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MCL-1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221875

MBS221875 is an antibody targeting Mcl-1. Also known as RABBIT ANTI HUMAN Mcl-1. Host: Rabbit. More details

$635 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mcl-1, Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN Mcl-1
Product Gene Name
anti-Mcl-1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
This item recognises an epitope within the central region (IN) of human Myeloid cell leukaemia sequence 1 (MCL-1), an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumours.

The MCL-1 protein, localised to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival.

This item recognises both the MCL-1L (38kDa) and MCL-1S (30kDa) isoforms of MCL-1.
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
OMIM
159552
3D Structure
ModBase 3D Structure for Q07820
Perservative Stabilisers
0.02% Sodium Azide
Immunogen
A 16 amino acid peptide located near the centre of human MCL-1.
Buffer Solution
Phosphate buffered saline
Antiserum Preparation
Antisera to human MCL-1 (IN) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Target Species
Human
Applications Tested/Suitable for anti-Mcl-1 antibody
Western Blot (WB)
Application Notes for anti-Mcl-1 antibody
Western Blot: This item detects a band of approximately 30kDa and 38kDa in Human Raji cell lysates.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Testing Data #1 of anti-Mcl-1 antibody

Western blot analysis of whole cell lysate from Raji, Burkitt's lymphoma derived B-lymphoblastoid cells probed with Rabbit anti Human Mcl-1 (MBS221875) at 0.5(A), 1(B) and 2(C) ug/ml
Rabbit Anti-MCL-1 Polyclonal Antibody – Cat# MBS221875

Testing Data #2 of anti-Mcl-1 antibody

Immunocytochemical staining of Raji cells with Rabbit anti Human Mcl-1 (MBS221875)
Rabbit Anti-MCL-1 Polyclonal Antibody, Testing Data #2 – Cat# MBS221875

Other Notes

Small volumes of anti-Mcl-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Mcl-1. It may not necessarily be applicable to this product.

NCBI GI #
309747067
NCBI GeneID
4170
NCBI Accession #
NP_001184249.1
UniProt Primary Accession #
Q07820
UniProt Secondary Accession #
Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04
UniProt Related Accession #
Q07820
GenBank Nucleotide #
NM_001197320.1
NCBI Official Full Name
induced myeloid leukemia cell differentiation protein Mcl-1 isoform 3
NCBI Official Synonym Full Names
myeloid cell leukemia 1
NCBI Official Symbol
MCL1
NCBI Official Synonym Symbols
TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
UniProt Protein Name
Induced myeloid leukemia cell differentiation protein Mcl-1
UniProt Synonym Protein Names
Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
Protein Family
Induced myeloid leukemia cell differentiation protein
UniProt Gene Name
MCL1
UniProt Synonym Gene Names
BCL2L3; Bcl2-L-3
UniProt Entry Name
MCL1_HUMAN
NCBI Protein Information
induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related)

NCBI Summary for Mcl-1

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]
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UniProt Comments for Mcl-1

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Channel, misc.; Membrane protein, integral; Apoptosis; Inhibitor; Mitochondrial

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cell fate determination; cellular homeostasis
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Research Articles on Mcl-1

1. Results position TWEAK-Fn14 signaling through Mcl-1 as a significant mechanism for NSCLC tumor cell survival.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Mcl-1 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 IL-7 Signaling Pathway antibodies  IL-7 Signaling Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram

Diseases associated with anti-Mcl-1 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >60 publications with Mcl-1 and Adenocarcinoma
 Lung Neoplasms Antibodies  >56 publications with Mcl-1 and Lung Neoplasms
 Necrosis Antibodies  >49 publications with Mcl-1 and Necrosis
 Breast Neoplasms Antibodies  >42 publications with Mcl-1 and Breast Neoplasms
 Liver Diseases Antibodies  >38 publications with Mcl-1 and Liver Diseases
 Liver Neoplasms Antibodies  >32 publications with Mcl-1 and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >32 publications with Mcl-1 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >30 publications with Mcl-1 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >28 publications with Mcl-1 and Inflammation
 Multiple Myeloma Antibodies  >28 publications with Mcl-1 and Multiple Myeloma

Organs/Tissues associated with anti-Mcl-1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >150 publications with Mcl-1 and Blood
 Lung Antibodies  >79 publications with Mcl-1 and Lung
 Liver Antibodies  >70 publications with Mcl-1 and Liver
 Bone Antibodies  >60 publications with Mcl-1 and Bone
 Bone Marrow Antibodies  >44 publications with Mcl-1 and Bone Marrow
 Brain Antibodies  >39 publications with Mcl-1 and Brain
 Prostate Antibodies  >30 publications with Mcl-1 and Prostate
 Vascular Antibodies  >22 publications with Mcl-1 and Vascular
 Skin Antibodies  >19 publications with Mcl-1 and Skin
 Lymph Node Antibodies  >15 publications with Mcl-1 and Lymph Node
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