Rabbit Anti-5-HT7 Polyclonal Antibody – Cat# MBS222827

Staining of formalin fixed, paraffin embedded human cerebellum with Rabbit anti Human 5-HT7 receptor (MBS222827)

RECEPTOR (5-HT7) Antibody – Rabbit Polyclonal

Name: 5-HT7 Antibody; Polyclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS222827
Price: 775 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS222827

MBS222827 detects an epitope within the C-terminal of the serotonin (5-hydroxytryptamine, 5-HT) receptor 5-HT7, which belongs to the G-protein coupled receptor superfamily. Serotonin is a neurotransmitter and vasoactive amine which is thought to play a role… More details

$775 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

5-HT7 RECEPTOR, Polyclonal Antibody

Full Product Name

RABBIT ANTI HUMAN 5-HT7 RECEPTOR (C-TERMINAL)

Product Gene Name

anti-5-HT7 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse (Expected from Sequence)

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 1.0 mg/ml (lot specific)

Specificity

This item detects an epitope within the C-terminal of the serotonin (5-hydroxytryptamine, 5-HT) receptor 5-HT7, which belongs to the G-protein coupled receptor superfamily. Serotonin is a neurotransmitter and vasoactive amine which is thought to play a role in various cognitive and behavioural functions including pain, appetite, mood and sleep.

Signalling through 5-HT7 has several effects, including regulation of the circadian rhythm and mediation of smooth-muscle relaxation in the vasculature and gastrointestinal tract. Signalling though 5-HT7 has also been shown to contribute to early T-cell activation.

5-HT7 has been implicated in depression, autism, and other neuropsychiatric disorders.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

47,984 Da

OMIM

182137

3D Structure

ModBase 3D Structure for P34969

Perservative Stabilisers

0.1% Sodium Azide (NaN3)

Immunogen

Synthetic peptide C-terminal regoin of human 5-HT7 receptor, conjugated to Keyhole Limpet Hemocyanin (KLH).
Histology Positive Control Tissue||Brain, cerebellum
Buffer Solution||Phosphate buffered saline

Antiserum Preparation

Antisera to 5-HT7 receptor (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-5-HT7 antibody

Immunohistology Paraffin*

Application Notes for anti-5-HT7 antibody

Immunohistology - Paraffin: Maximum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Other Notes

Small volumes of anti-5-HT7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 5-HT7 purchase

MBS222827 is an antibody targeting 5-HT7. Also known as RABBIT ANTI HUMAN 5-HT7 RECEPTOR (C-TERMINAL). Reactive in Mouse (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for 5-HT7. It may not necessarily be applicable to this product.

NCBI GI #

4504547

NCBI GeneID

3363

NCBI Accession #

NP_000863.1

UniProt Primary Accession #

P34969

UniProt Secondary Accession #

P78336; P78372; P78516; Q5VX01; Q5VX02; Q5VX03; B5BUP6

UniProt Related Accession #

P34969

GenBank Nucleotide #

NM_000872.4

NCBI Official Full Name

5-hydroxytryptamine receptor 7 isoform a

NCBI Official Synonym Full Names

5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled

NCBI Official Symbol

HTR7

NCBI Official Synonym Symbols

5-HT7

UniProt Protein Name

5-hydroxytryptamine receptor 7

UniProt Synonym Protein Names

5-HT-X; Serotonin receptor 7

UniProt Gene Name

HTR7

UniProt Synonym Gene Names

5-HT-7; 5-HT7

UniProt Entry Name

5HT7R_HUMAN

NCBI Protein Information

5-hydroxytryptamine receptor 7; 5-HT-7; 5-HT-X; 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)

NCBI Summary for 5-HT7

The neurotransmitter, serotonin, is thought to play a role in various cognitive and behavioral functions. The serotonin receptor encoded by this gene belongs to the superfamily of G protein-coupled receptors and the gene is a candidate locus for involvement in autistic disorder and other neuropsychiatric disorders. Three splice variants have been identified which encode proteins that differ in the length of their carboxy terminal ends. [provided by RefSeq, Jul 2008]

UniProt Comments for 5-HT7

5-HT(7): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q21-q24

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: serotonin receptor activity

Biological Process: circadian rhythm; vasoconstriction; G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; smooth muscle contraction; blood circulation; serotonin receptor signaling pathway

Research Articles on 5-HT7

1. There is a cross-reactivity between 5-HT7 and 5-HT1A receptors and in this review they are shown to have a role in depressive disorders.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-5-HT7 antibody

Reference Product	PubMed Publications
HTR6 antibody	>8 publications with 5-HT7 and HTR6
HTR4 antibody	>6 publications with 5-HT7 and HTR4
DRD5 antibody	>1 publications with 5-HT7 and DRD5

Pathways associated with anti-5-HT7 antibody

Products by Pathway	Pathway Diagram
Amine Ligand-binding Receptors Pathway antibodies	Amine Ligand-binding Receptors Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram

Diseases associated with anti-5-HT7 antibody

Disease Name	Pubmed Publications
Mental Disorders Antibodies	>13 publications with 5-HT7 and Mental Disorders
Nervous System Diseases Antibodies	>6 publications with 5-HT7 and Nervous System Diseases
Inflammation Antibodies	>5 publications with 5-HT7 and Inflammation
Schizophrenia Antibodies	>5 publications with 5-HT7 and Schizophrenia
Disease Models, Animal Antibodies	>3 publications with 5-HT7 and Disease Models, Animal
Muscular Diseases Antibodies	>2 publications with 5-HT7 and Muscular Diseases
Pain Antibodies	>2 publications with 5-HT7 and Pain
Movement Disorders Antibodies	>1 publications with 5-HT7 and Movement Disorders
Anxiety Disorders Antibodies	>1 publications with 5-HT7 and Anxiety Disorders
Sleep Disorders Antibodies	>1 publications with 5-HT7 and Sleep Disorders

Organs/Tissues associated with anti-5-HT7 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>8 publications with 5-HT7 and Brain