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AID Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223258

MBS221871 recognises an epitope within the C-terminal region (CT) of Activation-induced cytidine deaminase (AID), a 24kDa homologue of the RNA-editing cytidine deaminase enzyme, APOBEC-1. Although the molecular mechanisms by which AID acts remain unclear, a… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
AID, Polyclonal Antibody
Full Product Name
RABBIT ANTI AID (C-TERMINAL)
Product Gene Name
anti-AID antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
This item recognises an epitope within the C-terminal region (CT) of Activation-induced cytidine deaminase (AID), a 24kDa homologue of the RNA-editing cytidine deaminase enzyme, APOBEC-1.

Although the molecular mechanisms by which AID acts remain unclear, a growing number of studies reveal a requirement for AID during somatic hypermutation (SHM) and class switch recombination (CSR). These genetic modification events take place within highly specialised B cell germinal centres, during the final maturation stages of B cells, giving rise to high-affinity antibodies which use CSR to switch from IgM to IgG, IgA or IgE isotypes.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
22,614 Da
OMIM
605257
3D Structure
ModBase 3D Structure for Q9GZX7
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Preparation||Antisera to human AID (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG pepared by affinity chromatography.
Immunogen
A 14 amino acid peptide located near human AID carboxy-terminus.
Buffer Solution||Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-AID antibody
Western Blot (WB)
Application Notes for anti-AID antibody
Western Blot: This item detects a band of approximately 24kDa in Ramos whole cell lysates.
Western Blotting: Maximum Dilution: 2ug/ml

Testing Data #1 of anti-AID antibody

Western blot analysis of whole cel lysate from Ramos human Burkitt lymphomma cell line probed with Rabbit anti Human AID (MBS221871) in the absence (A) or presence (B) of blocking peptide
Rabbit Anti-AID Polyclonal Antibody – Cat# MBS223258

Testing Data #2 of anti-AID antibody

Immunocytochemical staining of Ramos cells with Rabbit anti Human AID (MBS221871)
Rabbit Anti-AID Polyclonal Antibody, Testing Data #2 – Cat# MBS223258

Other Notes

Small volumes of anti-AID antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AID purchase

MBS223258 is an antibody targeting AID. Also known as RABBIT ANTI AID (C-TERMINAL). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AID. It may not necessarily be applicable to this product.

NCBI GI #
10190700
NCBI GeneID
57379
NCBI Accession #
NP_065712.1
UniProt Primary Accession #
Q9GZX7
UniProt Secondary Accession #
Q6QJ81; Q8NFC1
UniProt Related Accession #
Q9GZX7
GenBank Nucleotide #
NM_020661.2
NCBI Official Full Name
single-stranded DNA cytosine deaminase
NCBI Official Synonym Full Names
activation-induced cytidine deaminase
NCBI Official Symbol
AICDA
NCBI Official Synonym Symbols
AID; ARP2; CDA2; HIGM2; HEL-S-284
UniProt Protein Name
Single-stranded DNA cytosine deaminase
UniProt Synonym Protein Names
Activation-induced cytidine deaminase; Cytidine aminohydrolase
Protein Family
AIDA-I autotransporter
UniProt Gene Name
AICDA
UniProt Synonym Gene Names
AID
UniProt Entry Name
AICDA_HUMAN
NCBI Protein Information
single-stranded DNA cytosine deaminase; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos

NCBI Summary for AID

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]
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UniProt Comments for AID

AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: EC 3.5.4.38; Hydrolase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasm; exosome (RNase complex); nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; cytidine deaminase activity

Biological Process: somatic diversification of immunoglobulins; B cell differentiation; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing

Disease: Immunodeficiency With Hyper-igm, Type 2
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Research Articles on AID

1. data demonstrate that AID is active in CLL in vivo and thus, AID likely contributes to clonal evolution of CLL.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AID antibody

 Reference Product  PubMed Publications
 TNFSF13B antibody  >6 publications with AID and TNFSF13B
 STAT6 antibody  >5 publications with AID and STAT6
 IL4 antibody  >3 publications with AID and IL4

Pathways associated with anti-AID antibody

 Products by Pathway  Pathway Diagram
 IL4-mediated Signaling Events Pathway antibodies  IL4-mediated Signaling Events Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram

Diseases associated with anti-AID antibody

 Disease Name  Pubmed Publications
 Leukemia Antibodies  >65 publications with AID and Leukemia
 Leukemia, Lymphoid Antibodies  >46 publications with AID and Leukemia, Lymphoid
 Inflammation Antibodies  >38 publications with AID and Inflammation
 Autoimmune Diseases Antibodies  >38 publications with AID and Autoimmune Diseases
 Necrosis Antibodies  >28 publications with AID and Necrosis
 Disease Models, Animal Antibodies  >26 publications with AID and Disease Models, Animal
 Hyper-IgM Immunodeficiency Syndrome Antibodies  >19 publications with AID and Hyper-IgM Immunodeficiency Syndrome
 Liver Diseases Antibodies  >18 publications with AID and Liver Diseases
 Adenocarcinoma Antibodies  >14 publications with AID and Adenocarcinoma
 Nervous System Diseases Antibodies  >12 publications with AID and Nervous System Diseases

Organs/Tissues associated with anti-AID antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >462 publications with AID and Blood
 Lymph Node Antibodies  >100 publications with AID and Lymph Node
 Lymph Antibodies  >26 publications with AID and Lymph
 Kidney Antibodies  >26 publications with AID and Kidney
 Tonsil Antibodies  >12 publications with AID and Tonsil
 Pharynx Antibodies  >8 publications with AID and Pharynx
 Testis Antibodies  >2 publications with AID and Testis
 Pancreas Antibodies  >1 publications with AID and Pancreas
 Uncharacterized Tissue Antibodies  >1 publications with AID and Uncharacterized Tissue
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