• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NESTIN (NES) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224038

MBS224038 recognises human Nestin, an intermediate filament involved in cytoskeletal organisation. Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.… More details

$860 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NESTIN, Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN NESTIN
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine, Dog, Hamster, Macaque, Mouse, Rabbit, Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5mg/ml (lot specific)
Specificity
This item recognises human Nestin, an intermediate filament involved in cytoskeletal organisation.
Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.
Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
This item has been reported to work in Immunofluorescence.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
201,770 Da
3D Structure
ModBase 3D Structure for Q6P5H2
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human Nestin (aa1484-1500).
Histology Positive Control Tissue||Human kidney
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to Human Nestin were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-Nes antibody
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-Nes antibody
ELISA: Minimum Dilution: 1/20000; Maximum Dilution: 1/80000;
Immunohistology - Paraffin: Maximum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/3000

Testing Data #1 of anti-Nes antibody

Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human nestin (MBS224038)
Rabbit Anti-NES Polyclonal Antibody – Cat# MBS224038

Testing Data #2 of anti-Nes antibody

Staining of formalin fixed, paraffin embedded mouse kidney with Rabbit anti Human nestin (MBS224038)
Rabbit Anti-NES Polyclonal Antibody, Testing Data #2 – Cat# MBS224038

Other Notes

Small volumes of anti-Nes antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nes purchase

MBS224038 is an antibody targeting Nes. Also known as RABBIT ANTI HUMAN NESTIN. Reactive in Bovine, Dog, Hamster, Macaque, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Nes. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
Nes
NCBI Official Synonym Symbols
C78523; ESTM46; AA166324
UniProt Protein Name
Nestin
Protein Family
UniProt Gene Name
Nes
UniProt Entry Name
NEST_MOUSE
NCBI Protein Information
nestin; intermediate filament protein

UniProt Comments for Nes

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Cellular Component: cytoplasm; intermediate filament; intracellular

Molecular Function: protein binding; structural molecule activity; CCR5 chemokine receptor binding

Biological Process: nervous system development; multicellular organismal development; cell projection morphogenesis; negative regulation of neuron apoptosis
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000