Rabbit Anti-PAI-1 Polyclonal Antibody – Cat# MBS223369

Formalin fixed human breast invasive ductal carcinoma stained with Rabbit anti human PAI-1 (MBS223369) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

PAI-1 Antibody – Rabbit Polyclonal

Name: PAI-1 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS223369
Price: 740 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223369

MBS223369 specifically recognises human PAI-1 (plasminogen activator inhibitor-1), otherwise known as SERPINE1, a 45kDa serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and… More details

$740 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PAI-1, Polyclonal Antibody

Full Product Name

RABBIT ANTI HUMAN PAI-1

Product Gene Name

anti-PAI-1 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Form/Format

Purified
Purified IgG - lyophilised

Concentration

IgG concentration 1.0mg/ml (lot specific)

Specificity

This item specifically recognises human PAI-1 (plasminogen activator inhibitor-1), otherwise known as SERPINE1, a 45kDa serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis.

Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and VLDL (very-low-density lipoprotein).

Preparation and Storage

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

Molecular Weight

43,404 Da

OMIM

173360

3D Structure

ModBase 3D Structure for P05121

Reconstitution

Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.

Immunogen

Recombinant human PAI-1
Buffer Solution||Phosphate buffered saline

Antiserum Preparation

Antiserum to human PAI-1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-PAI-1 antibody

ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)

Application Notes for anti-PAI-1 antibody

ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

Other Notes

Small volumes of anti-PAI-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAI-1 purchase

MBS223369 is an antibody targeting PAI-1. Also known as RABBIT ANTI HUMAN PAI-1. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAI-1. It may not necessarily be applicable to this product.

NCBI GI #

10835159

NCBI GeneID

5054

NCBI Accession #

NP_000593.1

UniProt Primary Accession #

P05121

UniProt Secondary Accession #

B7Z4S0; F8WD53

UniProt Related Accession #

P05121

GenBank Nucleotide #

NM_000602.4

NCBI Official Full Name

plasminogen activator inhibitor 1

NCBI Official Synonym Full Names

serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

NCBI Official Symbol

SERPINE1

NCBI Official Synonym Symbols

PAI; PAI1; PAI-1; PLANH1

UniProt Protein Name

Plasminogen activator inhibitor 1

UniProt Synonym Protein Names

Endothelial plasminogen activator inhibitor; Serpin E1

Protein Family

Plasminogen activator inhibitor

UniProt Gene Name

SERPINE1

UniProt Synonym Gene Names

PAI1; PLANH1; PAI; PAI-1

UniProt Entry Name

PAI1_HUMAN

NCBI Protein Information

plasminogen activator inhibitor 1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1

NCBI Summary for PAI-1

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

UniProt Comments for PAI-1

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; negative regulation of fibrinolysis; platelet degranulation; transforming growth factor beta receptor signaling pathway; angiogenesis; regulation of receptor activity; chronological cell aging; negative regulation of cell migration; negative regulation of cell adhesion mediated by integrin; platelet activation; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of blood coagulation; regulation of cell proliferation; fibrinolysis; positive regulation of angiogenesis; gene expression; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency

Research Articles on PAI-1

1. The carriers of polymorphism 4G are at greater risk for early pregnancy loss than those with 5G, which confirms that carriage of the polymorphism 4G/5G 675 gene PAI-1 has a specific in pathogenesis and expression of Polycystic Ovary Syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAI-1 antibody

Reference Product	PubMed Publications
TGFB1 antibody	>531 publications with PAI-1 and TGFB1
SMAD4 antibody	>82 publications with PAI-1 and SMAD4
HIF1A antibody	>66 publications with PAI-1 and HIF1A
EGF antibody	>63 publications with PAI-1 and EGF
PLG antibody	>43 publications with PAI-1 and PLG
PLAT antibody	>24 publications with PAI-1 and PLAT
PLAU antibody	>13 publications with PAI-1 and PLAU
VTN antibody	>5 publications with PAI-1 and VTN
ALB antibody	>3 publications with PAI-1 and ALB

Pathways associated with anti-PAI-1 antibody

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway antibodies	BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway Diagram
Blood Clotting Cascade Pathway antibodies	Blood Clotting Cascade Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Circadian Clock Pathway antibodies	Circadian Clock Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram

Diseases associated with anti-PAI-1 antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>3215 publications with PAI-1 and Cardiovascular Diseases
Thrombosis Antibodies	>1406 publications with PAI-1 and Thrombosis
Inflammation Antibodies	>1333 publications with PAI-1 and Inflammation
Heart Diseases Antibodies	>1055 publications with PAI-1 and Heart Diseases
Necrosis Antibodies	>896 publications with PAI-1 and Necrosis
Obesity Antibodies	>883 publications with PAI-1 and Obesity
Atherosclerosis Antibodies	>781 publications with PAI-1 and Atherosclerosis
Hypertension Antibodies	>703 publications with PAI-1 and Hypertension
Fibrosis Antibodies	>695 publications with PAI-1 and Fibrosis
Disease Models, Animal Antibodies	>542 publications with PAI-1 and Disease Models, Animal

Organs/Tissues associated with anti-PAI-1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5356 publications with PAI-1 and Blood
Vascular Antibodies	>2842 publications with PAI-1 and Vascular
Heart Antibodies	>940 publications with PAI-1 and Heart
Kidney Antibodies	>813 publications with PAI-1 and Kidney
Muscle Antibodies	>741 publications with PAI-1 and Muscle
Liver Antibodies	>620 publications with PAI-1 and Liver
Connective Tissue Antibodies	>589 publications with PAI-1 and Connective Tissue
Lung Antibodies	>584 publications with PAI-1 and Lung
Adipose Tissue Antibodies	>525 publications with PAI-1 and Adipose Tissue
Brain Antibodies	>401 publications with PAI-1 and Brain