605854
3D Structure
ModBase 3D Structure for Q9BXH1
Perservative Stabilisers
0.02% Sodium Azide
Immunogen
Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3.
Histology Positive Control Tissue||Human breast carcinoma
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to human Bcl-2 binding component 3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-BCL-2 antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-BCL-2 antibody
Western Blot: This item detects a band of approximately 23kDa in K562 cell lysates.
Immunohistology - Paraffin: Maximum Dilution: 10 ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 2ug/ml; Maximum Dilution: 4ug/ml

Testing Data #1 of anti-BCL-2 antibody

Staining of human breast carcinoma. Formalin fixed paraffin processed tissue with Rabbit anti Human PUMA (NT) at 10 ug/ml (MBS221697)
Rabbit Anti-BCL-2 Polyclonal Antibody – Cat# MBS221697

Testing Data #2 of anti-BCL-2 antibody

Western blot analysis of whole cell lysate from K562 cells probed with Rabbit anti Human bcl-2 binding component 3 (MBS221697)
Rabbit Anti-BCL-2 Polyclonal Antibody, Testing Data #2 – Cat# MBS221697

Other Notes

Small volumes of anti-BCL-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BCL-2 purchase

MBS221697 is an antibody targeting BCL-2. Also known as RABBIT ANTI BCL-2 BINDING COMPONENT 3. Reactive in Mouse (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BCL-2. It may not necessarily be applicable to this product.

NCBI GI #
187829730
NCBI GeneID
27113
NCBI Accession #
NP_001120712.1
UniProt Primary Accession #
Q9BXH1
UniProt Secondary Accession #
O00171; Q96PG9; B9EGI3
UniProt Related Accession #
Q96PG8; Q9BXH1
GenBank Nucleotide #
NM_001127240.2
NCBI Official Full Name
bcl-2-binding component 3 isoform 1
NCBI Official Synonym Full Names
BCL2 binding component 3
NCBI Official Symbol
BBC3
NCBI Official Synonym Symbols
JFY1; PUMA; JFY-1
UniProt Protein Name
Bcl-2-binding component 3
UniProt Synonym Protein Names
JFY-1; p53 up-regulated modulator of apoptosis
Protein Family
Bcl-2-binding component
UniProt Gene Name
BBC3
UniProt Synonym Gene Names
PUMA
UniProt Entry Name
BBC3_HUMAN
NCBI Protein Information
bcl-2-binding component 3; p53 up-regulated modulator of apoptosis

NCBI Summary for BCL-2

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
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UniProt Comments for BCL-2

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; apoptosis; positive regulation of protein homooligomerization; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; reduction of endoplasmic reticulum calcium ion concentration; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
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Research Articles on BCL-2

1. PUMA depletion is sufficient to prevent the sensitization to apoptosis that occurs when autophagy is blocked.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BCL-2 antibody

 Reference Product  PubMed Publications
 PMAIP1 antibody  >73 publications with BCL-2 and PMAIP1
 TP53 antibody  >59 publications with BCL-2 and TP53
 BCL2L1 antibody  >46 publications with BCL-2 and BCL2L1
 BID antibody  >26 publications with BCL-2 and BID
 BAD antibody  >24 publications with BCL-2 and BAD
 MCL1 antibody  >17 publications with BCL-2 and MCL1
 SP1 antibody  >3 publications with BCL-2 and SP1
 SNAI2 antibody  >3 publications with BCL-2 and SNAI2
 BCL2L2 antibody  >3 publications with BCL-2 and BCL2L2

Pathways associated with anti-BCL-2 antibody

 Products by Pathway  Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of PUMA And Translocation To Mitochondria Pathway antibodies  Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway antibodies  BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram

Diseases associated with anti-BCL-2 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >74 publications with BCL-2 and Carcinoma
 Adenocarcinoma Antibodies  >34 publications with BCL-2 and Adenocarcinoma
 Breast Neoplasms Antibodies  >32 publications with BCL-2 and Breast Neoplasms
 Lung Neoplasms Antibodies  >24 publications with BCL-2 and Lung Neoplasms
 Necrosis Antibodies  >20 publications with BCL-2 and Necrosis
 Liver Diseases Antibodies  >19 publications with BCL-2 and Liver Diseases
 Lung Diseases Antibodies  >17 publications with BCL-2 and Lung Diseases
 Nervous System Diseases Antibodies  >16 publications with BCL-2 and Nervous System Diseases
 Inflammation Antibodies  >13 publications with BCL-2 and Inflammation
 Carcinoma, Squamous Cell Antibodies  >13 publications with BCL-2 and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-BCL-2 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >30 publications with BCL-2 and Lung
 Brain Antibodies  >18 publications with BCL-2 and Brain
 Kidney Antibodies  >13 publications with BCL-2 and Kidney
 Skin Antibodies  >12 publications with BCL-2 and Skin
 Prostate Antibodies  >12 publications with BCL-2 and Prostate
 Stomach Antibodies  >12 publications with BCL-2 and Stomach
 Muscle Antibodies  >9 publications with BCL-2 and Muscle
 Bone Antibodies  >9 publications with BCL-2 and Bone
 Eye Antibodies  >7 publications with BCL-2 and Eye
 Intestine Antibodies  >6 publications with BCL-2 and Intestine
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