Goat Anti-NOXA Polyclonal Antibody – Cat# MBS224213

Human cerebellum cell lysate probed with Goat anti Human Noxa (MBS224213)

NOXA Antibody – Goat Polyclonal

Name: NOXA Antibody; Polyclonal; Goat; MyBioSource
Brand: MyBioSource
SKU: MBS224213
Price: 745 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224213

MBS224213 specifically recognises human Noxa, a mitochondrial localised protein and member of the BH3-only proapoptotic protein family, which is up-regulated by p53, IFNbeta, phorbol esters, dsRNA and viruses, and acts as promoter of caspase and apoptosis… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

NOXA, Polyclonal Antibody

Full Product Name

GOAT ANTI HUMAN NOXA

Product Gene Name

anti-NOXA antibody

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 0.5mg/ml (lot specific)

Specificity

This item specifically recognises human Noxa, a mitochondrial localised protein and member of the BH3-only proapoptotic protein family, which is up-regulated by p53, IFNbeta, phorbol esters, dsRNA and viruses, and acts as promoter of caspase and apoptosis activation.

Noxa is a transcriptional target of p53, p73 and BH3-only proteins, and competes with, and displaces, the proapoptotic proteins BAK1, and BIM, from the antiapoptotioc protein MCL1 (Bcl-2-related protein EAT/mcl1), thereby influencing apoptosis versus cell survival.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

14,955 Da

OMIM

604959

3D Structure

ModBase 3D Structure for Q13794

Perservative Stabilisers

0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin

Immunogen

Synthetic peptide sequence C-TQLRRFGDKLNFRQK from the internal region of Noxa (NP_066950.1).
Buffer Solution||TRIS buffered saline

Antiserum Preparation

Antiserum to human NOXA was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-NOXA antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-NOXA antibody

Western Blot: This item detects a band of approximately 16kDa in human cerebellum cell lysates. Suitable loading control: Goat anti Human GAPDH
ELISA: Minimum Dilution: 1/16000
Western Blotting: Minimum Dilution: 0.03; Maximum Dilution: 0.1ug/ml

Other Notes

Small volumes of anti-NOXA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOXA purchase

MBS224213 is an antibody targeting NOXA. Also known as GOAT ANTI HUMAN NOXA. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for NOXA. It may not necessarily be applicable to this product.

NCBI GI #

10863923

NCBI GeneID

5366

NCBI Accession #

NP_066950.1

UniProt Primary Accession #

Q13794

UniProt Secondary Accession #

Q8N589; B2R4T7

UniProt Related Accession #

Q13794

GenBank Nucleotide #

NM_021127.2

NCBI Official Full Name

phorbol-12-myristate-13-acetate-induced protein 1

NCBI Official Synonym Full Names

phorbol-12-myristate-13-acetate-induced protein 1

NCBI Official Symbol

PMAIP1

NCBI Official Synonym Symbols

APR; NOXA

UniProt Protein Name

Phorbol-12-myristate-13-acetate-induced protein 1

UniProt Synonym Protein Names

Immediate-early-response protein APR; Protein Noxa

Protein Family

Superoxide-generating NADPH oxidase

UniProt Gene Name

PMAIP1

UniProt Synonym Gene Names

NOXA; PMA-induced protein 1

UniProt Entry Name

APR_HUMAN

NCBI Protein Information

phorbol-12-myristate-13-acetate-induced protein 1; PMA-induced protein 1; adult T cell leukemia-derived PMA-responsive; immediate-early-response protein APR; protein Noxa

UniProt Comments for NOXA

NOXA: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. Interacts with MCL1, BCL2A1 and BAX. Up-regulated by p53/TP53, phorbol esters, double- stranded RNA, IFNB1/IFN-beta and viruses. Highly expressed in adult T-cell leukemia cell line. Belongs to the PMAIP1 family.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 18q21.32

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol; nucleus

Molecular Function: protein binding

Biological Process: proteasomal protein catabolic process; caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; response to dsRNA; positive regulation of caspase activity; cellular response to glucose starvation; positive regulation of protein oligomerization; negative regulation of fibroblast proliferation; positive regulation of neuron apoptosis; T cell homeostasis; regulation of mitochondrial membrane permeability; positive regulation of DNA damage response, signal transduction by p53 class mediator; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to X-ray; response to UV

Research Articles on NOXA

1. Overexpression of ApoL2 did not induce cell death on its own. ApoL2 did not sensitize or protect cells from overexpression of the BH3-only proteins Bmf or Noxa.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NOXA antibody

Reference Product	PubMed Publications
BBC3 antibody	>73 publications with NOXA and BBC3
BCL2L1 antibody	>49 publications with NOXA and BCL2L1
MCL1 antibody	>46 publications with NOXA and MCL1
TP53 antibody	>33 publications with NOXA and TP53
BID antibody	>27 publications with NOXA and BID
BAD antibody	>24 publications with NOXA and BAD
MYC antibody	>17 publications with NOXA and MYC
POU4F1 antibody	>1 publications with NOXA and POU4F1

Pathways associated with anti-NOXA antibody

Products by Pathway	Pathway Diagram
Activation Of BH3-only Proteins Pathway antibodies	Activation Of BH3-only Proteins Pathway Diagram
Activation Of NOXA And Translocation To Mitochondria Pathway antibodies	Activation Of NOXA And Translocation To Mitochondria Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway antibodies	BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway Diagram
DNA Damage Response Pathway antibodies	DNA Damage Response Pathway Diagram
DNA Damage Response (only ATM Dependent) Pathway antibodies	DNA Damage Response (only ATM Dependent) Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Intrinsic Pathway For Apoptosis antibodies	Intrinsic Pathway For Apoptosis Diagram

Diseases associated with anti-NOXA antibody

Disease Name	Pubmed Publications
Melanoma Antibodies	>31 publications with NOXA and Melanoma
Leukemia, Lymphocytic, Chronic, B-Cell Antibodies	>28 publications with NOXA and Leukemia, Lymphocytic, Chronic, B-Cell
Necrosis Antibodies	>23 publications with NOXA and Necrosis
Breast Neoplasms Antibodies	>21 publications with NOXA and Breast Neoplasms
Cardiovascular Diseases Antibodies	>21 publications with NOXA and Cardiovascular Diseases
Lung Neoplasms Antibodies	>20 publications with NOXA and Lung Neoplasms
Multiple Myeloma Antibodies	>19 publications with NOXA and Multiple Myeloma
Nervous System Diseases Antibodies	>19 publications with NOXA and Nervous System Diseases
Lung Diseases Antibodies	>15 publications with NOXA and Lung Diseases
Inflammation Antibodies	>13 publications with NOXA and Inflammation

Organs/Tissues associated with anti-NOXA antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>63 publications with NOXA and Blood
Lung Antibodies	>28 publications with NOXA and Lung
Brain Antibodies	>18 publications with NOXA and Brain
Bone Antibodies	>16 publications with NOXA and Bone
Skin Antibodies	>14 publications with NOXA and Skin
Liver Antibodies	>13 publications with NOXA and Liver
Bone Marrow Antibodies	>11 publications with NOXA and Bone Marrow
Lymph Node Antibodies	>11 publications with NOXA and Lymph Node
Embryonic Tissue Antibodies	>9 publications with NOXA and Embryonic Tissue
Prostate Antibodies	>8 publications with NOXA and Prostate