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ATG12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223848

Rabbit anti ATG12 (N-Terminal) antibody recognizes human Autophagy-related protein 12 (ATG12). ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
ATG12, Polyclonal Antibody
Full Product Name
RABBIT ANTI ATG12 (N-TERMINAL)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
15,284 Da
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a 16 aa sequence from near the amino terminus of human ATG12.
Histology Positive Control Tissue||Human brain
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antiserum to human ATG12 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-ATG12 antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-ATG12 antibody
Western Blot: This item detects a band of approximately 40 kDa in human brain tissue lysate.
Immunohistology - Paraffin: Minimum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1ug/ml

Testing Data #1 of anti-ATG12 antibody

Western blot analysis of human brain lysate probed with Rabbit anti Human ATG12 (MBS223848) at 0.5(A) and 1(B) ug/ml
Rabbit Anti-ATG12 Polyclonal Antibody – Cat# MBS223848

Testing Data #2 of anti-ATG12 antibody

Immunohistochemical staining of human brain with Rabbit anti Human ATG12 (MBS223848)
Rabbit Anti-ATG12 Polyclonal Antibody, Testing Data #2 – Cat# MBS223848

Other Notes

Small volumes of anti-ATG12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG12 purchase

MBS223848 is an antibody targeting ATG12. Also known as RABBIT ANTI ATG12 (N-TERMINAL). Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-like protein ATG12
NCBI Official Synonym Full Names
autophagy related 12
NCBI Official Symbol
Atg12
NCBI Official Synonym Symbols
Apg12l
UniProt Protein Name
Ubiquitin-like protein ATG12
UniProt Synonym Protein Names
Autophagy-related protein 12; APG12-like
Protein Family
UniProt Gene Name
Atg12
UniProt Synonym Gene Names
Apg12l; APG12-like
UniProt Entry Name
ATG12_RAT
NCBI Protein Information
ubiquitin-like protein ATG12; ATG12 autophagy related 12 homolog; autophagy-related 12; autophagy-related protein 12

UniProt Comments for ATG12

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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