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CONNEXIN 43 (GJA1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223268

MBS223268 specifically recognises 43kDa Connexin43, when phosphorylated at Ser368. Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and… More details

$775 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CONNEXIN 43 (Gja1), Polyclonal Antibody
Full Product Name
RABBIT ANTI CONNEXIN 43 (pSer368)
Product Gene Name
anti-Gja1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Guinea Pig (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Zebrafish (Expected from Sequence)
Form/Format
Purified
Purified IgG - liquid
Specificity
This item specifically recognises 43kDa Connexin43, when phosphorylated at Ser368. Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. Studies suggest that a decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of CX43, and that this is mediated by the phosphorylation of CX43 at residue Ser368, by Protein Kinase C (PKC). A possible link between CX43 and epilepsy has been investigated following the detection of high levels of CX43 in both low-grade tumours and peritumoural reactive astrocytes, see Aronica, E. et al. for details.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
Molecular Weight
43,031 Da
3D Structure
ModBase 3D Structure for P08050
Perservative Stabilisers
0.09% Sodium Azide
0.01% Bovine Serum Albumin
50% Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within connexin43 which includes phosphorylated Ser368
Buffer Solution||10mM HEPES pH7.5
Antiserum Preparation
Antisera to rat connexin43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Rat
Applications Tested/Suitable for anti-Gja1 antibody
Western Blot (WB)*
Application Notes for anti-Gja1 antibody
Western Blot: This item detects a band of approximately 43kDa in rat hippocampus cell lysates.
Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Other Notes

Small volumes of anti-Gja1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Gja1 purchase

MBS223268 is an antibody targeting Gja1. Also known as RABBIT ANTI CONNEXIN 43 (pSer368). Reactive in Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Guinea Pig (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Zebrafish (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Gja1. It may not necessarily be applicable to this product.

NCBI GI #
6978896
NCBI GeneID
24392
NCBI Accession #
NP_036699.1
UniProt Primary Accession #
P08050
UniProt Secondary Accession #
Q53ZE1
UniProt Related Accession #
P08050
GenBank Nucleotide #
NM_012567.2
NCBI Official Full Name
gap junction alpha-1 protein
NCBI Official Synonym Full Names
gap junction protein, alpha 1
NCBI Official Symbol
Gja1
NCBI Official Synonym Symbols
Cx43
UniProt Protein Name
Gap junction alpha-1 protein
UniProt Synonym Protein Names
Connexin-43; Cx43; Gap junction 43 kDa heart protein
Protein Family
Gap junction alpha-1 protein
UniProt Gene Name
Gja1
UniProt Synonym Gene Names
Cxn-43; Cx43
UniProt Entry Name
CXA1_RAT
NCBI Protein Information
gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1

NCBI Summary for Gja1

gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]

UniProt Comments for Gja1

GJA1: an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Channel, misc.; Membrane protein, integral

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; focal adhesion; contractile fiber; lysosome; integral to plasma membrane; early endosome; intermediate filament; fascia adherens; intercellular junction; cytosol; lipid raft; Golgi membrane; connexon complex; multivesicular body; mitochondrial outer membrane; membrane; cytoplasm; apical plasma membrane; late endosome; plasma membrane; gap junction; cell junction; endosome; lateral plasma membrane

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; ion transmembrane transporter activity; beta-tubulin binding; SH3 domain binding; gap junction channel activity; receptor binding; PDZ domain binding

Biological Process: lens development in camera-type eye; response to peptide hormone stimulus; apoptosis; heart development; neuron migration; milk ejection; positive regulation of vasodilation; signal transduction; regulation of heart contraction; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; cell-cell signaling; positive regulation of glomerular filtration; negative regulation of cardiac muscle cell proliferation; response to glucose stimulus; positive regulation of striated muscle development; cell communication; heart looping; transmembrane transport; ATP transport; adult heart development; intercellular junction assembly and maintenance; chronic inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; gap junction assembly; epithelial cell maturation; in utero embryonic development; regulation of bone remodeling; positive regulation of insulin secretion; skeletal muscle regeneration; regulation of calcium ion transport; vascular transport; protein oligomerization; osteoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of endothelial cell proliferation; positive regulation of vasoconstriction; positive regulation of protein catabolic process; embryonic heart tube development; blood vessel morphogenesis; embryonic digit morphogenesis; regulation of bone mineralization; neurite morphogenesis; response to pH
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Research Articles on Gja1

1. Internal ribosomal entry site (IRES) activity generates endogenous carboxyl-terminal domains of Cx43 and is responsive to hypoxic conditions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Gja1 antibody

 Products by Pathway  Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Formation Of Annular Gap Junctions Pathway antibodies  Formation Of Annular Gap Junctions Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Assembly Pathway antibodies  Gap Junction Assembly Pathway Diagram
 Gap Junction Degradation Pathway antibodies  Gap Junction Degradation Pathway Diagram
 Gap Junction Trafficking Pathway antibodies  Gap Junction Trafficking Pathway Diagram

Diseases associated with anti-Gja1 antibody

 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >72 publications with Gja1 and Heart Diseases
 Oculodentodigital Dysplasia Antibodies  >60 publications with Gja1 and Oculodentodigital Dysplasia
 Arrhythmias, Cardiac Antibodies  >44 publications with Gja1 and Arrhythmias, Cardiac
 Disease Models, Animal Antibodies  >34 publications with Gja1 and Disease Models, Animal
 Fibrosis Antibodies  >13 publications with Gja1 and Fibrosis
 Inflammation Antibodies  >13 publications with Gja1 and Inflammation
 Necrosis Antibodies  >13 publications with Gja1 and Necrosis
 Lung Neoplasms Antibodies  >11 publications with Gja1 and Lung Neoplasms
 Infertility, Male Antibodies  >10 publications with Gja1 and Infertility, Male
 Adenocarcinoma Antibodies  >9 publications with Gja1 and Adenocarcinoma

Organs/Tissues associated with anti-Gja1 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >112 publications with Gja1 and Heart
 Muscle Antibodies  >106 publications with Gja1 and Muscle
 Eye Antibodies  >65 publications with Gja1 and Eye
 Brain Antibodies  >38 publications with Gja1 and Brain
 Ovary Antibodies  >23 publications with Gja1 and Ovary
 Embryonic Tissue Antibodies  >18 publications with Gja1 and Embryonic Tissue
 Lung Antibodies  >17 publications with Gja1 and Lung
 Kidney Antibodies  >11 publications with Gja1 and Kidney
 Connective Tissue Antibodies  >9 publications with Gja1 and Connective Tissue
 Liver Antibodies  >8 publications with Gja1 and Liver
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