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PLATELET FACTOR-4 (PF4) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223824

Rabbit anti Human Platelet Factor-4 antibody specifically recognizes platelet factor-4 (PF-4), otherwise known as CXCL4, a member of the intercrine alpha (chemokine CXC) family, released from the alpha granules of activated platelets, during platelet… More details

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Testing Data View PDF
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Product Overview

Product Name
PLATELET FACTOR-4 (PF4), Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN PLATELET FACTOR-4
Product Gene Name
anti-PF4 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - lyophilised
Concentration
IgG concentration 1.0mg/ml after reconstitution (lot specific)
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
10,845 Da
OMIM
173460
3D Structure
ModBase 3D Structure for P02776
Reconstitution
Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Immunogen
Recombinant human PF-4
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antiserum to human PF-4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-PF4 antibody
ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-PF4 antibody
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;
Functional Assays: Minimum Dilution: 1.75; Maximum Dilution: 3.0ug/ml;
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

Testing Data #1 of anti-PF4 antibody

Formalin fixed, paraffin embedded human breast malignant ductal adenocarcinoma stained with Rabbit anti Human platelet factor 4 (MBS223824) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0) using an alkaline phosphatase conjugated polymer detection system and red chromogen
Rabbit Anti-PF4 Polyclonal Antibody – Cat# MBS223824

Testing Data #2 of anti-PF4 antibody

Formalin fixed, paraffin embedded human breast malignant ductal adenocarcinoma stained with Rabbit anti Human platelet factor 4 (MBS223824) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0) using an alkaline phosphatase conjugated polymer detection system and red chromogen
Rabbit Anti-PF4 Polyclonal Antibody, Testing Data #2 – Cat# MBS223824

Testing Data #3 of anti-PF4 antibody

Formalin fixed, paraffin embedded human breast malignant ductal adenocarcinoma stained with Rabbit anti Human platelet factor 4 (MBS223824) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0) using an alkaline phosphatase conjugated polymer detection system and red chromogen
Rabbit Anti-PF4 Polyclonal Antibody, Testing Data #3 – Cat# MBS223824

Other Notes

Small volumes of anti-PF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PF4 purchase

MBS223824 is an antibody targeting PF4. Also known as RABBIT ANTI HUMAN PLATELET FACTOR-4. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PF4. It may not necessarily be applicable to this product.

NCBI GI #
4505733
NCBI GeneID
5196
NCBI Accession #
NP_002610.1
UniProt Primary Accession #
P02776
UniProt Secondary Accession #
Q53X61; Q9UC64; Q9UC65
UniProt Related Accession #
P02776
GenBank Nucleotide #
NM_002619.3
NCBI Official Full Name
platelet factor 4
NCBI Official Synonym Full Names
platelet factor 4
NCBI Official Symbol
PF4
NCBI Official Synonym Symbols
PF-4; CXCL4; SCYB4
UniProt Protein Name
Platelet factor 4
UniProt Synonym Protein Names
C-X-C motif chemokine 4; Iroplact; Oncostatin-APlatelet factor 4, short form
Protein Family
Platelet factor
UniProt Gene Name
PF4
UniProt Synonym Gene Names
CXCL4; SCYB4; PF-4
UniProt Entry Name
PLF4_HUMAN
NCBI Protein Information
platelet factor 4; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; iroplact; oncostatin-A

NCBI Summary for PF4

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014]
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UniProt Comments for PF4

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Chemokine; Apoptosis; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q12-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; platelet degranulation; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of macrophage differentiation; blood coagulation; inflammatory response
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Research Articles on PF4

1. CXCL4 and CXCL4L1 activated p38 MAPK, as well as Src kinase within 30 and 5 min, respectively. Extracellular signal-regulated kinase (ERK) phosphorylation occurred in activated lymphocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PF4 antibody

 Reference Product  PubMed Publications
 FGF2 antibody  >16 publications with PF4 and FGF2
 CXCR3 antibody  >14 publications with PF4 and CXCR3
 PROC antibody  >12 publications with PF4 and PROC
 CXCL11 antibody  >6 publications with PF4 and CXCL11

Pathways associated with anti-PF4 antibody

 Products by Pathway  Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram

Diseases associated with anti-PF4 antibody

 Disease Name  Pubmed Publications
 Thrombocytopenia Antibodies  >487 publications with PF4 and Thrombocytopenia
 Cardiovascular Diseases Antibodies  >369 publications with PF4 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >346 publications with PF4 and Vascular Diseases
 Thrombosis Antibodies  >319 publications with PF4 and Thrombosis
 Heart Diseases Antibodies  >120 publications with PF4 and Heart Diseases
 Inflammation Antibodies  >79 publications with PF4 and Inflammation
 Nervous System Diseases Antibodies  >67 publications with PF4 and Nervous System Diseases
 Autoimmune Diseases Antibodies  >59 publications with PF4 and Autoimmune Diseases
 Kidney Diseases Antibodies  >57 publications with PF4 and Kidney Diseases
 Atherosclerosis Antibodies  >55 publications with PF4 and Atherosclerosis

Organs/Tissues associated with anti-PF4 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >106 publications with PF4 and Heart
 Bone Antibodies  >80 publications with PF4 and Bone
 Bone Marrow Antibodies  >71 publications with PF4 and Bone Marrow
 Skin Antibodies  >35 publications with PF4 and Skin
 Lung Antibodies  >33 publications with PF4 and Lung
 Embryonic Tissue Antibodies  >18 publications with PF4 and Embryonic Tissue
 Intestine Antibodies  >7 publications with PF4 and Intestine
 Placenta Antibodies  >1 publications with PF4 and Placenta
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