Goat Anti-CALR Polyclonal Antibody – Cat# MBS222424

Calreticulin (CALR) Antibody – Goat Polyclonal

Name: CALR Antibody; Polyclonal; Goat; MyBioSource
Brand: MyBioSource
SKU: MBS222424
Price: 465 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS222424

Goat anti calreticulin antibodyrecognises calreticulin, a 418 aminoacid ~60 kDa Ca2+ binding chaperone involved in protein modification and folding within the endoplasmic reticulum (UniProt: P15253). More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CALRETICULIN, Polyclonal Antibody

Full Product Name

GOAT ANTI CALRETICULIN

Product Gene Name

anti-CALR antibody

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Human, Mouse, Rabbit, Rat

Form/Format

Serum
Serum - liquid

Specificity

This item recognises calreticulin, a 60kD protein that has vital Ca2+ binding functions and is also involved in protein modification and folding within the endoplasmic reticulum.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

48,275 Da

3D Structure

ModBase 3D Structure for P15253

Perservative Stabilisers

0.09% Sodium Azide

Immunogen

Purified rabbit skeletal muscle calreticulin
Buffer Solution||Glycine buffered saline

Antiserum Preparation

Antisera to calreticulin were raised by repeated immunisation of goats with highly purified antigen.

Applications Tested/Suitable for anti-CALR antibody

Immunohistology Frozen, Western Blot (WB)

Application Notes for anti-CALR antibody

Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/70;
Western Blotting: Minimum Dilution: 1/300; Maximum Dilution: 1/500

Product References and Citations for anti-CALR antibody

Calreticulin Regulates Neointima Formation and Collagen Deposition following Carotid Artery Ligation. Zimmerman K.A., Xing D., Pallero M.A., Lu A., Ikawa M., Black L., Hoyt K.L., Kabarowski J.H., Michalak M., Murphy-Ullrich J.E (2016)

Other Notes

Small volumes of anti-CALR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CALR purchase

Long considered the standard biomarker for endoplasmic reticulum, calreticulin - CALR - protein is frequently used in double labeling biol chem experiments, western blot of cell lysates, and cells or tissue immunocytochemistry. With the advent of a third species - goat - calreticulin antibody, triple label custom application are now possible. The wide range of species reactivity, human mouse rat rabbit, from goat serum antibody production of IgG and specific product reaction has made usage of this anti calreticulin antibody popular, judged by the number of PubMed citations and article (s). Immunizing antigen is of rabbit origin, so donkey or sheep anti goat IgG Alexa fluorâ® are preferred over rabbit IgG Alexa anti goat. Anti goat secondary antibodies are an advantage for signal to noise ratios over competitors goat anti rabbit products for calreticulin marker antibodies. Additionally, this custom antibody has been validated by immunohistochemistry and western blot for specificity.

Additional Details

NCBI/Uniprot data below describe general gene information for CALR. It may not necessarily be applicable to this product.

NCBI GI #

126723562

NCBI GeneID

100009050

NCBI Accession #

NP_001075704.1

UniProt Primary Accession #

P15253

UniProt Related Accession #

P15253

GenBank Nucleotide #

NM_001082235.1

NCBI Official Full Name

calreticulin

NCBI Official Symbol

CALR

UniProt Protein Name

Calreticulin

UniProt Synonym Protein Names

CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP

Protein Family

Calreticulin

UniProt Gene Name

CALR

UniProt Synonym Gene Names

ERp60

UniProt Entry Name

CALR_RABIT

NCBI Protein Information

calreticulin; CRP55; ERp60; HACBP; calregulin; endoplasmic reticulum resident protein 60

UniProt Comments for CALR

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis ().

Research Articles on CALR

1. These findings suggest that protein kinase C is involved in the regulation of CRT function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CALR antibody

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Phagosome Pathway antibodies	Phagosome Pathway Diagram
Phagosome Pathway antibodies	Phagosome Pathway Diagram
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Diseases associated with anti-CALR antibody

Disease Name	Pubmed Publications
Anemia Antibodies	>11 publications with CALR and Anemia
Fibrosis Antibodies	>5 publications with CALR and Fibrosis
Disease Models, Animal Antibodies	>4 publications with CALR and Disease Models, Animal
Liver Neoplasms Antibodies	>3 publications with CALR and Liver Neoplasms
Hepatomegaly Antibodies	>3 publications with CALR and Hepatomegaly
Cognition Disorders Antibodies	>3 publications with CALR and Cognition Disorders
Cardiovascular Diseases Antibodies	>3 publications with CALR and Cardiovascular Diseases
Brain Diseases Antibodies	>3 publications with CALR and Brain Diseases
Carcinoma, Hepatocellular Antibodies	>3 publications with CALR and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>3 publications with CALR and Liver Diseases

Organs/Tissues associated with anti-CALR antibody

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>4 publications with CALR and Eye
Heart Antibodies	>2 publications with CALR and Heart