• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

AIF1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224105

Goat anti AIF1 antibody recognizes human allograft inflammatory factor 1 (AIF1), otherwise known as Ionized calcium-binding adapter molecule 1 (IBA1). AIF1 is a 147 amino acid ~17kDa actin-binding protein involved in macrophage activation, vascular smooth… More details

$775 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AIF1, Polyclonal Antibody
Full Product Name
GOAT ANTI AIF1 (C-TERMINAL)
Product Gene Name
anti-AIF1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Mouse, Pig (Expected from Sequence), Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
16,827 Da
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Buffer Solution
TRIS buffered saline
Antiserum Preparation
Antiserum to human AIF1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-AIF1 antibody
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-AIF1 antibody
Western Blot: This item detects a band of approximately 16kDa in rat brain cell lysates.
ELISA: Minimum Dilution: 1/32000
Immunohistology - Paraffin: Minimum Dilution: 2.0ug/ml; Application Note: This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Tris/EDTA buffer pH9.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 3.0ug/ml

Testing Data #1 of anti-AIF1 antibody

Rat brain lysate probed with Goat anti AIF1 (MBS224105)
Goat Anti-AIF1 Polyclonal Antibody – Cat# MBS224105

Other Notes

Small volumes of anti-AIF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AIF1 purchase

MBS224105 is an antibody targeting AIF1. Also known as GOAT ANTI AIF1 (C-TERMINAL). Reactive in Mouse, Pig (Expected from Sequence), Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for AIF1. It may not necessarily be applicable to this product.

NCBI GI #
8392881
NCBI GeneID
29427
NCBI Accession #
NP_058892.1
UniProt Secondary Accession #
P55007; P70491
UniProt Related Accession #
P55009
GenBank Nucleotide #
NM_017196.3
NCBI Official Full Name
allograft inflammatory factor 1
NCBI Official Synonym Full Names
allograft inflammatory factor 1
NCBI Official Symbol
Aif1
NCBI Official Synonym Symbols
iba1; Bart1; mrf-1; BART-1
UniProt Protein Name
Allograft inflammatory factor 1
UniProt Synonym Protein Names
Ionized calcium-binding adapter molecule 1; MRF-1; Microglia response factor
Protein Family
Allograft inflammatory factor
UniProt Gene Name
Aif1
UniProt Synonym Gene Names
Iba1; Mrf1; AIF-1
UniProt Entry Name
AIF1_RAT
NCBI Protein Information
allograft inflammatory factor 1; AIF-1; balloon angioplasty responsive transcript; balloon angioplasty-responsive transcript 1; ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule 1; microglia response factor; protein BART-1

NCBI Summary for AIF1

may play a role in macrophage activation and function [RGD, Feb 2006]

UniProt Comments for AIF1

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Cellular Component: ruffle; cell projection; perinuclear region of cytoplasm; lamellipodium; cytoplasm; actin filament; perikaryon; intracellular; cytosol; nucleus; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; Rac protein signal transduction; cellular response to hormone stimulus; macrophage activation; actin filament polymerization; response to cytokine stimulus; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; response to axon injury; positive regulation of protein amino acid phosphorylation; inflammatory response; response to electrical stimulus; phagocytosis, engulfment; positive regulation of cell migration; negative regulation of apoptosis
Show more

Research Articles on AIF1

1. The responses suggest that depending on the type of lesion DRG Iba-1 (+) resident macrophages have different activation mechanisms, which are dissimilar to those in microglia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-AIF1 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >280 publications with AIF1 and Disease Models, Animal
 Inflammation Antibodies  >157 publications with AIF1 and Inflammation
 Death Antibodies  >115 publications with AIF1 and Death
 Cardiovascular Diseases Antibodies  >99 publications with AIF1 and Cardiovascular Diseases
 Necrosis Antibodies  >93 publications with AIF1 and Necrosis
 Neurotoxicity Syndromes Antibodies  >17 publications with AIF1 and Neurotoxicity Syndromes
 Cognition Disorders Antibodies  >16 publications with AIF1 and Cognition Disorders
 Edema Antibodies  >13 publications with AIF1 and Edema
 Parkinson Disease Antibodies  >13 publications with AIF1 and Parkinson Disease
 Learning Disorders Antibodies  >11 publications with AIF1 and Learning Disorders

Organs/Tissues associated with anti-AIF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >423 publications with AIF1 and Brain
 Eye Antibodies  >32 publications with AIF1 and Eye
 Liver Antibodies  >15 publications with AIF1 and Liver
 Spleen Antibodies  >14 publications with AIF1 and Spleen
 Testis Antibodies  >5 publications with AIF1 and Testis
 Adipose Tissue Antibodies  >3 publications with AIF1 and Adipose Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000