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IGFBP-9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS222933

Rabbit anti Human IGFBP-9 antibody recognizes human IGFBP-9 (insulin-like growth factor binding protein- 9), also known as NOV/CCN3, a 326 amino acid cysteine-rich secreted regulatory protein and member of the CCN family, containing the domains IGFBP, VWFC,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IGFBP-9, Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN IGFBP-9
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - lyophilised
Concentration
IgG concentration 1.0mg/ml (lot specific)
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
39,162 Da
OMIM
164958
3D Structure
ModBase 3D Structure for P48745
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Immunogen
Recombinant human IGFBP-9
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antiserum to human IGFBP-9 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-IGFBP-9 antibody
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-IGFBP-9 antibody
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

Testing Data #1 of anti-IGFBP-9 antibody

Formalin fixed, paraffin embedded human breast invasive carcinoma stained with Rabbit anti Human IGFBP-9 (MBS222933) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Rabbit Anti-IGFBP-9 Polyclonal Antibody – Cat# MBS222933

Testing Data #2 of anti-IGFBP-9 antibody

Formalin fixed, paraffin embedded human breast invasive carcinoma stained with Rabbit anti Human IGFBP-9 (MBS222933) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Rabbit Anti-IGFBP-9 Polyclonal Antibody, Testing Data #2 – Cat# MBS222933

Other Notes

Small volumes of anti-IGFBP-9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IGFBP-9 purchase

MBS222933 is an antibody targeting IGFBP-9. Also known as RABBIT ANTI HUMAN IGFBP-9. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IGFBP-9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein NOV homolog
NCBI Official Synonym Full Names
nephroblastoma overexpressed
NCBI Official Symbol
NOV
NCBI Official Synonym Symbols
CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
UniProt Protein Name
Protein NOV homolog
UniProt Synonym Protein Names
CCN family member 3; Insulin-like growth factor-binding protein 9; IBP-9; IGF-binding protein 9; IGFBP-9; Nephroblastoma-overexpressed gene protein homolog
Protein Family
UniProt Gene Name
NOV
UniProt Synonym Gene Names
CCN3; IGFBP9; NOVH; NovH; IBP-9; IGF-binding protein 9; IGFBP-9
UniProt Entry Name
NOV_HUMAN
NCBI Protein Information
protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog

NCBI Summary for IGFBP-9

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]
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UniProt Comments for IGFBP-9

NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: proteinaceous extracellular matrix; cell soma; intracellular membrane-bound organelle; axon; dendrite

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; growth factor activity

Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; cell adhesion; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IGFBP-9 antibody

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