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DNASE II (DNASE2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223331

MBS223331 recognises human DNase II (Deoxyribonuclease II), an enzyme involved in both the condensation of chromatin and the fragmentation of DNA. DNase II is ubiquitously expressed in human tissues. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
DNASE II (DNASE2), Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN DNASE II (C-TERMINAL)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
This item recognises human DNase II (Deoxyribonuclease II), an enzyme involved in both the condensation of chromatin and the fragmentation of DNA.

DNase II is ubiquitously expressed in human tissues.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
33,615 Da
OMIM
126350
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids near the Carboxy terminus of human DNase II precursor.
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antiserum to human DNase II (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-DNASE2 antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-DNASE2 antibody
Western Blot: This item detects a band of approximately 40kDa in human tissue spleen cell lysates.
Immunohistology - Paraffin: Maximum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml

Testing Data #1 of anti-DNASE2 antibody

Western blot analysis of human spleen lysate probed with Rabbit anti Human DNASE-II (MBS223331) either in the absence (1) or presence (2) of blocking peptide
Rabbit Anti-DNASE2 Polyclonal Antibody – Cat# MBS223331

Testing Data #2 of anti-DNASE2 antibody

Immunohistochemical staining of human spleen with Rabbit anti DNASE-II (MBS223331)
Rabbit Anti-DNASE2 Polyclonal Antibody, Testing Data #2 – Cat# MBS223331

Other Notes

Small volumes of anti-DNASE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNASE2 purchase

MBS223331 is an antibody targeting DNASE2. Also known as RABBIT ANTI HUMAN DNASE II (C-TERMINAL). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DNASE2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
deoxyribonuclease-2-alpha
NCBI Official Synonym Full Names
deoxyribonuclease II, lysosomal
NCBI Official Symbol
DNASE2
NCBI Official Synonym Symbols
DNL; DNL2; DNASE2A
UniProt Protein Name
Deoxyribonuclease-2-alpha
UniProt Synonym Protein Names
Acid DNase; Deoxyribonuclease II alpha; DNase II alpha; Lysosomal DNase II; R31240_2
Protein Family
UniProt Gene Name
DNASE2
UniProt Synonym Gene Names
DNASE2A; DNL2; DNase II alpha
UniProt Entry Name
DNS2A_HUMAN
NCBI Protein Information
deoxyribonuclease-2-alpha; DNase II alpha; DNase II, lysosomal; R31240_2; acid DNase; deoxyribonuclease II alpha; lysosomal DNase II

NCBI Summary for DNASE2

This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008]
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UniProt Comments for DNASE2

DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family.

Protein type: Deoxyribonuclease; EC 3.1.22.1

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; intracellular

Molecular Function: deoxyribonuclease II activity; DNA binding

Biological Process: erythrocyte differentiation; DNA fragmentation during apoptosis; DNA metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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