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Endothelin B RECEPTOR (EDNRB) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223880

Sheep anti Rat endothelin B receptor antibody recognizes an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ENDOTHELIN B RECEPTOR (Ednrb), Polyclonal Antibody
Full Product Name
SHEEP ANTI RAT ENDOTHELIN B RECEPTOR (C-TERMINAL)
Product Gene Name
anti-Ednrb antibody
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Human, Mouse
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.41mg/ml (lot specific)
Specificity
This item specifically recognises an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system. This item recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody. This item does not block the binding of endothelins. This item is reported as suitable for use in immunocytochemistry on acetone fixed cells.
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Perservative Stabilizers
<0.1% Sodium Azide (NaN3)
Immunogen
Keyhole limpet haemocyanin (KLH) conjugated C-terminal peptide KANDHGYDNFRSSNN corresponding to amino acids 424 to 437 of rat endothelin B receptor.
Buffer Solution
Phosphate buffered saline
Antiserum Preparation
Antiserum to rat ET-B (CT) was raised by repeated immunization of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Rat
Species Cross-Reactivity
Mouse, Human
N.B: Antibody reactivity and working conditions may vary between species. Cross reactivity is dervied from testing within the QC internal labs, peer reviewed publications or personal communications from the originators.
Applications Tested/Suitable for anti-Ednrb antibody
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin
Application Notes for anti-Ednrb antibody
Immunofluorescence: 10-20ug/ml;
Immunoprecipitation: 20-40ug/ml;
Immunohistology - Paraffin: 10-20ug/ml

Where this product has not been tested for use in a partivular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Other Notes

Small volumes of anti-Ednrb antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ednrb purchase

MBS223880 is an antibody targeting Ednrb. Also known as SHEEP ANTI RAT ENDOTHELIN B RECEPTOR (C-TERMINAL). Reactive in Human, Mouse. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for Ednrb. It may not necessarily be applicable to this product.

NCBI GI #
8393333
NCBI GeneID
50672
NCBI Accession #
NP_059029.1
UniProt Related Accession #
P21451
GenBank Nucleotide #
NM_017333.1
NCBI Official Full Name
endothelin B receptor
NCBI Official Synonym Full Names
endothelin receptor type B
NCBI Official Symbol
Ednrb
NCBI Official Synonym Symbols
Etb; Ednra
UniProt Protein Name
Endothelin B receptor
UniProt Synonym Protein Names
Endothelin receptor non-selective type
Protein Family
Endothelin B receptor
UniProt Gene Name
Ednrb
UniProt Synonym Gene Names
ET-B; ET-BR
UniProt Entry Name
EDNRB_RAT
NCBI Protein Information
endothelin B receptor; ET-B; ET-BR; endothelin receptor non-selective type; endothelin-B receptor

UniProt Comments for Ednrb

ETB: a G protein-coupled receptor which activates a phosphatidylinoitol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutation in endothelin receptor type B gene. Essential component in the normal development of two neuronal crest-derived cell lineages. Two splice variant isoforms have been described.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Cellular Component: nuclear membrane; membrane; integral to membrane; plasma membrane; lipid raft

Molecular Function: endothelin receptor activity; G-protein coupled receptor activity; peptide hormone binding; receptor activity; type 1 angiotensin receptor binding

Biological Process: epithelial fluid transport; cGMP-mediated signaling; pigmentation during development; regulation of fever; negative regulation of cellular protein metabolic process; response to lipopolysaccharide; response to pain; negative regulation of transcription from RNA polymerase II promoter; sensory perception of pain; vein smooth muscle contraction; vasodilation; enteric nervous system development; response to organic cyclic substance; regulation of epithelial cell proliferation; elevation of cytosolic calcium ion concentration; regulation of blood pressure; positive regulation of cell proliferation; melanocyte differentiation; regulation of pH; neural crest cell migration; aging; vasoconstriction; posterior midgut development; macrophage chemotaxis; peripheral nervous system development; G-protein coupled receptor protein signaling pathway; negative regulation of neuron maturation; pigmentation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of protein amino acid phosphorylation; regulation of sensory perception of pain; negative regulation of apoptosis
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Research Articles on Ednrb

1. Renal expression of Ednrb correlated with captopril-induced blood pressure effects.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Ednrb antibody

 Products by Pathway  Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram

Diseases associated with anti-Ednrb antibody

 Disease Name  Pubmed Publications
 Hirschsprung Disease Antibodies  >140 publications with Ednrb and Hirschsprung Disease
 Nervous System Diseases Antibodies  >43 publications with Ednrb and Nervous System Diseases
 Disease Models, Animal Antibodies  >33 publications with Ednrb and Disease Models, Animal
 Inflammation Antibodies  >9 publications with Ednrb and Inflammation
 Lung Diseases Antibodies  >9 publications with Ednrb and Lung Diseases
 Urinary Bladder Neoplasms Antibodies  >8 publications with Ednrb and Urinary Bladder Neoplasms
 Neoplasms, Experimental Antibodies  >8 publications with Ednrb and Neoplasms, Experimental
 WAARDENBURG SYNDROME, TYPE 4A Antibodies  >7 publications with Ednrb and WAARDENBURG SYNDROME, TYPE 4A
 Cardiovascular Diseases Antibodies  >7 publications with Ednrb and Cardiovascular Diseases
 Hypertension Antibodies  >6 publications with Ednrb and Hypertension

Organs/Tissues associated with anti-Ednrb antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >18 publications with Ednrb and Brain
 Embryonic Tissue Antibodies  >16 publications with Ednrb and Embryonic Tissue
 Eye Antibodies  >15 publications with Ednrb and Eye
 Lung Antibodies  >11 publications with Ednrb and Lung
 Inner Ear Antibodies  >7 publications with Ednrb and Inner Ear
 Placenta Antibodies  >4 publications with Ednrb and Placenta
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