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ATG16 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224040

Rabbit anti ATG16 (Internal Region) antibody recognizes human Autophagy-related protein 16 (ATG16). ATG16 is a member of the autophagy protein family and forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ATG16, Polyclonal Antibody
Full Product Name
RABBIT ANTI ATG16 (INTERNAL REGION)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
49,486 Da
OMIM
610767
3D Structure
ModBase 3D Structure for Q676U5
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an 18 aa sequence from near the centre of human ATG16.
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antiserum to human ATG16 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-ATG16 antibody
Immunohistology Frozen, Western Blot (WB)
Application Notes for anti-ATG16 antibody
Western Blot: This item detects a band of approximately 75 kDa in HeLa cell lysate.
Western Blotting: Minimum Dilution: 0.25; Maximum Dilution: 0.5ug/ml

Testing Data #1 of anti-ATG16 antibody

Western blot analysis of whole cell lysate from HeLa adenocarcinoma cells probed with Rabbit anti Human ATG16 (MBS224040) at 0.25(A) and 0.5(B) ug/ml
Rabbit Anti-ATG16 Polyclonal Antibody – Cat# MBS224040

Testing Data #2 of anti-ATG16 antibody

Immunocytochemical staining of HeLa cells with Rabbit anri Human ATG16 (MBS224040)
Rabbit Anti-ATG16 Polyclonal Antibody, Testing Data #2 – Cat# MBS224040

Other Notes

Small volumes of anti-ATG16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG16 purchase

MBS224040 is an antibody targeting ATG16. Also known as RABBIT ANTI ATG16 (INTERNAL REGION). Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG16. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
autophagy-related protein 16-1 isoform 4
NCBI Official Synonym Full Names
autophagy related 16-like 1 (S. cerevisiae)
NCBI Official Symbol
ATG16L1
NCBI Official Synonym Symbols
IBD10; WDR30; APG16L; ATG16A; ATG16L
UniProt Protein Name
Autophagy-related protein 16-1
UniProt Synonym Protein Names
APG16-like 1
Protein Family
UniProt Gene Name
ATG16L1
UniProt Synonym Gene Names
APG16L
UniProt Entry Name
A16L1_HUMAN
NCBI Protein Information
autophagy-related protein 16-1; APG16L beta; ATG16 autophagy related 16-like 1; WD repeat domain 30

NCBI Summary for ATG16

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]
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UniProt Comments for ATG16

ATG16: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, peripheral; Autophagy

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: identical protein binding; protein binding

Biological Process: protein transport; protein homooligomerization; autophagic vacuole formation

Disease: Inflammatory Bowel Disease 10
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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