• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CCL25 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223592

Rabbit anti Human CCL25 antibody specifically recognizes human CCL25, otherwise known as TECK (thymus expressed chemokine), a member of the intercrine beta (chemokine CC) family, specifically expressed by thymic stromal cells, which acts as a chemotactic… More details

$740 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CCL25, Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN CCL25
Product Gene Name
anti-CCL25 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - lyophilised
Concentration
IgG concentration 1.0mg/ml after reconstitution (lot specific)
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
16,538 Da
OMIM
602565
3D Structure
ModBase 3D Structure for O15444
Reconstitution
Reconstitute with 0.1ml distilled water.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Immunogen
Recombinant human CCL25.
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antiserum to human CCL25 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-CCL25 antibody
ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-CCL25 antibody
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;
Functional Assays: Minimum Dilution: 10.0; Maximum Dilution: 15.0ug/ml;
Immunohistology - Paraffin: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml; Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

Testing Data #1 of anti-CCL25 antibody

Formalin fixed, paraffin embedded normal human small bowel stained with Rabbit anti Human CCL25 (MBS223592) followiing antigen retrieval with proteinase k
Rabbit Anti-CCL25 Polyclonal Antibody – Cat# MBS223592

Testing Data #2 of anti-CCL25 antibody

Formalin fixed, paraffin embedded normal human small bowel stained with Rabbit anti Human CCL25 (MBS223592) followiing antigen retrieval with proteinase k
Rabbit Anti-CCL25 Polyclonal Antibody, Testing Data #2 – Cat# MBS223592

Other Notes

Small volumes of anti-CCL25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL25 purchase

MBS223592 is an antibody targeting CCL25. Also known as RABBIT ANTI HUMAN CCL25. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL25. It may not necessarily be applicable to this product.

NCBI GI #
323098324
NCBI GeneID
6370
NCBI Accession #
NP_001188288.1
UniProt Primary Accession #
O15444
UniProt Secondary Accession #
Q96KJ7; A1L4J4; A6NI52; A8K9E7; B5MCA5
UniProt Related Accession #
O15444
GenBank Nucleotide #
NM_001201359.1
NCBI Official Full Name
C-C motif chemokine 25 isoform 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 25
NCBI Official Symbol
CCL25
NCBI Official Synonym Symbols
TECK; Ckb15; SCYA25
UniProt Protein Name
C-C motif chemokine 25
UniProt Synonym Protein Names
Chemokine TECK; Small-inducible cytokine A25; Thymus-expressed chemokine
Protein Family
C-C motif chemokine
UniProt Gene Name
CCL25
UniProt Synonym Gene Names
SCYA25; TECK
UniProt Entry Name
CCL25_HUMAN
NCBI Protein Information
C-C motif chemokine 25; Ck beta-15; TECKvar; chemokine TECK; small inducible cytokine subfamily A (Cys-Cys), member 25; small-inducible cytokine A25; thymus expressed chemokine; thymus-expressed chemokine

NCBI Summary for CCL25

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Show more

UniProt Comments for CCL25

CCL25: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Belongs to the intercrine beta (chemokine CC) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; immune response; positive regulation of cell-matrix adhesion; inflammatory response; chemotaxis; leukocyte migration
Show more

Research Articles on CCL25

1. CCL25 is an antimicrobial protein with bacteriocidal activity against E. coli and S. aureus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CCL25 antibody

 Reference Product  PubMed Publications
 CCR9 antibody  >138 publications with CCL25 and CCR9
 CCL19 antibody  >30 publications with CCL25 and CCL19
 CCL20 antibody  >19 publications with CCL25 and CCL20
 CCR7 antibody  >19 publications with CCL25 and CCR7
 CXCL13 antibody  >12 publications with CCL25 and CXCL13
 CCR6 antibody  >11 publications with CCL25 and CCR6
 CCR5 antibody  >11 publications with CCL25 and CCR5
 CXCL9 antibody  >5 publications with CCL25 and CXCL9
 CCL27 antibody  >5 publications with CCL25 and CCL27
 CXCR5 antibody  >4 publications with CCL25 and CXCR5

Pathways associated with anti-CCL25 antibody

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram

Diseases associated with anti-CCL25 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >54 publications with CCL25 and Inflammation
 Gastrointestinal Diseases Antibodies  >37 publications with CCL25 and Gastrointestinal Diseases
 Immune System Diseases Antibodies  >29 publications with CCL25 and Immune System Diseases
 Necrosis Antibodies  >24 publications with CCL25 and Necrosis
 Liver Diseases Antibodies  >10 publications with CCL25 and Liver Diseases
 Carcinoma Antibodies  >8 publications with CCL25 and Carcinoma
 Lymphoma Antibodies  >7 publications with CCL25 and Lymphoma
 Atrophy Antibodies  >5 publications with CCL25 and Atrophy
 Neoplasms, Experimental Antibodies  >4 publications with CCL25 and Neoplasms, Experimental
 Graft vs Host Disease Antibodies  >3 publications with CCL25 and Graft vs Host Disease

Organs/Tissues associated with anti-CCL25 antibody

 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >84 publications with CCL25 and Thymus
 Lung Antibodies  >9 publications with CCL25 and Lung
 Stomach Antibodies  >5 publications with CCL25 and Stomach
 Mixed Antibodies  >2 publications with CCL25 and Mixed
 Testis Antibodies  >1 publications with CCL25 and Testis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000