Goat Anti-MCL-1 Polyclonal Antibody – Cat# MBS224201

Human lung cell lysate probed with Goat anti Human Mcl-1 C-Terminal (MBS224201)

MCL-1 Antibody – Goat Polyclonal

Name: MCL-1 Antibody; Polyclonal; Goat; Bovine/Mouse/Pig/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS224201
Price: 710 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224201

MBS224201 specifically recognises an epitope within the C-Terminal (CT) region of human Mcl-1, otherwise known as Myeloid cell leukaemia sequence 1, a mitochondrial-associated anti-apoptotic member of the Bcl-2 family, which acts as both an enhancer of cell… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Mcl-1, Polyclonal Antibody

Full Product Name

GOAT ANTI HUMAN Mcl-1 (C-TERMINAL)

Product Gene Name

anti-Mcl-1 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Bovine (Expected from Sequence), Dog (Expected from Sequence), Mouse (Expected from Sequence), Pig (Expected from Sequence), Rat (Expected from Sequence)

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 0.5mg/ml (lot specific)

Specificity

This item specifically recognises an epitope within the C-Terminal (CT) region of human Mcl-1, otherwise known as Myeloid cell leukaemia sequence 1, a mitochondrial-associated anti-apoptotic member of the Bcl-2 family, which acts as both an enhancer of cell survival (isoform 1), and promoter of cell death (isoform 2).

Mcl-1 is expressed in many different cell lines, and several types of cancer, and blockage of its function, may prove to be a useful therapeutic agent in the treatment of malignancies and inflammatory and infectious diseases.

This item is expected to recognise the longer gene product, isoform 1.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

28,662 Da

OMIM

159552

3D Structure

ModBase 3D Structure for Q07820

Perservative Stabilisers

0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin

Immunogen

Synthetic peptide sequence C-AGVGAGLAYLIR from the C-Terminal region of Mcl-1 (NP_068779.1).
Buffer Solution||TRIS buffered saline

Antiserum Preparation

Antiserum to human Mcl-1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-Mcl-1 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-Mcl-1 antibody

Western Blot: This item detects a band of approximately 35kDa in human lung and K562 cell lysates.
ELISA: Minimum Dilution: 1/2000
Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 3.0ug/ml

Other Notes

Small volumes of anti-Mcl-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mcl-1 purchase

MBS224201 is an antibody targeting Mcl-1. Also known as GOAT ANTI HUMAN Mcl-1 (C-TERMINAL). Reactive in Bovine (Expected from Sequence), Dog (Expected from Sequence), Mouse (Expected from Sequence), Pig (Expected from Sequence), Rat (Expected from Sequence). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for Mcl-1. It may not necessarily be applicable to this product.

NCBI GI #

309747067

NCBI GeneID

4170

NCBI Accession #

NP_001184249.1

UniProt Primary Accession #

Q07820

UniProt Secondary Accession #

Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04

UniProt Related Accession #

Q07820

GenBank Nucleotide #

NM_001197320.1

NCBI Official Full Name

induced myeloid leukemia cell differentiation protein Mcl-1 isoform 3

NCBI Official Synonym Full Names

myeloid cell leukemia 1

NCBI Official Symbol

MCL1

NCBI Official Synonym Symbols

TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

UniProt Protein Name

Induced myeloid leukemia cell differentiation protein Mcl-1

UniProt Synonym Protein Names

Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT

Protein Family

Induced myeloid leukemia cell differentiation protein

UniProt Gene Name

MCL1

UniProt Synonym Gene Names

BCL2L3; Bcl2-L-3

UniProt Entry Name

MCL1_HUMAN

NCBI Protein Information

induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related)

NCBI Summary for Mcl-1

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

UniProt Comments for Mcl-1

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Inhibitor; Apoptosis; Mitochondrial; Channel, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cellular homeostasis; cell fate determination

Research Articles on Mcl-1

1. Results position TWEAK-Fn14 signaling through Mcl-1 as a significant mechanism for NSCLC tumor cell survival.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Mcl-1 antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
E2F Transcription Factor Network Pathway antibodies	E2F Transcription Factor Network Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
IL-7 Signaling Pathway antibodies	IL-7 Signaling Pathway Diagram
IL6-mediated Signaling Events Pathway antibodies	IL6-mediated Signaling Events Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram

Diseases associated with anti-Mcl-1 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>60 publications with Mcl-1 and Adenocarcinoma
Lung Neoplasms Antibodies	>56 publications with Mcl-1 and Lung Neoplasms
Necrosis Antibodies	>49 publications with Mcl-1 and Necrosis
Breast Neoplasms Antibodies	>42 publications with Mcl-1 and Breast Neoplasms
Liver Diseases Antibodies	>38 publications with Mcl-1 and Liver Diseases
Liver Neoplasms Antibodies	>32 publications with Mcl-1 and Liver Neoplasms
Neoplasms, Experimental Antibodies	>32 publications with Mcl-1 and Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>30 publications with Mcl-1 and Carcinoma, Hepatocellular
Inflammation Antibodies	>28 publications with Mcl-1 and Inflammation
Multiple Myeloma Antibodies	>28 publications with Mcl-1 and Multiple Myeloma

Organs/Tissues associated with anti-Mcl-1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>150 publications with Mcl-1 and Blood
Lung Antibodies	>79 publications with Mcl-1 and Lung
Liver Antibodies	>70 publications with Mcl-1 and Liver
Bone Antibodies	>60 publications with Mcl-1 and Bone
Bone Marrow Antibodies	>44 publications with Mcl-1 and Bone Marrow
Brain Antibodies	>39 publications with Mcl-1 and Brain
Prostate Antibodies	>30 publications with Mcl-1 and Prostate
Vascular Antibodies	>22 publications with Mcl-1 and Vascular
Skin Antibodies	>19 publications with Mcl-1 and Skin
Lymph Node Antibodies	>15 publications with Mcl-1 and Lymph Node