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PDCD4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS222849

MBS222849 detects a specific epitope within the carboxy-terminal of programmed cell death 4 (Pdcd4), a novel tumour suppressor. Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation… More details

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Product Overview

Product Name
PDCD4, Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN PDCD4 (C-TERMINAL)
Product Gene Name
anti-PDCD4 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse (Expected from Sequence), Rat (Expected from Sequence), Xenopus (Expected from Sequence)
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
This item detects a specific epitope within the carboxy-terminal of programmed cell death 4 (Pdcd4), a novel tumour suppressor. Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis. Pdcd4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility.

Pdcd4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T cells. Pdcd4 expression is down regulated with tumour progression in carcinomas of the lung, colon, prostate and breast.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
50,576 Da
OMIM
608610
3D Structure
ModBase 3D Structure for Q53EL6
Perservative Stabilisers
0.01% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids near the carboxy terminal of human Pdcd4.
Histology Positive Control Tissue||Mouse colon.
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to Pdcd4 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-PDCD4 antibody
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-PDCD4 antibody
Western Blot: This item detects a band of approximately 52kDa.
ELISA: Minimum Dilution: 1/10000; Maximum Dilution: 1/50000
Immunohistology - Paraffin: Minimum Dilution: 1/100; Maximum Dilution: 1/300; Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000

Other Notes

Small volumes of anti-PDCD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDCD4 purchase

MBS222849 is an antibody targeting PDCD4. Also known as RABBIT ANTI HUMAN PDCD4 (C-TERMINAL). Reactive in Mouse (Expected from Sequence), Rat (Expected from Sequence), Xenopus (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDCD4. It may not necessarily be applicable to this product.

NCBI GI #
21735596
NCBI GeneID
27250
NCBI Accession #
NP_055271.2
UniProt Primary Accession #
Q53EL6
UniProt Secondary Accession #
O15501; Q5VZS6; Q6PJI5; Q8TAR5; Q99834; B2RCV4; B5ME91
UniProt Related Accession #
Q53EL6
GenBank Nucleotide #
NM_014456.4
NCBI Official Full Name
programmed cell death protein 4 isoform 1
NCBI Official Synonym Full Names
programmed cell death 4 (neoplastic transformation inhibitor)
NCBI Official Symbol
PDCD4
NCBI Official Synonym Symbols
H731
UniProt Protein Name
Programmed cell death protein 4
UniProt Synonym Protein Names
Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
Protein Family
Programmed cell death protein
UniProt Gene Name
PDCD4
UniProt Synonym Gene Names
H731
UniProt Entry Name
PDCD4_HUMAN
NCBI Protein Information
programmed cell death protein 4; neoplastic transformation inhibitor protein; nuclear antigen H731; protein 197/15a

NCBI Summary for PDCD4

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

UniProt Comments for PDCD4

PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: negative regulation of JNK activity; apoptosis; cell aging; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle
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Research Articles on PDCD4

1. When compared to controls, patients with ankylosing spondylitis had significantly higher levels of miR-21, PDCD4 mRNA, and CTX. MiR-21 expression was negatively correlated with PDCD4 in patients with AS who were taking neither NSAID nor DMARD.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDCD4 antibody

 Reference Product  PubMed Publications
 ERBB2 antibody  >5 publications with PDCD4 and ERBB2
 AKT1 antibody  >4 publications with PDCD4 and AKT1
 MAP4K1 antibody  >3 publications with PDCD4 and MAP4K1
 RPL5 antibody  >1 publications with PDCD4 and RPL5
 RPS13 antibody  >1 publications with PDCD4 and RPS13
 BTRC antibody  >1 publications with PDCD4 and BTRC
 EIF4A1 antibody  >1 publications with PDCD4 and EIF4A1

Pathways associated with anti-PDCD4 antibody

 Products by Pathway  Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram

Diseases associated with anti-PDCD4 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >42 publications with PDCD4 and Neoplasm Metastasis
 Cell Transformation, Neoplastic Antibodies  >42 publications with PDCD4 and Cell Transformation, Neoplastic
 Neoplasm Invasiveness Antibodies  >34 publications with PDCD4 and Neoplasm Invasiveness
 Inflammation Antibodies  >29 publications with PDCD4 and Inflammation
 Breast Neoplasms Antibodies  >29 publications with PDCD4 and Breast Neoplasms
 Lung Neoplasms Antibodies  >25 publications with PDCD4 and Lung Neoplasms
 Liver Diseases Antibodies  >25 publications with PDCD4 and Liver Diseases
 Liver Neoplasms Antibodies  >23 publications with PDCD4 and Liver Neoplasms
 Lung Diseases Antibodies  >22 publications with PDCD4 and Lung Diseases
 Neoplasms, Experimental Antibodies  >20 publications with PDCD4 and Neoplasms, Experimental

Organs/Tissues associated with anti-PDCD4 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >39 publications with PDCD4 and Lung
 Blood Antibodies  >38 publications with PDCD4 and Blood
 Liver Antibodies  >35 publications with PDCD4 and Liver
 Muscle Antibodies  >28 publications with PDCD4 and Muscle
 Brain Antibodies  >22 publications with PDCD4 and Brain
 Heart Antibodies  >19 publications with PDCD4 and Heart
 Skin Antibodies  >19 publications with PDCD4 and Skin
 Lymph Antibodies  >18 publications with PDCD4 and Lymph
 Lymph Node Antibodies  >18 publications with PDCD4 and Lymph Node
 Vascular Antibodies  >15 publications with PDCD4 and Vascular
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