• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD294 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224236

MBS224236 recognises CD294, also known as chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) and GRP44. CD294 is a G protein-coupled receptor which has a high affinity for prostaglandin D2 (PGD2). CD294 is predominantly expressed on… More details

$750 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD294, Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN CD294 (N-TERMINAL)
Product Gene Name
anti-CD294 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse (Expected from Sequence), Rat (Expected from Sequence)
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
This item recognises CD294, also known as chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) and GRP44.

CD294 is a G protein-coupled receptor which has a high affinity for prostaglandin D2 (PGD2).

CD294 is predominantly expressed on Th2 cells, basophils and eosinophils, and its activation by PDG2 mediates chemotaxis of these cells to inflammatory sites. CD294 may be a useful marker for Th2 cells, and enhances their function and signalling with a possible role in Th2 cell differentiation.

PGD2 is thought to contribute to bronchoconstriction, eosinophilia and mucus production in allergic asthma, and CD294 may contribute to this disease.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
Molecular Weight
42,950 Da
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to an 18 amino acid sequence from near the amino terminus of human CD294 - KLH conjugated.
Histology Positive Control Tissue||Human adrenal gland
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to human CD294 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-CD294 antibody
ELISA (EIA), Immunohistology Paraffin, Western Blot (WB)
Application Notes for anti-CD294 antibody
Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Immunohistology - Paraffin: Minimum Dilution: 5ug/ml

Testing Data #1 of anti-CD294 antibody

Staining of formalin fixed, paraffin embedded human brain cortex with Rabbit anti Human CD294 (MBS224236)
Rabbit Anti-CD294 Polyclonal Antibody – Cat# MBS224236

Testing Data #2 of anti-CD294 antibody

Staining of formalin fixed, paraffin embedded human adrenal gland with Rabbit anti Human CD294 (MBS224236)
Rabbit Anti-CD294 Polyclonal Antibody, Testing Data #2 – Cat# MBS224236

Testing Data #3 of anti-CD294 antibody

Staining of formalin fixed, paraffin embedded human cerebllum with Rabbit anti Human CD294 (MBS224236)
Rabbit Anti-CD294 Polyclonal Antibody, Testing Data #3 – Cat# MBS224236

Testing Data #4 of anti-CD294 antibody

Western blotting of Jurkat cell lysate probed with Rabbit anti Human CD294 (MBS224236)
Rabbit Anti-CD294 Polyclonal Antibody, Testing Data #4 – Cat# MBS224236

Other Notes

Small volumes of anti-CD294 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD294 purchase

MBS224236 is an antibody targeting CD294. Also known as RABBIT ANTI HUMAN CD294 (N-TERMINAL). Reactive in Mouse (Expected from Sequence), Rat (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD294. It may not necessarily be applicable to this product.

NCBI GI #
6753528
NCBI GeneID
14764
NCBI Accession #
NP_034092.1
UniProt Secondary Accession #
Q54A00
UniProt Related Accession #
Q9Z2J6
GenBank Nucleotide #
NM_009962.3
NCBI Official Full Name
prostaglandin D2 receptor 2
NCBI Official Synonym Full Names
prostaglandin D2 receptor 2
NCBI Official Symbol
Ptgdr2
NCBI Official Synonym Symbols
Crth2; Gpr44; Grp45
UniProt Protein Name
Prostaglandin D2 receptor 2
UniProt Synonym Protein Names
Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CD_antigen: CD294, Gpr44
Protein Family
Prostaglandin D2 receptor
UniProt Gene Name
Ptgdr2
UniProt Synonym Gene Names
Crth2
UniProt Entry Name
PD2R2_MOUSE
NCBI Protein Information
prostaglandin D2 receptor 2; G protein-coupled receptor 44; G-protein coupled receptor 44; PGD2 receptor; chemoattractant receptor-homologous molecule expressed on TH2 cells; prostaglandin D2 receptor CRTH2

UniProt Comments for CD294

GPR44: Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin- sensitive decreases in cAMP levels and Ca(2+) mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, ADRBK1/GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Cellular Component: neuron projection; membrane; integral to plasma membrane; plasma membrane; integral to membrane; intracellular

Molecular Function: G-protein coupled receptor activity; neuropeptide binding; signal transducer activity; prostaglandin F receptor activity; prostaglandin J receptor activity; prostaglandin D receptor activity

Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; behavior; positive regulation of G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; G-protein signaling, adenylate cyclase inhibiting pathway; signal transduction; chemotaxis
Show more

Research Articles on CD294

1. Prostaglandin D2 acts through the Dp2 receptor to influence male germ cell differentiation in the foetal mouse testis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD294 antibody

 Reference Product  PubMed Publications
 Ccr3 antibody  >1 publications with CD294 and Ccr3

Pathways associated with anti-CD294 antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eicosanoid Ligand-binding Receptors Pathway antibodies  Eicosanoid Ligand-binding Receptors Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Metabolic Disorders Of Biological Oxidation Enzymes Pathway antibodies  Metabolic Disorders Of Biological Oxidation Enzymes Pathway Diagram
 Odorant GPCRs Pathway antibodies  Odorant GPCRs Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000