Rabbit Anti-MEK 5 Polyclonal Antibody – Cat# MBS222783

MEK 5 Antibody – Rabbit Polyclonal

Name: MEK 5 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS222783
Price: 740 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS222783

MBS222783 specifically recognises 49kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315. MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MEK 5, Polyclonal Antibody

Full Product Name

RABBIT ANTI RAT MEK 5 (pSer311/Thr315)

Product Gene Name

anti-MEK 5 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from S

Form/Format

Purified
Purified IgG - liquid

Specificity

This item specifically recognises 49kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315.

MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the Ser311 and Thr315 sites has been implicated in cell proliferation.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.

Molecular Weight

40,091 Da

3D Structure

ModBase 3D Structure for Q62862

Perservative Stabilisers

0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
50% Glycerol

Immunogen

Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5, which includes phoshorylated Ser311 and Thr315.
Buffer Solution||10mM Hepes pH7.5

Antiserum Preparation

Antisera to MEK5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Target Species

Rat

Applications Tested/Suitable for anti-MEK 5 antibody

Western Blot (WB)*

Application Notes for anti-MEK 5 antibody

Western Blot: This item detects a band of approximately 49kDa in rat testes cell lysates.
Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Other Notes

Small volumes of anti-MEK 5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MEK 5 purchase

MBS222783 is an antibody targeting MEK 5. Also known as RABBIT ANTI RAT MEK 5 (pSer311/Thr315). Reactive in Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from S. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MEK 5. It may not necessarily be applicable to this product.

NCBI GI #

77157802

NCBI GeneID

29568

NCBI Accession #

NP_001029159.1

UniProt Primary Accession #

Q62862

UniProt Secondary Accession #

Q62863; Q62864

UniProt Related Accession #

Q62862

GenBank Nucleotide #

NM_001033987.1

NCBI Official Full Name

dual specificity mitogen-activated protein kinase kinase 5 isoform a

NCBI Official Synonym Full Names

mitogen activated protein kinase kinase 5

NCBI Official Symbol

Map2k5

NCBI Official Synonym Symbols

Mek5

UniProt Protein Name

Dual specificity mitogen-activated protein kinase kinase 5

UniProt Synonym Protein Names

MAPK/ERK kinase 5; MEK 5

Protein Family

Dual specificity mitogen-activated protein kinase kinase

UniProt Gene Name

Map2k5

UniProt Synonym Gene Names

Mek5; Mkk5; Prkmk5; MAP kinase kinase 5; MAPKK 5; MEK 5

UniProt Entry Name

MP2K5_RAT

NCBI Protein Information

dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPK/ERK kinase 5; MAPKK 5; MEK 5

NCBI Summary for MEK 5

kinase involved in signal transduction in the cell [RGD, Feb 2006]

UniProt Comments for MEK 5

MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; STE group; STE7 family

Cellular Component: membrane; spindle; intracellular; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; metal ion binding; protein-tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; negative regulation of heterotypic cell-cell adhesion; activation of MAPK activity; heart development; MAPKKK cascade; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of cell growth; protein amino acid phosphorylation; positive regulation of protein metabolic process; inhibition of NF-kappaB transcription factor; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation

Research Articles on MEK 5

1. The p38-mitogen-activated protein kinase activated protein kinase 2 (MK2) pathway is a component of the signaling cascade regulating oligodendrocyte differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MEK 5 antibody

Products by Pathway	Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
Gap Junction Pathway antibodies	Gap Junction Pathway Diagram
Gap Junction Pathway antibodies	Gap Junction Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Integrin-mediated Cell Adhesion Pathway antibodies	Integrin-mediated Cell Adhesion Pathway Diagram
MAPK (ERK5) Signaling Pathway antibodies	MAPK (ERK5) Signaling Pathway Diagram
MAPK (ERK5) Signaling Pathway antibodies	MAPK (ERK5) Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram

Diseases associated with anti-MEK 5 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>16 publications with MEK 5 and Nervous System Diseases
Body Weight Antibodies	>11 publications with MEK 5 and Body Weight
Hypertrophy Antibodies	>6 publications with MEK 5 and Hypertrophy
Kidney Diseases Antibodies	>5 publications with MEK 5 and Kidney Diseases
Cardiovascular Diseases Antibodies	>5 publications with MEK 5 and Cardiovascular Diseases
Necrosis Antibodies	>4 publications with MEK 5 and Necrosis
Inflammation Antibodies	>3 publications with MEK 5 and Inflammation
Hyperplasia Antibodies	>3 publications with MEK 5 and Hyperplasia
Heart Diseases Antibodies	>3 publications with MEK 5 and Heart Diseases
Brain Diseases Antibodies	>2 publications with MEK 5 and Brain Diseases

Organs/Tissues associated with anti-MEK 5 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>13 publications with MEK 5 and Brain
Muscle Antibodies	>11 publications with MEK 5 and Muscle
Prostate Antibodies	>7 publications with MEK 5 and Prostate
Lung Antibodies	>3 publications with MEK 5 and Lung
Artery Antibodies	>3 publications with MEK 5 and Artery
Connective Tissue Antibodies	>2 publications with MEK 5 and Connective Tissue
Embryonic Tissue Antibodies	>2 publications with MEK 5 and Embryonic Tissue
Eye Antibodies	>2 publications with MEK 5 and Eye